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European Journal of Nuclear Medicine

, Volume 21, Issue 4, pp 314–321 | Cite as

Pre-targeted immunodetection in glioma patients: tumour localization and single-photon emission tomography imaging of [99mTc]PnAO-biotin

  • Giovanni Paganelli
  • Patrizia Magnani
  • Felicia Zito
  • Giovanni Lucignan
  • Francesco Sudati
  • Giulio Truci
  • Enrico Motti
  • Mariarosa Terreni
  • Bianca Pollo
  • Massimo Giovanelli
  • Nicola Canal
  • Giuseppe Scotti
  • Giancarlo Comi
  • Peter Koch
  • Haelmut R. Maecke
  • Ferruccio Fazio
Original Articles

Abstract

The imaging of cerebral gliomas with radiolabelled monoclonal antibodies (MoAbs) has been previously reported. However, previous studies have been hampered by the drawback of a low tumour to non-tumour ratio. In order to overcome this problem we have developed a three-step pre-targeting method using the avidin-biotin system. The rationale of this technique consists in vivo labelling of biotinylated MoAbs targeted onto tumour deposits, when most of the unbound antibodies have been cleared from the bloodstream as avidin-bound complexes. The anti-tenascin MoAb BC2, specific for the majority of gliomas, was biotinylated and 1 mg was administered i.v. in 20 patients with histologically documented cerebral lesions. After 24–36 h, 5 mg avidin was injected i.v. followed 24 h later by a third i.v. injection of 0.2 mg PnAO-biotin labelled with 15–20 tnCi technetium-99m. No evidence of toxicity was observed. Whole-body biodistribution was measured at 20 min, 3 h and 5 h post-injection. [99mTc]PnAO-bio-tin had a fast blood clearance and was primarily excreted through the biliary system. A dedicated single-photon emission tomography system was used to acquire brain tomographic images 1–2 h after the administration of [99mTc]PnAO-biotin. Tumours were detected in 15/18 glioma patients with a tumour to non-tumour ratio of up 14:1. This three-step method, based on the sequential administration of anti-tenascin MoAb BC2, avidin and [99mTc]PnAO-biotin, can support computed tomography or magnetic resonance imaging for the diagnosis and follow-up of patients with glioma. Further studies are required to evaluate the potential of this technique for therapeutic application.

Key words

Monoclonal antibodies Avidin Biotin Tenascin Emission tomography 

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Giovanni Paganelli
    • 1
  • Patrizia Magnani
    • 1
  • Felicia Zito
    • 1
  • Giovanni Lucignan
    • 1
  • Francesco Sudati
    • 1
  • Giulio Truci
    • 2
  • Enrico Motti
    • 3
  • Mariarosa Terreni
    • 4
  • Bianca Pollo
    • 5
  • Massimo Giovanelli
    • 3
  • Nicola Canal
    • 2
  • Giuseppe Scotti
    • 6
  • Giancarlo Comi
    • 2
  • Peter Koch
    • 7
  • Haelmut R. Maecke
    • 7
  • Ferruccio Fazio
    • 1
  1. 1.Department of Nuclear Medicine, Scientific Institute H San RaffaeleINB-CNR, University of MilanMilanoItaly
  2. 2.Division of NeurologyUniversity of Milan, Scientific Institute H San RaffaeleMilanoItaly
  3. 3.Division of NeurosurgeryUniversity of Milan, Scientific Institute H San RaffaeleMilanoItaly
  4. 4.Department of PathologyScientific Institute H San RaffaeleMilanoItaly
  5. 5.Department of PathologyScientific Institute G. BestaMilanoItaly
  6. 6.Department of NeuroradiologyUniversity of Milan, Scientific Institute H San RaffaeleMilanoItaly
  7. 7.Department of Nuclear MedicineKantonsspital, University of BaselBaselSwitzerland

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