Urological Research

, Volume 25, Issue 3, pp 207–211 | Cite as

Effect of ischemia and partial outflow obstruction on rat bladder function

  • M. Saito
  • K. Yokoi
  • M. Ohmura
  • A. Kondo
Original Paper


We investigated the effects of ischemia induced by ligation of the bilateral internal iliac arteries following partial outlet obstruction on changes in detrusor function in rat. Rats were divided into three groups: sham-operated control rats, rats with partial outlet obstruction, and rats with obstruction + ischemia. Bladder function was studied by the in vitro organ bath technique 7 days after surgery. The weight of the bladder was significantly increased in both the obstruction and obstruction + ischemia groups. The obstruction + ischemia group exhibited a greater increase in weight. The passive length-tension relationship of detrusor muscle strips showed that tissue elasticity was decreased and the active length-tension relationship demonstrated that the peak response was observed at a shorter tissue length in the obstruction + ischemia group compared with the other two groups. There was no difference in the passive and active length-tension relationships between the control group and the obstruction group. The contractile response to various kinds of stimulation (field stimulation, bethanechol, ATP, and KCl) increased in the obstruction group and decreased in the obstruction + ischemia group. These findings suggest that partial outflow obstruction alone increased bladder contractility in response to stimuli. However, ischemia reduced the contractility and elasticity of the bladder wall.

Key words

Outflow obstruction Rat bladder Ischemia Detrusor function Atherosclerosis Benign prostatic hyperplasia 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Andersson PO, Malmgren A, Uvelius B (1988) Cystometrical and in vitro evaluation of urinary bladder function of rats with streptozotocin-induced diabetes. J Urol 139:1359PubMedGoogle Scholar
  2. 2.
    Dunn M (1974) A study of the bladder blood flow during distension in rabbit. Br J Urol 47:67Google Scholar
  3. 3.
    Finkbeiner A, Lapides J (1974) Effect of distension on blood flow in dog's urinary bladder. Invest Urol 12:210PubMedGoogle Scholar
  4. 4.
    Gosling JA, Gilpin SA, Dixon JS, Gilpin CJ (1986) Decrease in the autonomic innervation of human detrusor muscle in outflow obstruction. J Urol 136:501PubMedGoogle Scholar
  5. 5.
    Grayhack IT, Kozlowski JM (1987) Benign prostatic hyperplasia. In: Gillenwater JY, Grayhack JT, Howards SS, Duckett JW (eds) Adult and pediatric urology. Year Book Medical, Chicago, p 1062Google Scholar
  6. 6.
    Kato K, Wein AJ, Kitada S, Haugaard N, Levin RM (1988) The functional effect of mild outlet obstruction on the rabbit urinary bladder. J Urol 140:880PubMedGoogle Scholar
  7. 7.
    Levin RM, Longhurst PA, Monson FC, Kato K, Wein AJ (1990) Effect of bladder outlet obstruction on the morphology, physiology, and pharmacology of the bladder. Prostate 3 (suppl):9Google Scholar
  8. 8.
    Levin RM, Saito M, Wein AJ, Packard D, Choen A, Haugaard N (1993) Effect of partial outlet obstruction on choline acetyltransferase activity in the rat and rabbit. Neurourol Urodyn 12:255PubMedGoogle Scholar
  9. 9.
    Lin AT, Yang CH, Chen MT, Chang LS (1993) Recovery of blood flow and bioenergetics of the obstructed rabbit urinary bladder after relieving outlet obstruction. Neurourol Urodyn 12:379Google Scholar
  10. 10.
    Mattiasson A, Ekstrom J, Larsson B (1987) Changes in the nervous control of the rat urinary bladder induced by outflow obstruction. Neurourol Urodyn 6:37Google Scholar
  11. 11.
    Methora RML (1953) An experimental study of the vesical circulation during distension and in cystitis. J Pathol Bact 66:79Google Scholar
  12. 12.
    Nielsen KK, Nielsen SL, Nordling J, Kromann-Andersen B (1991) Rate of urinary bladder blood flow evaluated by133Xe washout and radioactive microspheres in pigs. Urol Res 19:378Google Scholar
  13. 13.
    Ohmura M, Yokoi K, Kondo A, Miyake K, Saito M (1996) Effects of ischemia on the function of the isolated rat detrusor muscle. Acta Urol Jpn 42:111Google Scholar
  14. 14.
    Saito M, Kondo A, Kato T, Hasegawa S, Miyake K (1993) Response of the human neurogenic bladder to KCI, carbachol, ATP Ad CaCl2. Rr T Urnl 72: 298Google Scholar
  15. 15.
    Saito M, Longhurst PA, Tammela TLJ, Wein AJ, Levin RM (1993) Effect of partial outlet obstruction of the rat urinary bladder on micturition characteristics, DNA synthesis, and the contractile response to field stimulation and pharmacological agents. J Urol 150:1045PubMedGoogle Scholar
  16. 16.
    Saito M, Wein AJ, Levin RM (1993) Effect of partial outlet obstruction on contractility: comparison between severe and mild obstruction. Neurourol Urodyn 12:57Google Scholar
  17. 17.
    Saito M, Ohmura M, Kondo A (1996) Effects of long-term partial outflow obstruction on bladder function in the rat. Neurourol Urodyn 15:157Google Scholar
  18. 18.
    Siroky MB, Krane RJ (1990) An experimental model to study vesical blood flow. Neurourol Urodyn 9:197Google Scholar
  19. 19.
    Sjogren C, Andersson K-E, Husted S, Mattiasson A, MollerMadsen A (1978) Atropine resistance of transmurally stimulated isolated human bladder muscle. J Urol 128:1368Google Scholar
  20. 20.
    Turner-Warwick R, Whiteside CG, Arnold EP, Bates CP, WorthPHL Milroy EGJ, Webster JR, Weir J (1973) A urodynamic view of prostatic obstruction and the results of prostatectomy. Br J Urol 45:631PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • M. Saito
    • 1
  • K. Yokoi
    • 1
  • M. Ohmura
    • 1
  • A. Kondo
    • 1
  1. 1.Department of UrologyNagoya University School of MedicineShowa-ku, NagoyaJapan

Personalised recommendations