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Plant Systematics and Evolution

, Volume 183, Issue 3–4, pp 223–234 | Cite as

Structure and organization of the 5S rRNA genes (5S DNA) inPinus radiata (Pinaceae)

  • Susan Wilkie Gorman
  • Robert Dixon Teasdale
  • Christopher Ashley Cullis
Article

Abstract

A 5S rRNA gene (5S DNA) from the coniferPinus radiataD. Don has been cloned and characterized at the nucleotide, genomic and chromosomal levels. Sequencing revealed a repeat unit of 524 base pairs which is present in approximately 3000 copies per diploid genome. Two-dimensional gel electrophoresis indicated that these copies are organized in tandem arrays of various length. Using in situ hybridization techniques, the tandem arrays appear to be present on all of the chromosomes. This complexity of chromosomal organization contrasts markedly with the few sites of uniform length found in angiosperm plants such as wheat, pea, and maize.

Key words

Gymnosperms Pinaceae Pinus Dispersed repeats 5S rRNA two-dimensional gel electrophoresis in situ hybridizations 

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Susan Wilkie Gorman
    • 2
  • Robert Dixon Teasdale
    • 3
  • Christopher Ashley Cullis
    • 1
  1. 1.Case Western Reserve UniversityClevelandUSA
  2. 2.Southwestern medical Center at DallasUniversity of TexasDallasUSA
  3. 3.Division of Science and TechnologyGriffith UniversityQueenslandAustralia

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