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Comparative ultrastructure of pyrenoids inTrebouxia (Microthamniales, Chlorophyta)

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Abstract

Pyrenoid ultrastructure has been investigated from cultures of all 26 species ofTrebouxia with the aim of establishing pyrenoids as a taxonomic character. Different arrangements and forms of thylakoid lamellae within the pyrenoid matrix allow eight pyrenoid types to be distinguished. Each type is characteristic of a group of species. Thegigantea- andimpressa-type are similar, differing only in the form of the tubules: short, branched tubules mark thegigantea-type; ± long and straight invaginations theimpressa-type. Thearboricola-type is characterized by meandering pyrenoid membranes developing from lamellae parallel with each other in young autospores. Pyrenoids of thegelatinosa-type are traversed by thin parallel-arranged tubules. Few thylakoids with a curved profile are typical of theirregularis-type. Thecorticola-type is different from all others in having a distinct starch sheath closely connected with the pyrenoid matrix and no pyrenoglobuli being associated with the pyrenoid membranes. No true pyrenoids have been found inT. magna andT. erici. Within the chloroplast, they have indistinct areas with pyrenoglobuli, but without differentiated thylakoids. Pyrenoid morphology is stable in culture on different media as well as in phycobionts within lichen thalli. Comparing the pyrenoid of a lichenizedTrebouxia with that from cultured species, the identification of the phycobiont within the lichen thallus is possible, without the need of culturing the algae. This has been shown in species ofParmelia andHypogymnia. New aspects for the taxonomy and systematics ofTrebouxia are discussed.

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Dedicated to Prof. DrLothar Geitler on the occasion of the 90th anniversary of his birthday.

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Friedl, T. Comparative ultrastructure of pyrenoids inTrebouxia (Microthamniales, Chlorophyta). Plant Syst Evol 164, 145–159 (1989). https://doi.org/10.1007/BF00940435

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Key words

  • Algae
  • Chlorophyta
  • Trebouxia
  • Pseudotrebouxia
  • lichens
  • Parmelia
  • Hypogymnia
  • Pyrenoid
  • ultrastructure
  • systematics
  • taxonomy
  • phycobionts
  • freeze fixation
  • freeze substitution