Plant Systematics and Evolution

, Volume 189, Issue 3–4, pp 247–257 | Cite as

Evidence from RAPD markers in the evolution ofEchinochloa millets (Poaceae)

  • Khidir W. Hilu


Echinochloa (Poaceae) includes two domesticated species,Echinochloa utilis (Japanese barnyard millet) andE. frumentacea (Indian sawa millet) and 20–30 wild species. The two millets are morphologically very variable and overlap in spikelet and inflorescence characteristics. Both species are hexaploids based on x = 9. Cytogenetic studies point to the hexaploid wild speciesE. crusgalli andE. colona as possible progenitors ofE. utilis andE. frumentacea, respectively. The tetraploidE. oryzoides is considered as a possible genome donor to wild and domesticated barnyard millet. Markers from Random Amplified Polymorphic DNA method were used to assess the proposed phylogeny and examine the genetic diversity in both domesticated and wild species. The data were analyzed numerically.Echinochloa utilis andE. frumentacea appear very distinct, but grouped withE. crusgalli andE. colona, respectively. The tetraploidE. oryzoides show strong genetic affinity to theE. utilis—E. crusgalli group. The data are in general agreement with the cytogenetic information; however, some disagreements on the interpretation of some of the cytogenetic information is raised. The variability in DNA markers observed in the domesticated species, particularlyE. frumentacea, points to the feasibility of using RAPD markers in cultivar fingerprinting and breeding programs of these millets.

Key words

Poaceae Echinochloa sawa and barnyard millets RAPD analysis evolution genetic resources 


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  1. Clayton, W. D., Renvoize, S. A., 1986: Genera graminum. — Kew Bull. Add. Ser.13: 280–281.Google Scholar
  2. Demeke, T., Adams, R. P., Chibbar, R., 1992: Potential taxonomic use of random amplified polymorphic DNA (RAPD): a case study inBrassica. — Theor. Appl. Genet.84: 990–994.Google Scholar
  3. Devos, K. M., Gale, M. D., 1992: The use of random amplified polymorphic DNA markers in wheat. — Theor. Appl. Genet.84: 567–572.Google Scholar
  4. De Wet, J. M. J., Prasada Roa, K. E., Mengesha, M. H., Brink, D. E., 1983: Domestication of saw millet (Echinochloa colona). — Econ. Bot.37: 283–291.Google Scholar
  5. Dice, L. R., 1945: Measures of the amount of ecological association between species. — Ecology26: 295–302.Google Scholar
  6. Doggett, H., 1989: Small millets—a selective overview. — InSeetharam, A., Riley, K. W., Harinarayana, G., (Eds): Small millets in global agriculture, pp. 3–18. — New Delhi: Oxford & IBH Pub.Google Scholar
  7. Hilu, K. W., Johnson, J. L., 1991: Chloroplast DNA reassociation and grass phylogeny. — Pl. Syst. Evol.176: 21–31.Google Scholar
  8. Hu, J., Quiros, C. F., 1991: Identification of broccoli and cauliflower cultivars with RAPD markers. — Pl. Cell Reports10: 505–511.Google Scholar
  9. Klein-Lankhorst, V. A., Weide, R., Liharska, T., Zabel, P., 1991: Isolation of molecular markers for tomato (L. esculentum) using random amplified DNA (RAPD). — Theor. Appl. Genet.83: 108–114.Google Scholar
  10. Nei, M., Li, W. H., 1979: Mathematical model for studying genetic variation in terms of restrictions endonucleases. — Proc. Natl. Acad. Sci. USA76: 5269–5273.PubMedGoogle Scholar
  11. Rohlf, F. J., 1988: NTSYS-pc. Numerical taxonomy and multivariate analysis system. Version 1.5. — Setauket, NY: Exeter Publ.Google Scholar
  12. Saghai-Maroof, M. A., Soliman, K. M., Jorgensen, R. A., Allard, R. W., 1984: Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. — Proc. Natl. Acad. Sci. USA81: 8014–8018.PubMedGoogle Scholar
  13. Sokal, R. R., Michener, C. D., 1958: A statistical method for evaluating systematic relationships. — Univ. Kansas Sci. Bull.44: 467–507.Google Scholar
  14. Vierling, R. A., Nguyen, H. T., 1992: Use of RAPD markers to determine the genetic diversity of diploid, wheat genotypes. — Theor. Appl. Genet.84: 835–838.Google Scholar
  15. Williams, J. G. K., Kubelik, A. R., Rafaliski, K. J., Tingey, S. V., 1990: DNY polymerase amplified by arbitrary primers are useful as genetic markers. — Nucl. Acids Res.18: 6531–6535.PubMedGoogle Scholar
  16. Yabuno, T., 1962: Cytotaxonomic studies on the two cultivated species and the wild relatives in the genusEchinochloa. — Cytologia27: 296–305.Google Scholar
  17. , 1966: Biosystematic study of the genusEchinochloa. — Japan. J. Bot.19: 277–323.Google Scholar
  18. , 1973: Cytological relationships between someEchinochloa species and KenyanE. haploclada (Stapf)Stapf. — Cytologia38: 131–135.Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Khidir W. Hilu
    • 1
  1. 1.Department of BiologyVirginia Polytechnic Institute and State UniversityBlacksburgUSA

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