Journal of Neurology

, Volume 242, Supplement 1, pp S36–S39 | Cite as

Transplantation of purified populations of Schwann cells into lesioned adult rat spinal cord

  • Mary Bartlett Bunge


Both peripheral nerve and purified populations of Schwann cells promote axonal regeneration in the peripheral and central nervous systems. In order to assess whether Schwann cells can provide a bridge enabling regrowth of descending and ascending axons across an area of injury in adult spinal cord, Schwann cells enclosed within a collagen scroll were transplanted into lesions created photochemically. Numerous myelinated and unmyelinated axons were found throughout 28–90 day implants; Schwann cells myelinated or ensheathed the ingrowing axons normally. In contrast, acellular collagen grafts did not contain axons. Thus, Schwann cells stimulated abundant growth of axons into the grafts. In part to address the concern that the dense collagen layer acted as a barrier, we assessed transplantation of Schwann cells, inside semi-permeable polyacrylonitrile/polyvinylchloride (PAN/PVC) guidance channels, after transection of adult inbred rat spinal cords at T8 with removal of the the T9–11 segments. One month after grafting, a vascularized tissue cable was present with more myelinated and unmyelinated axons in the Schwann cell seeded channels than controls. Supraspinal axons did not invade the channel; some were of peripheral origin and others were spinal cord interneurons found up to nine segments away from the graft. When both cut ends of the cord were from inserted into Schwann cell filled channels, a vascularized tissue cable bridged the ends of the spinal cord, containing numerous myelinated axons and more unmyelinated axons, originating from spinal grey matter and dorsal root ganglion neurons.


Spinal Cord Dorsal Root Ganglion Schwann Cell Dorsal Root Ganglion Neuron Axonal Regeneration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Aebischer P, Guénard V, Winn SR, Valentini RF and Galletti PM (1988) Semi-permeable guidance channels allow peripheral nerve regeneration in the absence of a distal stump. Brain Res 454: 179–187Google Scholar
  2. 2.
    Aebischer P, Guénard V, and Brace S (1989) Peripheral nerve regeneration through semi-permeable guidance channels: effect of the molecular weight cut-off. J Neurosci 9: 3590–3595Google Scholar
  3. 3.
    Aebischer P, Guénard V and Valentini RF (1990) The morphology of regenerating peripheral nerves is modulated by the surface microgeometry of polymelic guidance channels. Brain Res 351:211–218Google Scholar
  4. 4.
    Aguayo A (1985) Axonal regeneration from injured neurons in the adult mammalian central nervous system. In: Synaptic Pasticity. Ed CW Cotman. New York: Guilford Press pp 457–484Google Scholar
  5. 5.
    Bunge MB, Paino, C and Fernandez-Valle C (1993) Implantation of cultured Schwann cells to foster repair in injured mammalian spional cord. In: Tissue Engineering. Current Perspectives. Ed. E Bell. Boston: Birkhauser. pp 48–57Google Scholar
  6. 6.
    Guénard V, Xu XM and Bunge MB (1993) The use of Schwann cell transplantation to foster central nervous system repair. Semin Neurosci 5: 401–411Google Scholar
  7. 7.
    Guénard V, Kleitman N, Morrissey TK, Bunge RP and Aebischer P (1992) Syngeneic Schwann cells derived from adult nerves seeded in semipermeable guidance channels enhance peripheral nerve regeneration. J Neurosci 12: 3310–3320Google Scholar
  8. 8.
    Paino CL and Bunge MB (1991). Induction of axon growth into Schwann cell implants grafted into lesioned adult rat spinal cord. Exper Neurol 114: 254–257Google Scholar
  9. 9.
    Paino CL, Fernandez-Valle C, Bates ML and Bunge MB (1994) Regrowth of axons in lesioned adult spional cord: promotion by implants of cultured Schwann cells. J Neurocytol 23: 433–452Google Scholar
  10. 10.
    Watson BD, Holets VR, Prado R and Bunge MB (1993) Laser-driven photochemical induction of spinal cord injury in the rat: methodology, histopathology and applications. Neuroprotocols 3: 3–15Google Scholar
  11. 11.
    Xu XM, Guénard V, Kleitman N and Bunge MB (1992) Axonal growth into Schwann cell-seeded guidance channels grafted into transected adult rat spinal cord. Soc Neurosci Abstr 18: 1479Google Scholar
  12. 12.
    Xu XM, Gubnard V, Chen A, Kleitman N and Bunge MB (1993) Rostral and caudal axonal regeneration into Schwann cell-seeded guidance channels grafted into a gap in adult rat spinal cord. Soc Neurosci Abstr 19: 681Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Mary Bartlett Bunge
    • 1
  1. 1.The Miami Project to Cure Paralysis and the Department of Cell Biology and AnatomyUniversity of Miami School of MedicineMiamiUSA

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