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Parasitology Research

, Volume 78, Issue 4, pp 298–302 | Cite as

Use of a recombinantDictyocaulus viviparus antigen in an enzyme-linked immunosorbent assay for immunodiagnosis of bovine dictyocaulosis

  • T. Schnieder
Article

Abstract

A specific recombinant antigen was evaluated for its immunodiagnostic potential in an enzyme-linked immunosorbent assay (ELISA). The antigen was used as a glutathione S-transferase (GST) fusion protein (DvGST3-14) or as a pure recombinant parasite protein (Dv3-14). A total of 55 sera from cattle experimentally infected withDictyocaulus viviparus, 24 sera from naturally infected cattle and 25 sera from helminth-free cattle were examined. ELISA results obtained for the sera from experimental infections showed a calculated specificity of >99% for both antigen preparations had a sensitivity of 93% (DvGST3-14) and 91% (Dv3-14). For field sera from natural infections, the specificity was calculated to be 90% (DvGST3-14) and >99% (Dv3-14) and the sensitivity, 85% (DvGST3-14) and 70% (Dv3-14).

Keywords

Glutathione Fusion Protein Immunosorbent Assay Experimental Infection Natural Infection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Baermann G (1917) Eine einfache Methode zur Auffingdung von Ankylostomum-(Nematoden)-Larven in Erdproben. Tijdschr Diergeneeskd 57:131–137Google Scholar
  2. Bellmer A (1990) Seroepidemiologische Untersuchungen über das Vorkommen vonDictyocaulus-Infektionen bei Rindern in Niedersachsen mit Hilfe des ELISA. Thesis, School of Veterinary Medicine, HannoverGoogle Scholar
  3. Boon JH, Kloostermann A, Brink R van den (1982) The incidence ofDictyocaulus viviparus infections in cattle in the Netherlands: I. The enzyme linked immunosorbent assay as a diagnostic tool. Vet Q 4:155–160PubMedGoogle Scholar
  4. Bos HJ, Beekman-Boneschanscher J, Boon JH (1986) Use of ELISA to assess lungworm infection in calves. Vet Rec 118:153–156Google Scholar
  5. De Leeuw WA, Cornelissen JBWJ (1991) Identification and isolation of a specific antigen with diagnostic potential fromDictyocaulus viviparus. Vet Parasitol 39:137–147PubMedGoogle Scholar
  6. Gasser RB, Lightowlers MW, Rickard MD (1990) A recombinant antigen with potential for serodiagnosis ofEchinococcus granulosus infection in dog. Int J Parasitol 20:943–950PubMedGoogle Scholar
  7. Griner PF, Mayewski RJ, Mushlin AI, Greenland P (1981) Selection and interpretation of diagnostic tests and procedures. Principles and applications. Ann Intern Med 94:553–600Google Scholar
  8. Kohler-Bellmer S (1991) Weitere Untersuchungen zum Einsatz des ELISA in Epizootiologie und Diagnostik beDictyocaulus viviparus im Landkreis Diepholz. Thesis, School of Veterinary Medicine, HannoverGoogle Scholar
  9. Mitchell GF (1989) Glutathione S-transferases-potential components of anti-schistosome vaccines? Parasitol Today 5:34–37PubMedGoogle Scholar
  10. Müller N, Gottstein B, Vogel M, Flury R, Seebeck T (1989) Application of a recombinantEchinococcus multilocularis antigen in an enzyme-linked immunosorbent assay for immunodiagnosis of human alveolar echinococcosis. Mol Biochem Parasitol 36:151–160PubMedGoogle Scholar
  11. Rode B, Jørgensen RJ (1989) Baermannization ofDictyocaulus spp. from faeces of cattle, sheep and donkeys. Vet Parasitol 30:101–109Google Scholar
  12. Smith DB, Corcoran LM (1990) Expression and purification of glutathione S-transferase fusion proteins. In: Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds) Current protocols in molecular biology, suppl 10, unit 16.7 Greene Publishing Associates/John Wiley & Sons, New YorkGoogle Scholar
  13. Smith DB, Johnson KS (1988) Single-step purification of polypeptides expressed inEscherichia coli as fusions with glutathione S-transferase. Gene 67:31–40PubMedGoogle Scholar
  14. Tenter AM, Johnson AM (1991) Recognition of recombinantToxoplasma gondii antigens by human sera in an ELISA. Parasitol Res 77:197–203PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • T. Schnieder
    • 1
  1. 1.Institute of ParasitologySchool of Veterinary Medicine HannoverHannover 71Germany

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