Parasitology Research

, Volume 78, Issue 4, pp 298–302 | Cite as

Use of a recombinantDictyocaulus viviparus antigen in an enzyme-linked immunosorbent assay for immunodiagnosis of bovine dictyocaulosis

  • T. Schnieder


A specific recombinant antigen was evaluated for its immunodiagnostic potential in an enzyme-linked immunosorbent assay (ELISA). The antigen was used as a glutathione S-transferase (GST) fusion protein (DvGST3-14) or as a pure recombinant parasite protein (Dv3-14). A total of 55 sera from cattle experimentally infected withDictyocaulus viviparus, 24 sera from naturally infected cattle and 25 sera from helminth-free cattle were examined. ELISA results obtained for the sera from experimental infections showed a calculated specificity of >99% for both antigen preparations had a sensitivity of 93% (DvGST3-14) and 91% (Dv3-14). For field sera from natural infections, the specificity was calculated to be 90% (DvGST3-14) and >99% (Dv3-14) and the sensitivity, 85% (DvGST3-14) and 70% (Dv3-14).


Glutathione Fusion Protein Immunosorbent Assay Experimental Infection Natural Infection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • T. Schnieder
    • 1
  1. 1.Institute of ParasitologySchool of Veterinary Medicine HannoverHannover 71Germany

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