Parasitology Research

, Volume 78, Issue 4, pp 298–302 | Cite as

Use of a recombinantDictyocaulus viviparus antigen in an enzyme-linked immunosorbent assay for immunodiagnosis of bovine dictyocaulosis

  • T. Schnieder
Article

Abstract

A specific recombinant antigen was evaluated for its immunodiagnostic potential in an enzyme-linked immunosorbent assay (ELISA). The antigen was used as a glutathione S-transferase (GST) fusion protein (DvGST3-14) or as a pure recombinant parasite protein (Dv3-14). A total of 55 sera from cattle experimentally infected withDictyocaulus viviparus, 24 sera from naturally infected cattle and 25 sera from helminth-free cattle were examined. ELISA results obtained for the sera from experimental infections showed a calculated specificity of >99% for both antigen preparations had a sensitivity of 93% (DvGST3-14) and 91% (Dv3-14). For field sera from natural infections, the specificity was calculated to be 90% (DvGST3-14) and >99% (Dv3-14) and the sensitivity, 85% (DvGST3-14) and 70% (Dv3-14).

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References

  1. Baermann G (1917) Eine einfache Methode zur Auffingdung von Ankylostomum-(Nematoden)-Larven in Erdproben. Tijdschr Diergeneeskd 57:131–137Google Scholar
  2. Bellmer A (1990) Seroepidemiologische Untersuchungen über das Vorkommen vonDictyocaulus-Infektionen bei Rindern in Niedersachsen mit Hilfe des ELISA. Thesis, School of Veterinary Medicine, HannoverGoogle Scholar
  3. Boon JH, Kloostermann A, Brink R van den (1982) The incidence ofDictyocaulus viviparus infections in cattle in the Netherlands: I. The enzyme linked immunosorbent assay as a diagnostic tool. Vet Q 4:155–160PubMedGoogle Scholar
  4. Bos HJ, Beekman-Boneschanscher J, Boon JH (1986) Use of ELISA to assess lungworm infection in calves. Vet Rec 118:153–156Google Scholar
  5. De Leeuw WA, Cornelissen JBWJ (1991) Identification and isolation of a specific antigen with diagnostic potential fromDictyocaulus viviparus. Vet Parasitol 39:137–147PubMedGoogle Scholar
  6. Gasser RB, Lightowlers MW, Rickard MD (1990) A recombinant antigen with potential for serodiagnosis ofEchinococcus granulosus infection in dog. Int J Parasitol 20:943–950PubMedGoogle Scholar
  7. Griner PF, Mayewski RJ, Mushlin AI, Greenland P (1981) Selection and interpretation of diagnostic tests and procedures. Principles and applications. Ann Intern Med 94:553–600Google Scholar
  8. Kohler-Bellmer S (1991) Weitere Untersuchungen zum Einsatz des ELISA in Epizootiologie und Diagnostik beDictyocaulus viviparus im Landkreis Diepholz. Thesis, School of Veterinary Medicine, HannoverGoogle Scholar
  9. Mitchell GF (1989) Glutathione S-transferases-potential components of anti-schistosome vaccines? Parasitol Today 5:34–37PubMedGoogle Scholar
  10. Müller N, Gottstein B, Vogel M, Flury R, Seebeck T (1989) Application of a recombinantEchinococcus multilocularis antigen in an enzyme-linked immunosorbent assay for immunodiagnosis of human alveolar echinococcosis. Mol Biochem Parasitol 36:151–160PubMedGoogle Scholar
  11. Rode B, Jørgensen RJ (1989) Baermannization ofDictyocaulus spp. from faeces of cattle, sheep and donkeys. Vet Parasitol 30:101–109Google Scholar
  12. Smith DB, Corcoran LM (1990) Expression and purification of glutathione S-transferase fusion proteins. In: Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds) Current protocols in molecular biology, suppl 10, unit 16.7 Greene Publishing Associates/John Wiley & Sons, New YorkGoogle Scholar
  13. Smith DB, Johnson KS (1988) Single-step purification of polypeptides expressed inEscherichia coli as fusions with glutathione S-transferase. Gene 67:31–40PubMedGoogle Scholar
  14. Tenter AM, Johnson AM (1991) Recognition of recombinantToxoplasma gondii antigens by human sera in an ELISA. Parasitol Res 77:197–203PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • T. Schnieder
    • 1
  1. 1.Institute of ParasitologySchool of Veterinary Medicine HannoverHannover 71Germany

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