Plant Systematics and Evolution

, Volume 160, Issue 1–2, pp 29–37

Ribosomal RNA sequences for inferring phylogeny within the grass family (Poaceae)

  • R. Keith Hamby
  • Elizabeth A. Zimmer

DOI: 10.1007/BF00936707

Cite this article as:
Hamby, R.K. & Zimmer, E.A. Pl Syst Evol (1988) 160: 29. doi:10.1007/BF00936707


We have isolated RNA from nine different grass species and fromPsilotum, a modern representative of a primitive land plant lineage. By direct RNA sequencing with reverse transcriptase, we have determined the nucleotide sequence for five regions of the 18 S rRNA molecule and three regions of the 26 S rRNA molecule. Over 1 600 positions have been elucidated for each plant species. These sequences were aligned by computer and the variable positions were identified by inspection. The data from the variable positions were input into phylogenetic inference computer programs to generate an evolutionary relationship among the grass species. This evolutionary tree based on nucleotide sequence data was compared to a recent classification of thePoaceae based on morphological data.

Key words

Angiosperms Poaceae Evolution phylogenetic trees 18 S rRNA 26 S rRNA direct rRNA sequencing 

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • R. Keith Hamby
    • 1
  • Elizabeth A. Zimmer
    • 1
  1. 1.Departments of Biochemistry and BotanyLouisiana State University and the Louisiana Agricultural Experiment Station, Louisiana Agricultural CenterBaton RougeUSA

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