Plant Systematics and Evolution

, Volume 158, Issue 2–4, pp 97–106 | Cite as

Cytochemical heterochromatin differentiation inSinapis alba (Cruciferae) using a simple air-drying technique for producing chromosome spreads

  • Günter Geber
  • Dieter Schweizer


The fluorochrome and Giemsa chromosome banding patterns and the Ag-NOR histochemical staining ofSinapis alba are described. Two major types of heterochromatin can be distinguished, one of which contains GC-rich DNA. The observations are discussed as they relate to the known satellite DNAs ofS. alba. — A simple air-drying technique for producing spreads of plant mitotic chromosomes is presented. Different materials and staining techniques were tested showing that the method has wide applications.

Key words

Angiosperms Cruciferae Sinapis alba Chromosome banding chromosome spreading method 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ambros, P. F., Matzke, M. A., Matzke, A. J. M., 1986: Detection of a 17kb unique sequence (T-DNA) in plant chromosomes by in situ hybridization. — Chromosoma94, 11–18.Google Scholar
  2. Bennett, M. D., Smith, J. B., 1976: Nuclear DNA amounts in angiosperms. — Phil. Trans. R. Soc. Lond.B 274, 227–274.Google Scholar
  3. —, 1982: Nuclear DNA amounts in angiosperms. — Proc. Roy. Soc. Lond.B 216, 179–199.Google Scholar
  4. Capesius, I., 1979: Isolation and characterization of satellite DNA from mustard seedlings. — Pl. Syst. Evol.133, 1–13.Google Scholar
  5. —, 1983: Sequence of the cryptic satellite DNA from the plantSinapis alba. — Biochem. Biophys. Acta739, 276–280.Google Scholar
  6. —, 1974: Endopolyploidisierung während des Streckungswachstums der Hypokotyle vonSinapis alba. — Protoplasma82, 147–153.Google Scholar
  7. Grellet, F., Delcasso, D., Panabieres, F., Delseny, M., 1976: Organisation and evolution of a higher plant alphoid-like satellite DNA sequence. — J. Mol. Biol.187, 495–507.Google Scholar
  8. Halldén, C., Bryngelsson, T., Säll, T., Gustafsson, M., 1987: Distribution and evolution of a tandemly repeated DNA sequence in the familyBrassicaceae. — J. Mol. Evol.25, 318–323.Google Scholar
  9. Hizume, M., Sato, S., Tanaka, A., 1980: A highly reproducible method of nucleolus organizing regions staining in plants. — Stain Technol.55, 87–90.Google Scholar
  10. Hsu, T. C., 1952: Mammalian chromosomes in vitro. I. The karyotype of man. — J. Heredity43, 167–172.Google Scholar
  11. Kodama, Y., Yoshida, M. C., Sasaki, M., 1980: An improved silver staining technique for nucleolus organizer regions by using nylon cloth. — Japan. J. Human. Genet.25, 229–233.Google Scholar
  12. Moyzis, R. K., Albright, K. L., Bartholdi, M. F., Cram, L. S., Deaven, L. L., Hildebrand, C. E., Joste, N. E., Longmire, J. L., Meyne, J., Schwarzacher-Robinson, T., 1987: Human chromosome-specific repetitive DNA sequences: Novel markers for genetic analysis. — Chromosoma95, 375–386.Google Scholar
  13. Murata, M., 1983: Staining air dried protoplasts for study of plant chromosomes. — Stain Technol.58, 101–106.Google Scholar
  14. Nagl, W., 1982: Cell growth and nuclear DNA increase by endoreduplication and differential DNA replication. — InNicolini, C., (Ed.): Cell Growth. New York, London: Plenum press. fluorescent staining of heterochromatic regions in human chromosomes 9, 15, and the Y by D 287/170. — Hum. Genet.59, 10–13.Google Scholar
  15. Schwarzacher, T., Ambros, P., Schweizer, D., 1980: Application of Giemsa banding to orchid karyotype analysis. — Pl. Syst. Evol.134, 293–297.Google Scholar
  16. Schweizer, D., 1981: Counterstain-enhanced chromosome banding. — Hum. Genet.57, 1–14.Google Scholar
  17. -Ambros, P., Gründler, P., Varga, F., 1987: Attempts to relate cytological and molecular chromosome data ofArabidopsis thaliana to its genetic linkage map. — Arabid. Inf. Serv. (in press).Google Scholar
  18. Tschermak-Woess, E., 1963: Strukturtypen der Ruhekerne von Pflanzen und Tieren. — Protoplasmatologia, Handbuch der Protoplasmaforschung V. — Wien: Springer.Google Scholar
  19. —, 1953: Veränderungen der Kernstruktur während der Endomitose, rhythmisches Kernwachstum und verschiedenes Heterochromatin bei Angiospermen. — Chromosoma5, 574–614.Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Günter Geber
    • 1
  • Dieter Schweizer
    • 1
  1. 1.Institute of BotanyUniversity of ViennaViennaAustria

Personalised recommendations