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Cytochemical heterochromatin differentiation inSinapis alba (Cruciferae) using a simple air-drying technique for producing chromosome spreads

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Abstract

The fluorochrome and Giemsa chromosome banding patterns and the Ag-NOR histochemical staining ofSinapis alba are described. Two major types of heterochromatin can be distinguished, one of which contains GC-rich DNA. The observations are discussed as they relate to the known satellite DNAs ofS. alba. — A simple air-drying technique for producing spreads of plant mitotic chromosomes is presented. Different materials and staining techniques were tested showing that the method has wide applications.

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Dedicated in gratitude to Prof. DrElisabeth Tschermak-Woess on the occasion of her 70th birthday.

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Geber, G., Schweizer, D. Cytochemical heterochromatin differentiation inSinapis alba (Cruciferae) using a simple air-drying technique for producing chromosome spreads. Pl Syst Evol 158, 97–106 (1988). https://doi.org/10.1007/BF00936336

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Key words

  • Angiosperms
  • Cruciferae
  • Sinapis alba
  • Chromosome banding
  • chromosome spreading method