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Electron microscope studies on the development of the external zone of the mouse median eminence

  • Laila Eurenius
  • Rune Jarskär
Article

Summary

The development of the external zone of the median eminence of the mouse was studied in the electron microscope. The examination follows the development of the embryo from the 15th day of the gestation period and the juvenile growth until 24 days of age.

Single terminals of the tubero-infundibular neurons of the external zone were found to extend to the outer basement membrane of the perivascular space of the portal primary capillary plexus in the 16 day-old embryo. In the 18 day-old embryo a narrow external zone has developed. Organization of the external zone into the adult pattern is accomplished at the age of three to four weeks. Small agranular as well as large granular vesicles are present in the tubero-infundibular nerve terminals even in the 16 day-old embryo.

Changes in the organization of the nerve endings along the outer perivascular basement membrane in relation to the ependymal vascular feet were considered.

Key-Words

Median eminence Mouse Growth and development Ultrastructure 

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References

  1. Björklund, A., Enemar, A., Falck, B.: Monoamines in the hypothalamo-hypophyseal system of the mouse with special reference to the ontogenetic aspects. Z. Zellforsch.89, 590–607 (1968).PubMedGoogle Scholar
  2. —, Falck, B., Hromek, F., Owman, C., West, K. A.: Identification and terminal distribution of the tubero-hypophyseal monoamine fibre systems in the rat by means of stereotaxic and microspectroflourimetric techniques. Brain Res.17, 1–24 (1970).PubMedGoogle Scholar
  3. Bock, R., aus der Mühlen, K.: Beiträge zur funktionellen Morphologie der Neurohypophyse. I. Über eine “gomori-positive” Substanz in der Zona externa infundibuli beidseitig adrenalektomierter weißer Mäuse. Z. Zellforsch.92, 130–148 (1968).PubMedGoogle Scholar
  4. Clementi, F., Ceccarelli, B., Cerati, E., Demonte, M. L., Felici, M., Motta, M., Pecile, A.: Subcellular localization of neurotransmitters and releasing factors in the rat median eminence. J. Endocr.48, 205–213 (1970).PubMedGoogle Scholar
  5. Corrodi, H., Jonsson, G.: The formaldehyde fluorescence method for the histological demonstration of biogenic amines. A review on the methodology. J. Histochem. Cytochem.15, 65–78 (1967).Google Scholar
  6. Daikoku, S., Sato, T. J. A., Hashimoto, T., Moroshita, H.: Development of the ultrastructures of the median eminence and supraoptic nuclei in rats. Tokushima J. exp. Med.15, 1–15 (1968).PubMedGoogle Scholar
  7. Duffy, P. E., Menefee, M.: Electron microscopic observations of neurosecretory granules, nerve and glial fibers and blood vessels in the median eminence of the rabbit. Amer. J. Anat.117, 251–286 (1965).PubMedGoogle Scholar
  8. Enemar, A.: The structure and development of the hypophysial portal system in the laboratory mouse, with particular regard to the primary plexus. Ark. Zool., II Ser.13, 203–252 (1961a).Google Scholar
  9. - Notes on the histogenesis of the hypophysis of the laboratory mouse, with special reference to its relation to the development of the hypophysial portal system. Kungl. Fysiogr. Sällsk. Handl., N. F.71, Nr.19 (1961b).Google Scholar
  10. Eränkö, O.: The practical histochemical demonstration of catecholamines by formaldehyde-induced fluorescence. J. roy. micr. Soc.87, 259–276 (1967).PubMedGoogle Scholar
  11. Falck, B.: Cellular localization of monoamines. Progr. Brain Res.8, 28–44 (1964).Google Scholar
  12. —, Hillarp, N.- Å., Thieme, G., Torp, A.: Fluorescence of catecholamines and related compounds condensed with formaldehyde. J. Histochem. Cytochem.10, 348–354 (1962).Google Scholar
  13. Fuxe, K.: Cellular localization of monoamines in the median eminence and infundibular stem of some mammals. Z. Zellforsch.61, 710–724 (1964).PubMedGoogle Scholar
  14. —, Hökfelt, T.: Further evidence for the existence of tubero-infundibular dopamine neurons. Acta physiol. scand.66, 245–246 (1966).PubMedGoogle Scholar
  15. — —: Catecholamines in the hypothalamus and the pituitary gland. In: Frontiers in neuroendocrinology, p. 47–96, ed. by W. F. Ganong and L. Martini. New York-London-Toronto: Oxford University Press 1969.Google Scholar
  16. — —: Participation of central monoamine neurons in the regulation of anterior pituitary function with special regard to the neuroendocrine role of tubero-infundibular dopamine neurons. In: Aspects of neuroendocrinology, p. 192–205, eds. W. Bargmann, B. Scharrer. Berlin-Heidelberg-New York: Springer 1970.Google Scholar
  17. Ganong, W. F., Lorentzén, L.: Brain neurohumors and endocrine function. In: Neuroendocrinology, vol.2, p. 583–640, ed. by L. Martini and W. F. Ganong. New York-London: Academic Press 1967.Google Scholar
  18. Hökfelt, T.: In vitro studies on central and peripheral monoamine neurons at the ultrastructural level. Z. Zellforsch.91, 1–74 (1968).PubMedGoogle Scholar
  19. Hökfelt, T.: Electron microscopic studies on peripheral and central monoamine neurons. In: Aspects of neuroendocrinology, p. 79–95, eds. W. Bargmann, B. Scharrer. Berlin-Heidelberg-New York: Springer 1970.Google Scholar
  20. Hyyppä, M.: A histochemical study of the primary catecholamines in the hypothalamic neurons of the rat in relation to the ontogenetic and sexual differentiation. Z. Zellforsch.98, 550–561 (1969).PubMedGoogle Scholar
  21. Kobayashi, H., Oota, Y., Uemura, H., Hirano, T.: Electron microscopic and pharmacologic studies on the rat median eminence. Z. Zellforsch.71, 387–404 (1966).Google Scholar
  22. Kobayashi, T., Kobayashi, T., Yamamoto, K., Kaibara, M., Ajika, K.: Electron microscopic observation on the hypothalamo-hypophyseal system in rats. IV. Ultrafine structure of the developing median eminence. Endocr. jap.15, 337–363 (1968).Google Scholar
  23. — — — — —: Electron microscopic observations of axonal inclusions in rat median eminence. Endocr. jap., Suppl.1, 11–27 (1969).Google Scholar
  24. Lichtensteiger, W., Langemann, H.: Uptake of exogenous catecholamines by monoamine-containing neurons of the central nervous system: uptake of catecholamines by arcuato-infundibular neurons. J. Pharmacol. exp. Ther.151, 400–408 (1966).PubMedGoogle Scholar
  25. Loizou, L. A.: The postnatal development of monamine-containing structures in the hypothalamo-hypophyseal system of the albino rat. Z. Zellforsch.114, 234–253 (1971).PubMedGoogle Scholar
  26. Matsui, T.: Fine structure of the median eminence of the rat. J. Fac. Sci. Univ. Tokyo4, 71–96 (1966).Google Scholar
  27. —: Effect of reserpine on the distribution of granulated vesicles in the mouse median eminence. Neuroendocrinology2, 99–106 (1967).Google Scholar
  28. Mazzuca, M.: Structure fine de l'éminence médiane du cobaye. J. Microscop.4, 225–238 (1965).Google Scholar
  29. Monroe, B. G.: A comparative study of the ultrastructure of the median eminence, infundibular stem and neural lobe of the hypophysis of the rat. Z. Zellforsch.76, 405–432 (1967).PubMedGoogle Scholar
  30. Mulder, A. H., Geuze, J. J., Wied, D., de: Studies on the subcellular localization of corticotropin releasing factor (CRF) and vasopressin in the median eminence of the rat. Endocrinology87, 61–80 (1970).PubMedGoogle Scholar
  31. Odake, G.: Fluorescence microscopy of the catecholamine-containing neurons of the hypothalamo-hypophyseal system. Z. Zellforsch.82, 46–64 (1967).PubMedGoogle Scholar
  32. Oota, Y.: Fine structure of the median eminence and pars nervosa of the mouse. J. Fac. Sci. Univ. Tokyo10, 155–168 (1963).Google Scholar
  33. Richardson, K. C.: Electron microscopic identification of autonomic nerve endings. Nature (Lond.)210, 756 (1966).Google Scholar
  34. Rinne, U. K.: Ultrastructure of the median eminence of the rat. Z. Zellforsch.74, 98–122 (1966).PubMedGoogle Scholar
  35. —, Arstila, A. U.: Ultrastructure of the neurovascular link between the hypothalamus and the anterior pituitary gland in the median eminence of the rat. Neuroendocrinology1, 214–227 (1966a).Google Scholar
  36. — —: Electron microscopic evidence on the significance of the granular and vesicular inclusions of the neurosecretory nerve endings in the median eminence of the rat. I. Ultrastructural alterations after reserpine injection. Med. Pharmacol. exp.15, 357–369 (1966b).Google Scholar
  37. Röhlich, P., Vigh, B., Teichmann, I., Aros, B.: Electron microscopy of the median eminence of the rat. Acta biol. Acad. Sci. hung.15, 431–457 (1965).PubMedGoogle Scholar
  38. Sano, Y., Odake, G., Takemoto, S.: Fluorescence microscopic and electron microscopic observations on the tuberohypophyseal tract. Neuroendocrinology2, 30–42 (1967).Google Scholar
  39. Scott, D. E., Knigge, K. M.: Ultrastructural changes in the median eminence of the rat following deafferentation of the basal hypothalamus. Z. Zellforsch.105, 1–33 (1970).PubMedGoogle Scholar
  40. Smith, G. C., Simpson, R. W.: Monoamine fluorescence in the median eminence of foetal, neonatal and adult rats. Z. Zellforsch.104, 541–557 (1970).PubMedGoogle Scholar
  41. Szentágothai, J., Flerkó, B., Mess, B., Halász, B.: Hypothalamic control of the anterior pituitary. Budapest: Akadémiai Kiadó 1962.Google Scholar
  42. Zambrano, D.: On the presence of neurons with granulated vesicles in the median eminence of rat and dog. Neuroendocrinology3, 141–155 (1968).PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1971

Authors and Affiliations

  • Laila Eurenius
    • 1
  • Rune Jarskär
    • 1
  1. 1.Department of ZoologyUniversity of GothenburgGothenburg 33Sweden

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