Molecular and Cellular Biochemistry

, Volume 145, Issue 2, pp 185–187 | Cite as

Rapid isolation of any known genes from whole cells of yeast by PCR

  • Dindial Ramotar
Short Communication

Abstract

A short protocol was developed that allows the rapid isolation of any knownSaccharomyces cerevisiae gene. Two known genes,APN1 andIMP2, were isolated directly from whole cells of yeast using polymerase chain reaction, without the need for purified template genomic DNA.

Key words

yeast polymerase chain reaction gene method 

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References

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    Donnini C, Lodi T, Ferrero I, Puglisi PP:IMP2, A nuclear gene controlling the mitochondrial dependence of galactose, maltose, and raffinose utilization inSaccharomyces cerevisiae. Yeast 8: 83–83, 1992Google Scholar

Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Dindial Ramotar
    • 1
  1. 1.Environment and HealthCHUL Research CentreSte-FoyCanada

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