Parasitology Research

, Volume 77, Issue 2, pp 152–156

Schistosomin, a peptide present in the haemolymph ofLymnaea stagnalis infected withTrichobilharzia ocellata, is produced only in the snail's central nervous system

  • H. D. F. H. Schallig
  • P. L. Hordijk
  • P. W. Oosthoek
  • M. de Jong-Brink
Original Investigations

Abstract

A peptide, schistosomin, is present in haemolymph ofLymnaea stagnalis infected withTrichobilharzia ocellata. There are indications that schistosomin is produced by the central nervous system (CNS) of the snail. Schistomin inhibits the effects of the snail's gonadotropic hormones, e.g. calfluxin (CaFl). CaFl stimulates Ca2+ influx into the mitochondria of the albumen gland, as shown using the ultracytochemical potassium pyroantimonate precipitation technique. We investigated the question as to whether schistosomin is produced only by the snail or by both the snail and the parasite. Several types of extract of stages of the parasite and of the CNS of the snail were tested for their capability to inhibit the CaFl response. Acid extracts of cercariae and of CNS showed an inhibitory effect, suggesting that both contain schistosomin. From these extracts, material was obtained that showed the same HPLC characteristics as schistosomin. This material was tested again. The hormone response was inhibited only by material derived from the CNS of the snail, indicating that the parasite does not produce schistosomin.

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References

  1. Bliss CI (1967) Statistics in biology, vol 1. McGraw-Hill, New YorkGoogle Scholar
  2. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72:248–254Google Scholar
  3. Dresden MH, Asch HL (1972) Proteolytic enzymes in extracts ofSchistosoma mansoni cercariae. Biochim Biophys Acta 289:378–384Google Scholar
  4. Dictus WJAG, Ebberink RHM (1988) Structure of one of the neuropeptides of the egg-laying hormone precursor ofLymnaea. Mol Cell Endocrinol 60:23–29Google Scholar
  5. Dictus WJAG, Jong-Brink M de, Boer HH (1987) A neuropeptide (calfluxin) is involved in the influx of calcium into the mitochondria of the albumen gland of the freshwater snailLymnaea stagnalis. Gen Comp Endocrinol 65:439–450Google Scholar
  6. Geraerts WPM, Leeuwen JPT van, Nuyt K, De With ND (1981) Cardioactive peptides of the CNS of the pulmonate snailLymnaea stagnalis. Experientia 37:1168–1169Google Scholar
  7. Hordijk PL, Ebberink RHM, Jong-Brink M de, Joosse J (1989) Receptor-mediated inhibition of reproductive activity in a schistosome-infected freshwater snail. In: Evangelopoulos AE, Changeux JP, Packer L, Sotiroudis TG, Wirts KWA (eds) Receptors, membrane transport and signal transduction. NATO ASI Ser H: Cell Biol 29:372–381Google Scholar
  8. Jong-Brink M de, Bergamin-Sassen MJM (1989)Trichobilharzia ocellata: influence of infection on the interaction between the dorsal body hormone, a female gonadotropic hormone, and the follicle cells in the gonad of the intermediate snail hostLymnaea stagnalis. Exp Parasitol 68:93–98Google Scholar
  9. Jong-Brink M de, Elsaadany MM (1987) Schistosomin, a parasitic factor interfering with reproduction inLymnaea stagnalis. In: Boer HH, Geraerts WPM, Joosse J (eds) Neurobiology molluscan models. North Holland, Amsterdam, pp 150–155Google Scholar
  10. Jong-Brink M de, Elsaadany MM, Boer HH (1988a)Trichobilharzia ocellata: interference with the endocrine control of female reproduction of its hostLymnaea stagnalis. Exp Parasitol 65:91–100Google Scholar
  11. Jong-Brink M de, Elsaadany MM, Boer HH (1988b) Schistosomin, an antagonist of calfluxin. Exp Parasitol 65:109–118Google Scholar
  12. Joosse J, Elk R van (1986)Trichobilharzia ocellata: physiological characterization of giant growth, glycogen depletion and absence of reproductive activity in the intermediate host snail,Lymnaea stagnalis. Exp Parasitol 62:1–13Google Scholar
  13. Joosse J, Elk R van, Mosselman S, Wortelboer HM, Diepen JCE van (1988) Schistosomin: a pronase-sensitive agent in the haemolymph ofTrichobilharzia ocellata—infectedLymnaea stagnalis inhibits the activity of albumen glands in vitro. Parasitol Res 74:228–234Google Scholar
  14. McClelland G, Bourns TKR (1969) Effects ofTrichobilharzia ocellata on growth, reproduction and survival ofLymnaea stagnalis. Exp Parasitol 24:137–146Google Scholar
  15. Mellink JJ, Bovenkamp W van den (1985) In vitro culture of intramolluscan stages of the avian schistosomeTrichobilharzia ocellata. Z Parasitenkd 71:337–351Google Scholar
  16. Meuleman EA (1972) Host-parasite interrelationships between the freshwater pulmonateBiomphalaria pfeifferi and the trematodeSchistoma mansoni. Neth J Zool 22:355–427Google Scholar
  17. Meulerman EA, Huyer AR, Mooij JH (1984) Maintenance of the life cycle ofTrichobilharzia ocellata via the duckAnas platyrrhynchos and the pond snailLymnaea stagnalis. Neth J Zool 34:414–417Google Scholar
  18. Mueller JF (1974) The biology ofSpirometra. J Parasitol 60:3–14Google Scholar
  19. Pan CT (1965) Studies on the host-parasite relationship betweenSchistosoma mansoni and the snailAustralorbis glabrata. Am J Trop Med Hyg 14:931–976Google Scholar
  20. Ramaley JA, Phares CK (1980) Delay of puberty onset in females due to suppression of growth hormone. Endocrinology 106:1989–1993Google Scholar
  21. Salem MAM, Phares CK (1986) Some biochemical effects of the growth hormone analogue produced by plerocercoids of the tapewormSpirometra mansonoides on carbohydrate metabolism of adipose tissue from normal, diabetic and hypophysectomized rats. J Parasitol 72:498–502Google Scholar
  22. Schallig HDFH, Schut A, Van der Knaap WPW, Jong-Brink M de (1990) A simplified medium for the in vitro culture of mother sporocysts of the schistosomeTrichobilharzia ocellata. Parasitol Res 76:278–279Google Scholar
  23. Shapiro SS, Wilk MB (1965) An analysis of variance test for normality. Biometrika 52:591–611Google Scholar
  24. Slocum RD, Roux JR (1982) An improved method for the subcellular localization of calcium using a modification of the antimonate precipitation technique. J Histochem Cytochem 30:617–629Google Scholar
  25. Sluiters JF (1981) Development ofTrichobilharzia ocellata inLymnaea stagnalis and the effects of infection on the reproductive system of the host. Z Parasitenkd 64:303–319Google Scholar
  26. Sluiters JF, Dogterom GE (1984) The effect of infection ofLymnaea stagnalis withTrichobilharzia ocelleta on the presence of an reactivity to the ovulation hormone of the host. Z Parasitenkd 70:485–489Google Scholar
  27. Sluiters JF, Brussaard-Wüst CM, Meuleman EA (1980) The relationship between miracidial dose, production of cercariae and reproductive activity of the host in the combination ofTrichobilharzia ocellata andLymnaea stagnalis. Z Parasitenkd 63:13–26Google Scholar
  28. Sluiters JF, Roubos EW, Joosse J (1984) Increased activity of the female gonadotropic hormone-producing dorsal bodies inLymnaea stagnalis infected withTrichobilharzia ocellata. Z Parasitenkd 70:67–72Google Scholar
  29. Sokal RR, Rohlf FJ (1981) Biometry: the principles and practice of statistics in biological research. Freeman and Company. San FranciscoGoogle Scholar
  30. Steen WJ van der, Hoven NP van den, Jager JC (1969) A method for breeding and studying freshwater snails under continous water change with some references on growth and reproduction inLymnaea stagnalis. Neth J Zool 19:131–139Google Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • H. D. F. H. Schallig
    • 1
  • P. L. Hordijk
    • 1
  • P. W. Oosthoek
    • 1
  • M. de Jong-Brink
    • 1
  1. 1.Faculty of BiologyVrije UniversiteitAmsterdamThe Netherlands

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