Archives of Gynecology and Obstetrics

, Volume 246, Issue 3, pp 181–187 | Cite as

Detection of early pregnancy factor (EPF) in pregnant and nonpregnant subjects with the rosette inhibition test

  • W. Straube
  • U. Tiemann
  • M. Loh
  • M. Schütz
Originals

Summary

We tested for early pregnancy factor (EPF) using a rosette inhibition test with polyclonal anti-lymphocyte serum from the horse and two monoclonal antibodies specific for the E-receptor of T-lymphocytes. When lymphocytes were preincubated with early human pregnancy sera, rosette inhibition titres were four or more dilutions higher (P<0.01) than when lymphocytes were preincubated with nonpregnant sera.

Key words

Early pregnancy factor Rosette inhibition test Polyclonal anti-lymphocyte serum Monoclonal antibody 

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References

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • W. Straube
    • 2
  • U. Tiemann
    • 1
  • M. Loh
    • 2
  • M. Schütz
    • 2
  1. 1.Forschungszentrum für Tierproduktion Dummerstorf-Rostock der Akademie der Landwirtschaftswissenschaft der DDRGDR
  2. 2.Klinik für Gynäkologie und Geburtshilfe der Ernst-Moritz-Arndt-UniversitätGreifswald

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