Parasitology Research

, Volume 80, Issue 6, pp 474–477 | Cite as

Intra- and interspecific polymorphisms ofLeishmania donovani andL. tropica minicircle DNA

  • P. Dobner
  • T. Löscher
  • H. Rinder
Original Paper

Abstract

A pair of degenerate polymerase chain reaction (PCR) primers (LEI-1, TCG GAT CC[C,T] [G,C]TG GGT AGG GGC GT; LEI-2, ACG GAT CC[G,C] [G,C][A,C]C TAT [A,T]TT ACA CC) defining a 0.15-kb segment ofLeishmania minicircle DNA was constructed. These primers amplified not only inter- but also intraspecifically polymorphic sequences. Individual sequences revealed a higher intraspecific than interspecific divergence. It is concluded that individual sequences are of limited relevance for species determination. In contrast, when a data base of 19 different sequences was analyzed in a dendrographic plot, an accurate species differentiation was feasible.

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References

  1. Barker DC, Gibson LJ, Kennedy WPK, Nasser AAAA, Williams RH (1986) The potential of using recombinant DNA species-specific probes for the identification of tropicalLeishmania. Parasitology 91:S139-S174Google Scholar
  2. Blackwell JM (1992) Leishmaniasis epidemiology: all down to the DNA. Parasitology 104:S19-S34PubMedGoogle Scholar
  3. Bruijn MHL de, Barker DC (1992) Diagnosis of New World leishmaniasis: specific detection of species of theLeishmania braziliensis complex by amplification of kinetoplast DNA. Acta Trop (Basel) 52:45–58Google Scholar
  4. Higgins DG, Sharp PM (1988) CLUSTAL: a package for performing multiple sequence alignment on a microcomputer. Gene 73:237–244PubMedGoogle Scholar
  5. Kidane GZ, Hughes D, Simpson L (1984) Sequence heterogeneity and anomalous electrophoretic mobility of kinetoplast minicircle DNA fromLeishmania tarentolae. Gene 27: 265–277PubMedGoogle Scholar
  6. Kreutzer RD, Grogl M, Neva FA, Fryauff DJ, Magill AJ, Aleman-Munoz MM (1993) Identification and genetic comparison of leishmanial parasites causing viscerotropic and cutaneous disease in soldiers returning from operation Desert Storm. Am J Trop Med Hyg 49:357–363PubMedGoogle Scholar
  7. Laskay T, Kiessling R, Rinke DeWit TF, Wirth DF (1991) Generation of species-specific DNA probes forLeishmania aethiopica. Mol Biochem Parasitol 44:279–286PubMedGoogle Scholar
  8. Lee S, Tarn C, Chang K (1993) Characterization of the switch of kinetoplast DNA minicircle dominance during development and reversion of drug resistance inLeishmania. Mol Biochem Parasitol 58:187–204PubMedGoogle Scholar
  9. Magill AJ, Grögl M, Gasser RA, Sun W, Oster CN (1993) Visceral infection caused byLeishmania tropica in veterans of operation Desert Storm. N Engl J Med 328:1383–1387PubMedGoogle Scholar
  10. Rogers WO, Wirth DF (1988) Generation of sequence diversity in the kinetoplast DNA minicircles ofLeishmania mexicana amazonensis. Mol Biochem Parasitol 30:1–8PubMedGoogle Scholar
  11. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467PubMedGoogle Scholar
  12. Smith DF, Searle S, Ready PD, Gramiccia M, Ben-Ismail R (1989) A kinetoplast DNA probe diagnostic forLeishmania major: sequence homologies between regions ofLeishmania minicircles. Mol Biochem Parasitol 37:213–224PubMedGoogle Scholar
  13. Sommer R, Tautz D (1989) Minimal homology requirements for PCR primers. Nucl Acids Res 17:6749PubMedGoogle Scholar
  14. Stuart K (1983) Kinetoplast DNA, mitochondrial DNA with a difference. Mol Biochem Parasitol 9:93–104PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • P. Dobner
    • 1
  • T. Löscher
    • 1
  • H. Rinder
    • 1
  1. 1.Abteilung für Infektions- und TropenmedizinUniversität MünchenMünchenGermany

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