Parasitology Research

, Volume 80, Issue 1, pp 16–21 | Cite as

Demonstration of heat-shock protein 70 in the sporozoite stage of malaria parasites

  • Moriya Tsuji
  • Denise Mattei
  • Ruth S. Nussenzweig
  • Daniel Eichinger
  • Fidel Zavala
Original Paper


Three monoclonal antibodies generated by immunization of mice withPlasmodium berghei-infected red blood cells were found to react with the 75-kDa heat-shock protein (HSP70) present in liver stages and crythrocytic forms of the parasites. These antibodies were shown to react with a recombinant protein encoding the carboxyl terminal half of PfHSP70 (aa 365–681). Differently from earlier results, we clearly demonstrated that HSP70 was also expressed in the sporozoite stage, using these monoclonal antibodies in an immunofluorescence and Western immunoblot assay. These monoclonal antibodies react not only with sporozoites ofP. berghei, the parasites originally used for the immunization, but also with sporozoites of several other rodent and human plasmodial species. Passive transfer of these monoclonal antibodies into naive mice, simultaneously injected with sporozoites, failed to neutralize the infectivity ofP. berghei sporozoites and to inhibit the development of liver stages ofP. yoelii.


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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Moriya Tsuji
    • 1
  • Denise Mattei
    • 3
  • Ruth S. Nussenzweig
    • 1
  • Daniel Eichinger
    • 2
  • Fidel Zavala
    • 1
  1. 1.Department of Medical and Molecular ParasitologyNew York University School of MedicineNew YorkUSA
  2. 2.Division of Immunology, Department of PathologyNew York University School of MedicineNew YorkUSA
  3. 3.Unité de Parasitologie ExperimentaleInstitut PasteurParis Cedex 15France

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