Parasitology Research

, Volume 80, Issue 5, pp 381–387

Echinococcus multilocularis: In vitro interactions between protoscolices and Kupffer cells

  • S. Walbaum
  • S. al Nahhas
  • C. Gabrion
  • M. Mesnil
  • A. F. Petavy
Original Paper

DOI: 10.1007/BF00932375

Cite this article as:
Walbaum, S., al Nahhas, S., Gabrion, C. et al. Parasitol Res (1994) 80: 381. doi:10.1007/BF00932375

Abstract

Echinococcus multilocularis protoscolices collected from experimentally infected jirds were incubated for 2 weeks in rat hepatic cell cultures cocultivated with or without feeder cells (BALB/c 3T3 and IAR 20). Scanning and transmission electron microscopy studies were performed during the course of the culture period. Kupffer cells (Kc) were seen adhering to the anterior and posterior ends of the protoscolices. Some protoscolices were fixed to the cell monolayer by a cluster of Kc adhering to the posterior end of the parasite. These cells were phagocytosing the glycocalyx and the electrondense distal end of the microthrix of the protoscolex tegument. An alteration in the superficial tegumental cytoplasm with extensive mitochondrial damage was also noted. The properties expressed by Kc against protoscolices in vitro might also be relevant for proliferation of metacestodes in vivo.

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • S. Walbaum
    • 1
  • S. al Nahhas
    • 1
  • C. Gabrion
    • 2
  • M. Mesnil
    • 3
  • A. F. Petavy
    • 1
  1. 1.INSERM Département de ParasitologieUniversité Claude BernardLyon Cedex 08France
  2. 2.Laboratoire de Parasitologie ComparéeURA 698 CNRS USTLMontpellier CedexFrance
  3. 3.Centre International de Recherche sur le CancerLyon Cedex 08France

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