Parasitology Research

, Volume 80, Issue 5, pp 366–373 | Cite as

The genome ofEimeria tenella: Further studies on its molecular organisation

  • M. W. Shirley
Original Paper

Abstract

The molecular karyotype of the coccidial parasiteEimeria tenella has been further resolved. Different conditions of pulsed-field gel electrophoresis were used to separate up to 14 chromosomes that ranged in size between 1 and in excess of 6 megabase pairs (Mb) of DNA. Comparisons of several populations revealed some size polymorphisms of homologous chromosomes. Probes containing tandem repeats of the DNA sequence CAG and the telomeric-like sequence TTTAGGG hybridised to each chromosome. All other probes containing either repetitive or non-repetitive sequences were mapped to individual chromosomes.

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References

  1. Canning EU, Anwar M (1968) Studies on meiotic division in coccidial and malarial parasites. J Protozool 15:290–298Google Scholar
  2. Chapman HD, Rose ME (1986) Cloning ofEimeria tenella in the chicken. J Parasitol 72:605–606Google Scholar
  3. Corcoran LM, Thompson JK, Walliker D, Kemp DJ (1988) Homologous recombination within subtelomeric repeat sequences generates chromosome size polymorphisms inPlasmodium falciparum. Cell 53:807–813Google Scholar
  4. Cornelissen AWCA, Overdulve JP, Van der Ploeg M (1984) Determination of nuclear DNA of five eucoccidian parasites,Isospora (Toxoplasma) gondii, Sarcocystis cruzi, Eimeria tenella, E. acervulina and Plasmodium berghei, with special reference to gametogenesis and meiosis inI. (T.) gondii. Parasitology 88:521–553Google Scholar
  5. Cowman AF, Lew AM (1990) Chromosomal rearrangements and point mutations in the DHFR-TS gene ofPlasmodium chabaudi under antifolate selection. Mol Biochem Parasitol 42:21–30Google Scholar
  6. Fernando MA, Pasternak JJ (1991)Eimeria spp. of the domestic fowl: resolution of chromosomes by field inversion gel electrophoresis. Exp Parasitol 72:306–310Google Scholar
  7. Foote SJ, Thompson JK, Cowman AF, Kemp DJ (1989) Amplification of the multi-drug resistance gene in some chloroquine resistant isolates ofPlasmodium falciparum. Cell 57:921–930Google Scholar
  8. Kemp DJ, Thompson JK, Walliker D, Corcoran LM (1987) Molecular karyotype ofPlasmodium falciparum: conserved linkage groups and expendable histidine-rich protein genes. Proc Natl Acad Sci USA 84:7672–7676Google Scholar
  9. Kim K, Gooze L, Petersen C, Gut J, Nelson RG (1992) Isolation, sequence and molecular karyotype analysis of the actin gene ofCryptosporidium parvum. Mol Biochem Parasitol 50:105–114Google Scholar
  10. Ko C, Smith CK, McDonell M (1990) Identification and characterization of a target antigen of a monoclonal antibody directed againstEimeria tenella merozoites. Mol Biochem Parasitol 41:53–64Google Scholar
  11. Liberator PA, Hsu J, Turner MJ (1989) Tandem trinucleotide repeats throughout the nucleotide sequence of a cDNA encoding anEimeria tenella sporozoite antigen. Nucleic Acids Res 17:7401Google Scholar
  12. Long PL (1972)Eimeria tenella: reproduction, pathogenicity and immunogenicity of a strain maintained in chicken embryos by serial passage. J Comp Pathol 82:439–445Google Scholar
  13. Mead JR, Arrowood MJ, Current WL, Sterling CR (1988) Field inversion gel electrophoretic separation ofCryptosporidium spp. chromosome-sized DNA. J Parasitol 74:366–369Google Scholar
  14. Mencher D, Sar-Shalom O, Pillimer G, Halabi A, Wallach M (1989)Eimeria maxima sexual stage antigens: cloning and expression of a high molecular weight gametocyte antigen. In: Yvore P (ed) Coccidia and intestinal coccidiomorphs. Proceedings of the Vth International Coccidiosis Conference, Tours, France. INRA, Paris, pp 605–608Google Scholar
  15. McDonald VM, Shirley MW, Millard BJ (1986) A comparative study of two lines ofEimeria tenella attenuated either by selection for precocious development in the chicken or by growth in chicken embryos. Avian Pathol 15:323–335Google Scholar
  16. McDougald LR, Jeffers TK (1976)Eimeria tenella (Sporozoa, Coccidia): gametogony following a single asexual generation. Science 192:258–259Google Scholar
  17. Morzaria SP, Spooner PR, Bishop RP, Musoke AJ, Young JR (1990) SfiI and NotI polymorphisms inTheileria stocks detected by pulsed field gel electrophoresis. Mol Biochem Parasitol 40:203–212Google Scholar
  18. Pologe LG, Ravetch JV (1988) Large deletions result from breakage and healing ofPlasmodium falciparum chromosomes. Cell 55:869–874Google Scholar
  19. Ponzi M, Pace T, Dore E, Frontali C (1985) Identification of a telomeric DNA sequence inPlasmodium berghei. EMBO J 4:2991–2995Google Scholar
  20. Richards EJ, Ausubel FM (1988) Isolation of a higher eucaryotic telomere fromArabidopsis thaliana. Cell 53:127–136Google Scholar
  21. Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N (1985) Enzymatic amplification of B-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anaemia. Science 230:1350–1354Google Scholar
  22. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New YorkGoogle Scholar
  23. Schmatz DM, Crane St J, Murray PK (1984) Purification ofEimeria sporozoites by DE-52 anion exchange chromatography. J Protozool 31:181–183Google Scholar
  24. Scholtyseck E (1963) Untersuchungen über die Kernverhältnisse und das Wachstum bei Coccidiomorphen unter besonderer Berücksichtigung vonEimeria maxima. Z Parasitenkd 22:428–474Google Scholar
  25. Shirley MW (1975) Enzyme variation inEimeria species of the chicken. Parasitology 71:369–376Google Scholar
  26. Shirley MW (1994) Coccidial parasites from the chicken: discrimination of different populations ofEimeria tenella by DNA hybridisation. Res Vet Sci (in press)Google Scholar
  27. Shirley MW, Millard BJ (1976) Some observations on the sexual differentiation ofEimeria tenella using single sporozoite infections in chicken embryos. Parasitology 73:337–341Google Scholar
  28. Shirley MW, Kemp DJ, Pallister J, Prowse SJ (1990) A molecular karyotype ofEimeria tenella as revealed by contour-clamped homogenous electric field gel electrophoresis. Mol Biochem Parasitol 38:169–174Google Scholar
  29. Sibley LD, Boothroyd JC (1992) Construction of a molecular karyotype forToxoplasma gondii. Mol Biochem Parasitol 51:291–300Google Scholar
  30. Sibley LD, LeBlanc AJ, Pfefferkorn ER, Boothroyd JC (1992) Generation of a restriction fragment length polymorphism linkage map forToxoplasma gondii. Genetics 132:1003–1015Google Scholar
  31. Stucki U, Braun R, Roditi I (1993)Eimeria tenella: characterization of a 5s ribosomal RNA repeat unit and its use as a species-specific probe. Exp Parasitol 76:68–75Google Scholar
  32. Su H-Y, Tenter AM, Johnson AM (1992) Genomic analysis ofSarcocystis by contour-clamped homogeneous electric fields. J Protozool Res 2:16–24Google Scholar

Copyright information

© Springer Verlag 1994

Authors and Affiliations

  • M. W. Shirley
    • 1
  1. 1.Compton LaboratoryAFRC Institute for Animal HealthNr NewburyUK

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