An electron immunogold-labeling technique was used in conjunction with a post-embedding procedure to demonstrate for the first time the ultrastructural distribution of the parasitic platyhelminth neuropeptide, neuropeptide F (NPF(, in the nervous system of the cestodeMoniezia expansa. Two axon types, distinguished by their populations of different-sized electron-dense vesicles, were identified. Immunogold labeling demonstrated an apparent homogeneity of PP, FMR Famide and NPF (M. expansa) antigenic sites throughout the larger dense-cored vesicles within the central nervous system. Triple labeling clearly demonstrated the co-localisation of immunoreactivities (IR) for NPF, PP and FMR Famide within the same dense-cored vesicles. The presence of NPF-IR within the vesicles occupying the perikaryon of the neuronal cell body indicated that the peptides had undergone post-translational C-terminal amidation prior to entering the axon. Antigen pre-absorption experiments using NPF prevented labeling with either PP or FMR famide antisera, and the failure of these antisera to block NPF-IR supports the view that some, if not all, of the PP/FMR Famide-IR is due to NPF-like peptides.
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Brennan GP, Halton DW, Maule AG, Shaw C, Johnston CF, Moore S, Fairweather I (1993) Immunoelectron microscopical studies of regulatory peptides in the nervous system of the monogenean parasite,Diclidophora merlangi. Parasitology 106 171–176
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Brennan, G.P., Halton, D.W., Maule, A.G. et al. Electron immunogold labeling of regulatory peptide immunoreactivity in the nervous system ofMoniezia expansa (Cestoda: Cyclophyllidea). Parasitol Res 79, 409–415 (1993). https://doi.org/10.1007/BF00931831
- Neuronal Cell
- Cell Body
- Regulatory Peptide
- Neuronal Cell Body