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Parasitology Research

, Volume 81, Issue 6, pp 465–469 | Cite as

Ultrastructure ofBlastocystis hominis cysts

  • V. Zaman
  • J. Howe
  • M. Ng
Original Paper

Abstract

Blastocystis hominis cysts were concentrated from human faeces by repeated washing in distilled water and centrifugation on Ficoll-Paque. This procedure gave a concentrated suspension of cysts without yielding any non-cystic forms. The cysts were examined by both transmission (TEM) and scanning electron microscopy (SEM). Cysts were surrounded by a fibrillar layer that appeared porous in SEM. Many naked cysts without the fibrillar layer were also visible in SEM. They were variable in shape but mostly circular to oval. The diameter of the cyst without the fibrillar layer was 3.5 μm in both TEM and SEM. The nuclear structure was typical ofBlastocystis and exhibited multiple mitochondria with poorly developed cristae. Glycogen was present in small to large clumps in the cytoplasm. The cyst wall was 5–100 nm thick and was bounded internally by an electron-dense plasmalemma.

Keywords

Electron Microscopy Scanning Electron Microscopy Nuclear Structure Cyst Wall Concentrate Suspension 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • V. Zaman
    • 1
  • J. Howe
    • 2
  • M. Ng
    • 2
  1. 1.Department of MicrobiologyAga Khan UniversityKarachiPakistan
  2. 2.Department of MicrobiologyNational University of SingaporeSingapore

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