Burgia malayi andB. pahangi microfilariae were isolated from the blood of infectedMastomys natalensis, and were exsheathed by freezing, thawing and agitation. Pure sheaths were obtained by a filtration procedure. The sheaths were found to contain about 95 mol% of amino acids, with proline, glutamic acid/glutamine, alanine, cysteine/cystine and glycine being the major components, and 5 mol% of carbohydrates, notably (N-acetyl)galactosamine, but no (N-acetyl)glucosamine.
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Klonisch, T., Bardehle, G., Linder, D. et al. The sheaths ofBrugia microfilariae: Isolation and composition. Parasitol Res 77, 448–451 (1991). https://doi.org/10.1007/BF00931643