Parasitology Research

, Volume 81, Issue 2, pp 158–162 | Cite as

Cyclic nucleotide changes induced in human leukocytes by a product of axenically grownEntamoeba histolytica that inhibits human monocyte locomotion

  • G. Rico
  • O. Díaz-Guerra
  • R. R. Kretschmer
Original Paper


Pulse exposure of human mononuclear phagocytes to the monocyte locomotion-inhibitory factor produced byEntamoeba histolytica (i.e., the 369- to 765-Da chromatographic fraction obtained from the supernatant fluid of axenically grownE. histolytica) led to a swift increase in the intracellular concentration of adenosine 3′:5′ cyclic monophosphate (cAMP). A weaker response was observed in human polymorphonuclear leukocytes, the locomotion of which, however, is not inhibited by this amebic factor. The same chromatographic fraction obtained from the axenic medium control lacked this effect, at least upon mononuclear phagocytes. On the other hand, both the monocyte locomotion-inhibitory factor and the axenic medium control, possibly through shared culture medium components, induced comparable increases in guanosine 3′:5′ cyclic monophosphate (cGMP) in human mononuclear phagocytes and in polymorphonuclear leukocytes, thus suggesting that the latter nucleotide is not critical for the leukotactic inhibitory phenomenon. Our results suggest that like other leukotactic inhibitors, the monocyte locomotion-inhibitory factor produced byE. histolytica operates through modulations of intracellular cAMP.


Guanosine Human Monocyte Cyclic Nucleotide Polymorphonuclear Leukocyte Culture Medium Component 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Mononuclear phagocytes (monocytes)


monocyte locomotion-inhibitory factor


polymorphonuclear leukocytes


adenosine 3′:5′ cyclic monophosphate


guanosine 3′:5′ cyclic monophosphate


phosphate-buffered (pH 7.4) normal saline


axenic medium control Gey's-A; Gey's medium with 2% albumin


zymosan-activated serum


high-power field (400x)


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Copyright information

© Springer Verlag 1995

Authors and Affiliations

  • G. Rico
    • 1
  • O. Díaz-Guerra
    • 1
  • R. R. Kretschmer
    • 1
  1. 1.Unidad de Immunología, Subjefatura de Investigación Biomédica, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social and Subdivisión de Medicina Experimental, Facultad de MedicinaUniversidad Nacional Autónoma de MéxicoMéxicoMéxico

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