Parasitology Research

, Volume 81, Issue 2, pp 148–151 | Cite as

Entamoeba histolytica autochthonous isolates from mentally retarded Italian patients

  • S. Gatti
  • C. Cevini
  • L. Marchi
  • S. Novati
  • M. Scaglia
Original Paper


A total of 77 mentally retarde male inpatients residing in a psychiatric institution in northern Italy were screened for the presence of stool parasites,Entamoeba histolytica particularly. Parasitological stool examination showedEntamoeba spp. (E. histolytica and/orE. dispar) in 26 cases (33.7%). In vitro culture on Robinson's medium was positive in 16 cases (61.1%); in 11 cases we could stabilize and clone the isolates and proceed to electrophoretic assays. In all cases, patterns of pathogenic zymodemes were found (zymodeme II, 3 isolates; zymodeme XII, 4 isolates; zymodeme XIV, 4 isolates). All isolates were therefore identified asE. histolytica.


Italian Patient Psychiatric Institution Entamoeba Histolytica Stool Examination Male Inpatient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Braun TI, Fekete T, Lynch A (1988) Strongyloidiasis in an institution for mentally retarded adults. Arch Intern Med 148:634–636Google Scholar
  2. Brumpt E (1925) Etude sommaire de l'“Entamoeba dispar” n.sp. Amibe a kystes quadrinucleés, parasite de l'homme. Bull Acad Natl Med (Paris) 94:934–952Google Scholar
  3. Choudhuri G, Prakash V, Kumar A, Kumar Shahi S, Sharma M (1991) Protective immunity toEntamoeba histolytica infection in subjects with antiamoebic antibodies residing in a hyperendemic zone. Scand J Infect Dis 23:771–776Google Scholar
  4. Diamond LS, Clarck CG (1993) A redescription ofEntamoeba histolytica Schaudinn, 1903 (emended Walker, 1911) separating it fromEntamoeba dispar Brumpt, 1925. J Euk Mikrobiol 40:340–344Google Scholar
  5. Gonzalez A, Aguirre A, Guhl F, Marinkelle CJ, Gottstein B, Warhurst DC, Miles MA (1991) Development and pilot evaluation of an immunodiagnostic test for invasive amebiasis (abstract). Trans R Soc Trop Med Hyg 85:312Google Scholar
  6. Gonzalez-Ruiz A, Haque R, Rehmant T, Aguirre A, Jaramillo C, Castanon G, Hall A, Guhl F, Ruiz-Palacios G, Warhurst DC, Miles MA (1992) A monoclonal antibody for distinction of invasive and noninvasive clinical isolates ofEntamoeba histolytica. J Clin Microbiol 30:2807–2813Google Scholar
  7. Jackson TFHG, Gathiram V, Simjee AE (1985) Seroepidemiological study of antibody responses to the zymodemes ofEntamoeba histolytica. Lancet: 716–719Google Scholar
  8. Kaneda Y, Nagakura K, Tachibana H, Tanaka T (1988)Entamoeba histolytica infection in a rehabilitation center for mentally retarded persons in Japan. Scand J Infect Dis 20:687Google Scholar
  9. Nagakura K, Tachibana H, Tanaka T, Kaneda Y, Tojunaga M, Sasao M, Takeuchi T (1989) An outbreak of amoebiasis in an institution for mentally retarded. Jpn J Med Sci Biol 42:63–67Google Scholar
  10. Nagakura K, Tachibana H, Kaneda Y, Suzuki H, Sasaoka K, Kobayashi S, Takeuchi T (1990) Amebiasis in institutions for the mentally retarded in Kanagawa prefecture. Japan. Jpn J Med Sci Biol 43:123–131Google Scholar
  11. Petri WA Jr, Ravdin JI (1988) Amebiasis in institutionalized populations. In: Ravdin JI (ed) Amebiasis. Human infection byEntamoeba histolytica. Churchill Livingstone, New York, pp 576–581Google Scholar
  12. Robinson GL (1968) The laboratory diagnosis of human parasitic amoebae. Trans R Soc Trop Med Hyg 62:285–294Google Scholar
  13. Sargeaunt PG (1987) The reliability ofEntamoeba histolytica zymodemes in clinical diagnosis. Parasitol Today 3:40–43Google Scholar
  14. Sargeaunt PG (1992)Entamoeba histolytica is a complex of two species. Austr Microbiol 13:359–361Google Scholar
  15. Sargeaunt PG, Williams JE (1978) Electrophoretic isoenzyme patterms ofEntamoeba histolytica andEntamoeba coli. Trans R Soc Trop Med Hyg. 72:164–166Google Scholar
  16. Sargeaunt PG, Williams JE (1988) A study of intestinal protozoa including non-pathogenicEntamoeba histolytica from patients in a group of mental hospitals. Am J Public Health 72:178–180Google Scholar
  17. Scaglia M, Gatti S, Bruno A, Cevini C, Marchi L, Sargeaunt PG (1991) Autochthonous amoebiasis in institutionalized mentally-retarded patients: preliminary evaluation of isoenzyme patterns in three isolates. Ann Trop Med Parasitol 85:509–513Google Scholar
  18. Schaudinn F (1903) Untersuchungen über die Fortpflanzung einiger Rhizopoden. (Vorläufige Mitteilung). Arb Kaiserl Gesundheitsamte 19:547–576Google Scholar
  19. Sexton PG, Krogstad DJ, Spencer HC Jr, Healy GR, Sinclair S, Sledge CE, Shultz MG (1974) Amoebiasis in a mental institution: serologic and epidemiologic studies. Am J Epidemiol 100:414–423Google Scholar
  20. Tannich E, Burchard GD (1991) Differentiation of pathogenic from non-pathogenicEntamoeba histolytica by restriction fragment analysis of a single gene amplified in vitro. J Microbiol 29:250–255Google Scholar
  21. Tannich E, Horstmann RD, Knobloch J, Arnold HH (1989) Genomic DNA differences between pathogenic and non-pathogenicEntamoeba histolytica. Proc Natl Acad Sci USA 86:5118–5122Google Scholar
  22. Thacker SB, Simpson S, Gordon TJ, Wolfe M, Kimball AM (1979) Parasitic disease control in a residential facility for the mentally retarded. Am J Public Health 9:1279–1281Google Scholar

Copyright information

© Springer Verlag 1995

Authors and Affiliations

  • S. Gatti
    • 1
  • C. Cevini
    • 1
  • L. Marchi
    • 2
  • S. Novati
    • 1
  • M. Scaglia
    • 1
  1. 1.Laboratory of Clinical Parasitology, Institute of Infectious DiseasesUniversity-IRCCSPaviaItaly
  2. 2.Division of Infectious DiseasesCremona HospitalCremonaItaly

Personalised recommendations