Molecular and Cellular Biochemistry

, Volume 136, Issue 1, pp 23–28 | Cite as

Insulin stimulates turnover of phosphatidylcholine in rat adipocytes

  • S. Lance Macaulay
  • Richard G. Larkins


The present study investigated the effect of insulin on phosphatidylcholine turnover in rat adipocytes labelled to equilibrium with [14C]-choline. Insulin induced a rapid turnover of this major phospholipid that was maximal by 1 min and transient in nature. Following a 1 min stimulation of the cells with insulin at a maximally effective concentration (7 nM), a 4–6% decrease in the percentage of total cellular choline associated with this phospholipid was observed. This reflected a significant transient increase in the percentage of total cellular choline associated with phosphorylcholine, which together with diacylglycerol are the phospholipase C cleavage products of phosphatidylcholine. These effects were observed over a physiological range of insulin concentrations. No effect of insulin on any other choline phospholipid or metabolite (sphingomyelin, lysophophatidylcholine, glycerophosphocholine or choline) was seen. These results suggest that insulin stimulates a phospholipase C-mediated turnover of phosphatidylcholine in rat adipocytes. The rapid nature of this turnover suggests a potential role in signal transduction.

Key words

phosphatidylcholine phospholipase C insulin cell signalling (rat adipocytes) 


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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • S. Lance Macaulay
    • 1
    • 2
  • Richard G. Larkins
    • 2
  1. 1.CSIRO Division of Biomolecular EngineeringParkvilleAustralia
  2. 2.Department of Medicine, Royal Melbourne HospitalUniversity of MelbourneParkvilleAustralia

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