The expression of bradyzoite-specific antigens (Bsa) ofToxoplasma gondii was studied in murine bonemarrow-derived macrophages that had previously been infected with tachyzoites. Growth conditions that allowed only restricted replication ofToxoplasma gondii resulted in heterogeneous populations; (1) Bsa-positive and Bsa-negative parasites could be observed within one parasitophorous vacuole (heterogeneous vacuole community), and (2) homogeneous Bsa-positive and homogeneous Bsa-negative vacuole communities coexisted within one macrophage host cell. These observations suggest that stage conversion does not seem to be a synchromous event for a vacuole community.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Adams WB, Hibbs Jr JB, Taintor RR, Krahenbuhl JL (1990) Microbiostatic effect of murine-activated macrophages forToxoplasma gondii. J Immunol 144:2725–2729
Bohne W, Heesemann J, Gross U (1993) Induction of bradyzoitespecificToxoplasma gondii antigens in gamma interferon-treated mouse macrophages. Infect Immun 61:1141–1145
Flesch IEA, Kaufmann SHE (1991) Mechanisms involved in mycobacterial growth inhibition by gamma interferon-activated bone marrow macrophages: role of reactive nitrogen intermediates Infect Immun 59:3213–3218
Gross U, Müller WA, Knapp S, Heesemann J (1991) Identification of a virulence-associated antigen ofToxoplasma gondii by a mouse monoclonal antibody. Infect Immun 59:4511–4516
Langermans JAM, Hulst MEB van der, Nibbering PH, Hiemstra PS, Fransen L, Furth R van (1992) IFN-γ-induced-l-argininedependent toxoplasmastatic activity in murine peritoneal macrophages is mediated by endogenous tumor necrosis factor-α. J Immunol 148:568–574
Suzuki Y, Orellana MA, Schreiber RD, Remington JS (1988) Interferon-γ: the major mediator of resistance againstToxoplasma gondii. Science 240:516–518
Tomavo S, Fortier B, Soete M, Ansel C, Camus D, Dubremetz JF (1991) Characterization of bradyzoite-specific antigens ofToxoplasma gondii. Infect Immun 59:3750–3753
About this article
Cite this article
Bohne, W., Heesemann, J. & Gross, U. Coexistence of heterogeneous populations ofToxoplasma gondii parasites within parasitophorous vacuoles of murine macrophages as revealed by a bradyzoite-specific monoclonal antibody. Parasitol Res 79, 485–487 (1993). https://doi.org/10.1007/BF00931588
- Monoclonal Antibody
- Growth Condition
- Host Cell
- Heterogeneous Population
- Murine Macrophage