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Glucantime susceptibility ofLeishmania promastigotes under variable growth conditions

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Growth inhibition ofLeishmania promastigotes by glucantime was compared in three different media. Glucantime inhibited the growth ofLeishmania cultured in complex medium but did not affect parasite growth when added to cells cultured in defined or semidefined media. Supplementation of the complex medium with biopterin partially reversed the glucantime effect in sensitive strains, although the addition of folic acid or oleic acid did not alter the activity of glucantime. Differences in fatty acid composition were observed between strains showing different degrees of glucantime susceptibility.

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  1. Bannon CD, Craske JD, Hai NT, Harper NL O'Rourke KL (1982) Analysis of fatty acid methyl esters with accuracy and reliability. II. Methylation of fats and oils with boron trifluoride-methanol. J Chromatogr 247: 63–69

  2. Beck JT, Ullman B (1991) Biopterin conversion to reduced folates byLeishmania donovani promastigotes. Mol Biochem Parasitol 49: 21–28

  3. Berman JD, Grogl M (1988)Leishmania mexicana: chemistry and biochemistry of sodium stribogluconate (pentostam). Exp Parasitol 67: 96–103

  4. Berman JD, Waddel D, Hanson BD (1985) Biochemical mechanisms of the antileishmanial activity of sodium stibogluconate. Antimicrob Agents Chemother 27: 916–920

  5. Berman JD, Edwards N, Kink M, Grogl M (1989) Biochemistry of pentostam resistantLeishmania. Am J Trop Med Hyg 40: 159–164

  6. Croft SL (1986) In vitro screens in the experimental chemotherapy of leishmaniasis and trypanosomiasis. Parasitol Today 2: 64–69

  7. Deane MP, Souza MA, Pereira NM, Gonçalves AM, Momen H, Morel CM (1984)Trypanosoma cruzi: inoculation schedules and reisolation methods select individual strains from doubly infected mice as demonstrated by schizodeme analysis. J Protozool 31: 276–280

  8. Dewey VC, Kidder GW (1966) Effects of long-chain mono- and dicarboxylic acids on the pteridine requirement ofCrithidia. Arch Biochem Biophys 115: 401–406

  9. Figueiredo Y, Costa CA, Mayrink W, Araujo FG, Dias M, Melo MN, Magalhães P, Willians P, Batista SM, Coelho MV (1976) Nutrição e metabolismo de formas de cultura deLeishmania. Rev Inst Med Trop Sao Paulo 18: 306–314

  10. Fish WR, Holz GG Jr, Beach DH, Owen E, Anekwe GE (1981) The cyclopropane fatty acid of trypanosomatids. Mol Biochem Parasitol 3: 103–115

  11. Forrest HS, Van Baalen C (1970) Microbiology of unconjugated pteridines. Annu Rev Microbiol 24: 91–108

  12. Grimaldi G Jr, McMahon-Pratt D (1991) Leishmaniasis and its etiologic agents in the New World. An overview. In: Sum T (ed) Progress in clinical parasitology, ch 3. Field and Wood, Philadelphia, pp 73–118

  13. Grogl M, Oduola AMJ, Cordero LDC, Kyle DE (1989)Leishmania spp.: development of pentostam-resistant clones in vitro by discontinuous drug exposure. Exp Parasitol 69: 78–90

  14. Hakomori SI, Murakami WT (1988) Glycolipids of hamster fibroblasts and derived malignant-transformed cell lines. Proc Natl Acad Sci USA 59: 254–261

  15. Hunter KJ, Strobos CAM, Fairlamb AH (1991) Inhibition of polyamine biosynthesis inCrithidia fasciculata byD, L-alpha-difluoromethylornithine andD,L-alpha-difluoromethylarginine. Mol Biochem Parasitol 46: 35–44

  16. Kaur K, Emmett K, McCann PP, Sjoerdsma A, Ullman B (1986) Effects ofD,L-alpha-difluoromethylornithine onLeishmania donovani promastigotes. J Protozool 33: 518–521

  17. Melo MN, Azevedo HP, Roitman I, Mayrink W (1985) A new defined medium for cultivatingLeishmania promastigotes. Acta Trop (Basel) 42: 137–141

  18. Moreira ESA (1988) Effect of meglumina antimoniate (glucan-time) and verapamil onLeishmania promastigotes. Mem Inst Oswaldo Cruz 83 [Suppl]: 182

  19. Moreira ESA, Petrillo-Peixoto ML (1991) In vitro activity of meglumine antimoniate, a pentavalent antimonial drug, onLeishmania promastigotes. Braz J Med Biol Res 24: 459–469

  20. Mottram JC, Coombs GH (1985)Leishmania mexicana: enzyme activities of amastigotes and promastigotes and their inhibition by antimonials and arsenicals. Exp Parasitol 59: 151–160

  21. Nilson O, Svernnerholm L (1982) Characterization and quantitative determination of gangliosides and neutral glycosphingo-lipids in human liver. J Lipid Res 23: 327–334

  22. Nolan TJ, Herman R (1985) Effects of long-term in vitro cultivation onLeishmania donovani promastigotes. J Protozool 32: 70–75

  23. Pacheco RS, Grimaldi G, Momen H, Morel CM (1990) Population heterogeneity among clones of New WorldLeishmania species. Parasitology 100: 393–398

  24. Petrillo-Peixoto ML, Beverley SM (1987) In vitro activity of sulfonamides and sulfones againstLeishmania major promastigotes. Antimicrob Agents Chemother 31: 1575–1578

  25. Sadigursky M, Brodskyn CI (1986) A new liquid medium without blood and serum for culture of hemoflagellates. Am J Trop Med Hyg 35: 942–944

  26. Scott DA, Coombs GH, Sanderson BE (1987) Folate ultilization byLeishmania species and the identification of intracellular derivatives and folate-metabolising enzymes. Mol Biochem Parasitol 23: 139–149

  27. Singer SJ (1974) The molecular organization of membranes. Annu Rev Biochem 43: 805–833

  28. Ullman B, Carrero-Valenzuela E, Coons T (1989)Leishmania donovani: isolation and characterization of sodium stiboglucanate (pentostam)-resistant cell lines. Exp Parasitol 69: 157–163

  29. Yorke W, Murgatroyd F (1930) Studies in chemotherapy. III. The action in vitro of certain arsenical and antimonials compounds onTrypanosoma rhodesiense and on atoxyl and acriflavine-resistant strains of the parasite. Ann Trop Med Parasitol 24: 449–476

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Correspondence to Elizabeth Spangler.

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Spangler, E., de Araujo Soares, A.M.R.M. & de Lourdes Petrillo-Peixoto, M. Glucantime susceptibility ofLeishmania promastigotes under variable growth conditions. Parasitol Res 81, 291–295 (1995). https://doi.org/10.1007/BF00931532

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  • Acid Composition
  • Folic Acid
  • Growth Condition
  • Oleic Acid
  • Fatty Acid Composition