Abstract
The authors previously reported that an extract fromZingiber officinale, traditionally eaten along with raw fish and used in traditional Chinese medicine, effectively destroyedAnisakis larvae in vitro. In this study, we analyzed the effective components of ginger rhizomes. Methanol extracts were fractionated after first being treated with HCl at pH 3, then with NaHCO3 at pH 10, and, finally, with NaOH at pH 13 (fraction 1). In general, this fraction is rich in neutral substances. [6]-Shogaol and [6]-gingerol, known neutral components of ginger rhizomes, were detected using gas chromatography and were found to be the most prevalent components in the fraction, occurring in quantities that resulted in a dose-dependent killing efficacy. Authentic [6]-shogaol and [6]-gingerol could killAnisakis larvae at a minimal effective dose of 62.5 and 250 μg/ml, respectively. However, the concentration of [6]-gingerol in fraction 1 was >20 times that of [6]-shogaol, making the former the most active component in the fraction. Furthermore, synergistic effects between [6]-gingerol and a small amount of [6]-shogaol were observed. Pyrantel pamoate, an available antinematodal drug, had no lethal effect, even at a concentration of 1 mg/ml. In saline solution containing [6]-shogaol (62.5 μg/ml), >90% of larvae lost spontaneous movement within 4 h and were destroyed completely within 16 h. Microscopical examinations showed destruction of the digestive tract and disturbances of cuticulae.
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References
Akamatu K (1980) Kyoh Wakanyaku, Ishiyaku shuppan, Tokyo (in Japanese)
Connell DW, Sutherland MD (1969) A reexamination of gingerol, shogaol and zingerone, the pungent principles of ginger (Zingiber officinale). Aust J Chem 252:1033–1043
Eschenmoser A, Schinz H (1950) Zur Kenntnis der Sesquiterpene und Azulene Konstitution des Zingibere. Helv Chim Acta 33:171–177
Ishikura H, Kobayashi Y, Miyamoto K, Yagi K, Nakajima O, Fujita O, Oikawa Y, Maejima J, Aji T, Akao Y, Hayasaka H (1988) Transition of occurrence of anisakiasis and its paratenic host fishes in Japan, with pathogenesis of anisakiasis. Hokkaido J Med Sci 63:376–391
Iwano H, Ishikura H, Hayasaka N (1974) A study on prophylaxis of anisakiasis: 3. Effect of pyrantel pamoate. Hokkaido J Surg 19:83–90
Kasuya S, Goto C, Ohotomo H (1988) Studies on prophylaxis againstAnisakis — a screening of killing effects of extract from foods on the larvae. Jpn Assoc Infect Dis 62:1152–1156
Kasuya S, Goto C, Koga K, Ohotomo H, Kagei N (1990) Lethal efficacy of leaf extract fromPerilla frutescens (traditional Chinese medicine) or perillaldehyde onAnisakis larvae in vitro. Jpn J Parasitol 39:220–225
Nakamura H, Yamamoto T (1982) Mutagen and anti-mutagen in ginger,Zingiber officinale. Mutat Res 103:119–126
Narasimhan S, Govindarajan VS (1978) Evalution of spices and oleoresin: VI. Pungency of the ginger components gingerol and shogaol and their quality. J Food Technol 13:31–36
Schantz PM (1989) The dangers of eating raw fish. N Engl J Med 320:1143–1145
Suekawa M, Ishige A, Yuasa K, Sudo K, Aburada M, Hosoya E (1984) Pharmacological studies on ginger I. Pharmacological actions of pungent constituents, [6]-gingerol and [6]-shogaol. J Pharmacobiodyn 7:836–848
Tanaka J, Torisu M (1978)Anisakis and eosinophil: I. Detection of a soluble factor selectively chemotactic for eosinophils in the extract fromAnisakis larvae. J Immunol 120:745–749
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Goto, C., Kasuya, S., Koga, K. et al. Lethal efficacy of extract fromZingiber officinale (traditional Chinese medicine) or [6]-shogaol and [6]-gingerol inAnisakis larvae in vitro. Parasitol Res 76, 653–656 (1990). https://doi.org/10.1007/BF00931082
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DOI: https://doi.org/10.1007/BF00931082