Advertisement

Parasitology Research

, Volume 76, Issue 3, pp 199–206 | Cite as

Sexual cycle ofBabesia divergens confirmed by DNA measurements

  • Ute Mackenstedt
  • Martina Gauer
  • Heinz Mehlhorn
  • Eberhard Schein
  • Susann Hauschild
Original Investigations

Abstract

The DNA content of the developmental stages within the life cycle ofBabesia divergens was measured by means of fluorescence microscopy using the DNA-specific bisbenzimide Hoechst 33258. By comparing the mean relative fluorescence intensities, the assumption of sexual reproduction in the gut of the tick vector (Ixodes ricinus) was confirmed. However, no proof of a pre- or postzygotic meiosis was found.

Keywords

Life Cycle Fluorescence Intensity Developmental Stage Fluorescence Microscopy Sexual Reproduction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

E

erythrocyte

G

gamont

GA

gamete

K

kinete

ME

merozoite

N

nucleus

R

ring stage (binucleated)

RC

reticulocyte

SB

sporoblast

SP

sporozoite

ST

Strahlenkörper

YR

young ring stage (trophozoite; uninucleated)

YZ

young zygote

Z

zygote

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Frerichs WM, Holbrook AA (1974) Feeding mechanisms ofBabesia equi. J Protozool 21:707–709Google Scholar
  2. Friedhoff KT (1981) Morphologic aspects ofBabesia in the tick. In: Ristic M, Kreier JP (eds) Babesiosis. Academic, New York, pp 143–169Google Scholar
  3. Friedhoff KT, Büscher G (1976) Rediscovery of Koch's Strahlenkörper ofBabesia bigemina. Z. Parasitenkd 50:345–347Google Scholar
  4. Hadani A, Pipano E, Dinür Y (1969) Studies on the transmission ofTheileria annulata by ticks. J Protozool 16 Suppl 37:9–12Google Scholar
  5. Koch R (1906) Beiträge zur Entwicklungsgeschichte der Piroplasmen. Z Hyg Infektionskr 54:1–9Google Scholar
  6. Latt SA, Stetten G (1976) Spectral studies on 33258 Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of desoxyribonucleic acid synthesis. J Histochem Cytochem 24(1):24–33Google Scholar
  7. Martin HM, Barnett SF, Vidler BO (1964) Cyclic development and longevity ofTheileria parva in the tickRhipicephalus appendiculatus. Exp Parasitol 15:527–555Google Scholar
  8. Mehlhorn H, Schein E (1976) Elektronenmikroskopische Untersuchungen an Entwicklungsstadien vonTheileria parva (Theiler, 1904) im Darm der ÜberträgerzeckeHyalomma anatolicum excavatum (Koch, 1844). Z Tropenmed Parasitol 27:182–191Google Scholar
  9. Mehlhorn H, Schein E (1984) The piroplasms: life cycle and sexual stages. Adv Parasitol 23:38–103Google Scholar
  10. Mehlhorn H, Weber G, Schein E, Büscher G (1975) Elektronenmikroskopische Untersuchungen an Entwicklungsstadien vonTheileria annulata (Dschunkowsky, Luhs, 1904) im Darm und in der Hämolymphe vonHyalomma anatolicum excavatum (Koch, 1844). Z Parasitenkd 48:137–150Google Scholar
  11. Mehlhorn H, Schein E, Voigt WP (1980) Light and electron microscopic study on developmental stages ofBabesia canis within the gut of the tickDermacentor reticulatus. J Parasitol 66(2):220–228Google Scholar
  12. Nuttall GMF, Hindle E (1913) Conditions influencing the transmission of East Coast fever. Parasitology 6:321–332Google Scholar
  13. Riek RF (1964) The life cycle ofBabesia bigemina in the tick vectorBoophilus microplus. Aust J Agric Res 15:802–821Google Scholar
  14. Rudzinska MA, Spielman A, Riek RF, Lewengrub SJ, Piesman J (1979) Intraerythrocytic ‘gametocytes’ ofBabesia microti and their maturation in ticks. Can J Zool 57:424–434Google Scholar
  15. Rudzinska MA, Spielman A, Lewengrub SJ, Trager W, Piesman J (1983) Sexuality in piroplasms as revealed by electron microscopy inBabesia microti. Proc Natl Acad Sci USA 80:2966–2970Google Scholar
  16. Schein E (1975) On the life cycle ofTheileria annulata (Dschunkowsky and Luhs, 1904) in the midgut and hemolymph ofHyalomma anatolicum excavatum (Koch, 1844). Z Parasitenkd 47:165–167Google Scholar
  17. Schein E, Büscher G, Friedhoff KT (1975) Lichtmikroskopische Untersuchungen über die Entwicklung vonTheileria annulata (Dschunkowsky and Luhs, 1904) inHyalomma anatolicum excavatum Koch (1844): 1. Die Entwicklung im Darm vollgesogener Nymphen. Z Parasitenkd 48:123–136Google Scholar
  18. Simpson CF, Kirkham WW, Klin JM (1967) Comparative morphologic features ofBabesia caballi andB. equi. Am J Vet Res 28:1693–1697Google Scholar
  19. Weber G, Friedhoff KT (1977) Preliminary observations on the ultrastructure of supposed sexual stages ofBabesia bigemina (Piroplasmea). Z Parasitenkd 53:83–92Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Ute Mackenstedt
    • 1
  • Martina Gauer
    • 1
  • Heinz Mehlhorn
    • 1
  • Eberhard Schein
    • 2
  • Susann Hauschild
    • 1
  1. 1.Lehrstuhl für Spezielle Zoologie und ParasitologieRuhr-Universität BochumBochumGermany
  2. 2.Institut für Parasitologie und TropenveterinärmedizinFreie Universität BerlinBerlin 37

Personalised recommendations