Molecular and Cellular Biochemistry

, Volume 121, Issue 1, pp 93–98

Fractionation of erytroblasts with affinitymediated modifications of their electrical properties using counter-current distribution

  • Jesús Mendieta
  • Göte Johansson
Article
  • 12 Downloads

Abstract

We have previously reported the possibility of modifying the electrical properties of cells by means of their interaction with a specific ligand carrying a polyelectrolyte (Anal Biochem 200: 280–285). This selective modification of receptorcontaining cells changed their partition in a charge-sensitive aqueous two-phase system. We here present the fractionation of electrically modified erythroblasts by the use of an automatic multiple-partition procedure, counter-current distribution. The cells were fractionated according to the degree of differentiation of erythroblasts as evaluated from the hemoglobin content as well as the relative activities of the two enzymes, 3-phosphoglycerate kinase and bisphospho-glycerate mutase.

Key words

erythroid differentiation cell fractionation affinity modification two-phase system counter-current distribution 

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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Jesús Mendieta
    • 1
  • Göte Johansson
    • 1
  1. 1.Department of Biochemistry, Chemical CenterUniversity of LundLundSweden
  2. 2.C.E.C. Joint Research CentreGeel EstablishmentGeelBelgium

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