Molecular and Cellular Biochemistry

, Volume 140, Issue 1, pp 1–22 | Cite as

Physiological concentrations of purines and pyrimidines

  • Thomas W. Traut


The concentrations of bases, nucleosides, and nucleosides mono-, di- and tri-phosphate are compared for about 600 published values. The data are predominantly from mammalian cells and fluids. For the most important ribonucleotides average concentrations ±SD (μM) are: ATP, 3,152±1,698; GTP, 468±224; UTP, 567±460 and CTP, 278±242. For deoxynucleosidestriphosphate (dNTP), the concentrations in dividing cells are: dATP, 24±22; dGTP, 5.2±4.5; dCTP, 29±19 and dTTP 37±30. By comparison, dUTP is usually about 0.2 μM. For, the 4 dNTPs, tumor cells have concentrations of 6–11 fold over normal cells, and for the 4 NTPs, tumor cells also have concentrations 1.2–5 fold over the normal cells. By comparison, the concentrations of NTPs are significantly lower in various types of blood cells. The average concentration of bases and nucleosides in plasma and other extracellular fluids is generally in the range of 0.4–6 μM; these values are usually lower than corresponding intracellular concentrations. For phosphate compounds, average cellular concentrations are: Pi, 4400; ribose-1-P, 55; ribose-5-P, 70 and P-ribose-PP, 9.0. The metal ion magnesium, important for coordinating phosphates in nucleotides, has values (mM) of: free Mg2+, 1.1; complexed-Mg, 8.0. Consideration of experiments on the intracellular compartmentation of nucleotides shows support for this process between the cytoplasm and mitochondria, but not between the cytoplasm and the nucleus.

Key words

purines pyrimidines nucleosides nucleotides 


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  1. 1.
    Traut TW: Enzymes of nucleotide metabolism: The significance of subunit size and polymemr size for biological function and regulatory properties. CRC Crit Rev Biochem 23: 121–169, 1988Google Scholar
  2. 2.
    Traut TW: Uridine-5′-phosphate Synthase: Evidence for substrate cycling involving this bifunctional protein. Arch Biochem Biophys 268: 108–115, 1989Google Scholar
  3. 3.
    El Khouni MH, Naguib FNM, Park KS, Cha S, Darnowski JW, Soong S-J: Circadian rhythm of hepatic uridine phosphorylase activity and plasma concentrations of uridine in mice. Biochem Pharmacol 40: 2479–2485, 1990Google Scholar
  4. 4.
    Caradonna SJ, Adamkiewicz DM: Purification and properties of the deoxyguanosine triphosphate nucleotidohydrolase, enzyme derived from HeLa S3 cells. J Biol Chem 259: 5459–5464, 1984Google Scholar
  5. 5.
    Hochstadt J: The role of the membrane in the utilization of nucleic acid precursors. CRC Crit Rev Biochem 9: 259–310, 1974Google Scholar
  6. 6.
    Plagemann PGW, Wohlhueter RM, Erbe J Facilitated transport of inosine and uridine in cultured mammalian cells is independent of nucleoside phosphorylase. Biochim Biophys Acta 640: 448–462, 1981Google Scholar
  7. 7.
    Fuller S, Hutton JJ Meier J, Coleman MS: Deoxynucleotide-interconverting enzymes and the quantification of deoxynucleoside triphosphates in mammalian cells. Biochem J 206: 131–138, 1982Google Scholar
  8. 8.
    Kemp AJ, Lyons SD, Christopherson RI Effects of acivicin and dichloroallyl Lawsone upon pyrimidine biosynthesis in mouse L1210 leukemia cells. J Biol Chem 261: 14891–14895, 1986Google Scholar
  9. 9.
    Moyer JD, Henderson JF: Compartmentation of intracellular nucleotides in mammalian cells. CRC Crit Rev Biochem 19: 45–61, 1985Google Scholar
  10. 10.
    Siebert G: The limited contribution of the nuclear envelope to metabolic compartmentation. Biochem Soc Trans 6: 5–9, 1978Google Scholar
  11. 11.
    Kuebbing D, Werner R: A model for compartmentation ofde novo and salvage thymidine nucleotide pools in mammalian cells. Proc Natl Acad Sci USA 72: 3333–3336, 1975Google Scholar
  12. 12.
    Nicander B, Reichard P: Dynamics of pyrimidine deoxynucleoside triphosphate pools in relationship to DNA synthesis in 3T6 mouse fibroblasts. Proc Natl Acad Sci USA 80: 1347–1351, 1983Google Scholar
  13. 13.
    Plagemann PGW: Nucleotide pools of Novikoff hepatoma cells growing in suspension culture. II. Independent nucleotide pools for nucleic acid synthesis. J Cell Physiol 77: 241–258, 1971Google Scholar
  14. 14.
    Pels Rijken WR, Overdijk B, van den Eijnden DH, Ferwerda W: Pyrimidine nucleotide metabolism in rat hepatocytes: evidence for compartmentation of nucleotide pools. Biochem J 293: 207–213, 1993Google Scholar
  15. 15.
    Cheng N, Payne RC, Traut TW: Regulation of uridine kinase. Evidence for a regulatory site. J Biol Chem 261: 13006–13012 1986Google Scholar
  16. 16.
    Stärk D, Hannover R, Siebert G: Nucleotides and coenzymes in nuclei isolated from rat liver. Hoppe-Seyler's Z Physiol Chem 359: 1203–1208, 1978Google Scholar
  17. 17.
    Soboll S, Scholz R, Heldt HW: Subcellular metabolite concentrations. Dependence of mitochondrial and cytosolic ATP systems on the metabolic state of the perfused rat liver. Eur J Biochem 87 377–390, 1978Google Scholar
  18. 18.
    Akerboom TPM, Bookelman H, Zuurendonk PF, van der Meer R, Tager JM: Intramitochondrial and extramitochondrial concentrations of machine nucleotides and inorganic phosphate in isolated hepatocytes from fasted rats. Eur J Biochem 84: 413–420, 1978Google Scholar
  19. 19.
    Lavoinne A: Repartition of ATP, ADP and PO4 in isolated hepatocytes from fed and fasted rats. Biochimie 65: 71–75, 1983Google Scholar
  20. 20.
    Ropp PA, Traut TW: Allosteric regulation of purine nucleoside phosphorylase. Arch Biochem Biophys 288: 614–620, 1991Google Scholar
  21. 21.
    Downs SM, Coleman DL, Ward-Bailey PF, Eppig JJ: Hypoxanthine is the principal inhibitor of murine oocyte maturation in a low molecular weight fraction of porcine follicular fluid. Proc Natl Acad Sci USA 82: 454–458, 1985Google Scholar
  22. 22.
    Dudman NPB, Deveski WB, Tattersall MHN: Radioimmunoassays of plasma thymidine, uridine, deoxyuridine, and cytidine/deoxycytidine. Anal Biochem 115: 428–437, 1981Google Scholar
  23. 23.
    Werner A, Siems W, Gerber G: Purine and pyridine nucleotides in rabbit red blood cells of different maturity. Cell Biochem Funct 6: 251–256, 1988Google Scholar
  24. 24.
    Werner A, Siems W, Schmidt H, Rapoport I, Gerber G: Determination of nucleotides, nucleoside, and nucleobases in cells of different complexity by reverse-phase and ion-pair high-performance liquid chromatography. J Chromatog 421: 257–265, 1987Google Scholar
  25. 25.
    Pillwein K, Jayaram HN, Weber G: Effect of ischemia on nucleosides and bases in rat liver and hepatoma 3924A. Cancer Res 47: 3092–3096 1987Google Scholar
  26. 26.
    Arnold ST, Cysyk RL: Adenosine export from the liver: oxygen dependency. Am J Physiol 251: G34-G39, 1986Google Scholar
  27. 27.
    Rustum YM: High-performance liquid chromatography: I. Quantitative separation of purine and pyrimidine nucleosides and bases. Anal Biochem 90: 289–299, 1978Google Scholar
  28. 28.
    Fredholm BB: Analysis of purines. Life Sci 41: 837–840, 1987Google Scholar
  29. 29.
    Maguire MH, Westermeyer FA: Measurement of adenosine, inosine and hypoxanthine in human term placenta by reversed-phase high-performance liquid chromatography. J Chromatog 380: 55–66, 1986Google Scholar
  30. 30.
    Olivares J, Rossi A: Synthesis of pyrimidine nucleotides in rat myocardium: potential role of blood cytidine. J Mol Cell Cardiol 20: 313–322, 1988Google Scholar
  31. 31.
    Olivares J, Verdys M: Isocratic high-performance liquid chromatographic method for studying the metabolism of blood plasma pyrimidine nucleosides and bases: concentrations and radioactivity measurements. J Chromatog 434: 111–121, 1988Google Scholar
  32. 32.
    Moyer JD, Oliver JT, Handschumacher RE: Salvage of, circulating pyrimidine nucleosides in the rat. Cancer Res 41: 3010–3017, 1981Google Scholar
  33. 33.
    Staak S, Popov N, Matthies H: Effects of intraperitoneally-applied D-galactosamine on uridine and cytidine plasma content and brain activity of uridine kinase in the rat. Biomed Biochim Acta 48: 325–331, 1989Google Scholar
  34. 34.
    Gerrits GPJM, Monnens LAH, DeAbreu RA, Schröder CH, Trijbels JMF, Gabreëls FJM: Disturbances of cerebral purine and pyrimidine metabolism in young children with chronic renal failure. Nephron 58: 310–314, 1991Google Scholar
  35. 35.
    Berlinger WG, Stene RA, Spector R, Al-Jurf AS: Plasma and cerebrospinal fluid nucleosides and oxypurines in acute liver failure. J Lab Clin Med 110: 137–141, 1987Google Scholar
  36. 36.
    Steyn LM, Harley EH: Intracellular activity of HPRTCape Town: Purine uptake and growth of cultured cells in selective media. J Inher Metab Dis 8: 198–203, 1985Google Scholar
  37. 37.
    Hartwick RA, Krstulovic AM, Brown PR: Identification and quantitation of nucleosides, bases and other UV-absorbing compounds in serum, using reversed-phase high-performance chromatography. J Chromatog 186: 659–676, 1979Google Scholar
  38. 38.
    Akaoka I, Nishizawa T, Nishida Y: Determination of hypoxanthine and xanthine in plasma separated by thin-layer chromatography. Biochem Med 14: 285–289, 1975Google Scholar
  39. 39.
    McBurney A, Gibson T: Reverse phase partition HPLC for determination of plasma purines and pyrimidines in subjects with gout and renal failure. Clin Chim Acta 102: 19–28, 1980Google Scholar
  40. 40.
    Wung WE, Howell SB: Simultaneous liquid chromatography of 5-fluorouracil, uridine, hypoxanthine, xanthine, uric acid, allopurinol, and oxipurinol in plasma. Clin Chem 26: 1704–1708, 1980Google Scholar
  41. 41.
    Stene R, Spector R: Effect of a 400-kilocalorie carbohydrate diet on human plasma uridine and hypoxanthine concentrations. Biochem Med Metabol Biol 38: 44–46, 1987Google Scholar
  42. 42.
    Fürst W, Hallström S: Simultaneous determination of myocardial nucleotides, nucleosides, purine bases and creatine phosphate by ion-pair high-performance liquid chromatography. J Chromatog 578: 39–44, 1992Google Scholar
  43. 43.
    Mueller RA, Rosner MJ, Ghia JN, Powers SK, Kafer ER, Hunt RD: Alterations in cerebrospinal fluid uridine, hypoxanthine, and xanthine in head-injured patients. Cell Molec Neurobiol 8 235–243, 1988Google Scholar
  44. 44.
    Kish J, Fox IH, Kapur BM, Lloyd K, Hornykiewicz O: Brain benzodiazepine receptor binding and purine concentration in Lesh-Nyhan syndrome. Brain Res 336: 117–123, 1985Google Scholar
  45. 45.
    Harkness RA, Lund RJ: Cerebrospinal fluid concentrations of hypoxanthine, xanthine, uridine and inosine: high concentrations of the ATP metabolite, hypoxanthine, after hypoxia. J Clin Pathol 36: 1–8, 1983Google Scholar
  46. 46.
    Smith LH Jr, Huguley CM, Bain JA: In: J.B. Stanbury, J.B. Wyngaarden DS and D.S. Frederickson (eds). The Metabolic Basis of Inhorited Disease. McGraw-Hill, New York, 1972, pp 1003–1029Google Scholar
  47. 47.
    Hitchings GH: Indications for control mechanism in purine and pyrimidine biosynthesis as revealed by studies with inhibitors. Adv Enz Regul 12: 121–129, 1974Google Scholar
  48. 48.
    Pilz RB, Willis RC, Boss GR: The influence of ribose-5-phosphate availability on purine synthesis of cultured human lymphoblasts and mitogenstimulated lymphocytes. J Biol Chem 259: 2927–2935, 1984Google Scholar
  49. 49.
    Cortes P, Dumler F, Paielli DL, Levin NW: Glomerular uracil nucleotide synthesis: effects of diabetes and protein intake. Am J Physiol 255: F647-F655, 1988Google Scholar
  50. 50.
    Simmonds HA, Reiter S, Davies PM, Cameron JS: Orotidine accumulation in human erythrocytes during allopurinol therapy: association with high urinary oxypurinol-7-riboside concentrations in renal failure and in the Lesh-Nyhan syndrome. Clin Sci 80: 191–197, 1991Google Scholar
  51. 51.
    Schaer J-C, Maurer U, Schindler R: Determination of thymidine in serum used for cell culture media. Exp Cell Biol 46: 1–10, 1978Google Scholar
  52. 52.
    Hughes WL, Christine M, Stoller BD: A radioimmunoassay for measuring serum thymidine. Anal Biochem 55: 468–478, 1973Google Scholar
  53. 53.
    Bodycote J, Wolff S: Metabolic breakdown of3H-thymidine and the inability to measure human lymphocyte proliferation of incorporation of radioactivity. Proc Natl Acad Sci USA: 4749–4753, 1986Google Scholar
  54. 54.
    Van Groeningen CJ, Peters GJ, Nadal JC, Laurensse E, Pinede HM: Clinical and pharmacologic study of orally administered uridine. J Natl Canc Inst 83: 437–441, 1991Google Scholar
  55. 55.
    Parry TE, Blackmore JA: Serum ‘uracil + uridine’ levels before and after vitamine B12 therapy in pernicious anemia. J Clin Pathol 27: 789–793, 1974Google Scholar
  56. 56.
    Darnowski J, Handschumacher RE: Tissue uridine pools: evidencein vivo of a concentrative mechanism for uridine uptake. Cancer Res 46: 3490–3494, 1986Google Scholar
  57. 57.
    Chan TCK, Markman M, Cleary S, Howell SB: Plasma uridine changes in cancer patients treated with the combination of dipyridamole and N-phosphonacetyl-L-aspartate. Cancer Res 46: 3168–3172, 1986Google Scholar
  58. 58.
    Harkness RA, Simmonds RJ, Gough P, Priscott PK, Squire JA: Purine base and nucleoside, cytidine and uridine concentrations in foetal calf and other sera. Biochem Soc Trans 8: 139, 1980Google Scholar
  59. 59.
    Bismuth G, Thuillier L, Perignon J-L, Cartier PH: Uridine as the only alternative to pyrimidinede novo synthesis in rat T lymphocytes. FEBS Lett 148: 135–139, 1982Google Scholar
  60. 60.
    Karle JM, Anderson LW, Erlichman C, Cysyk RL: Serum uridine levels in patients receiving N-(phosphonacetyl)-L-aspartate. Cancer Res 40: 2938–2940, 1980Google Scholar
  61. 61.
    Karle JM, Anderson LW, Dietrick DD, Cysyk RL: Determination of serum and plasma uridine levels in mice, rats and humans by high preisure liquid chromatography. Anal Biochem 109: 41–46, 1980Google Scholar
  62. 62.
    Jackson RC: The regulation of thymidylate biosynthesis in Novikolf hepatoma cells and the effects of amethopterin, 5-fluorouridine, and 3-deazauridine. J Biol Chem 253: 7440–7446, 1978Google Scholar
  63. 63.
    Jackson RC, Lui MS, Boritzki TJ, Morris HP, Weber G: Purine and pyrimidine nucleotide patterns of normal, differentiating, and regenerating liver and of hepatomas in rats. Canc Res 40: 1286–1291, 1980Google Scholar
  64. 64.
    Hauschka PV: Analysis of nucleotide pools in animal cells. Meth Cell Biol 7: 361–462, 1973Google Scholar
  65. 65.
    North TW, Bestwick RK, Mathews CK: Detection of activities that interfere with the enzymatic assay of deoxyribonucleoside-5-triphosphates. J Biol Chem 255: 6640–6645, 1980Google Scholar
  66. 66.
    Skoog L, Bursell G: Nuclear and cytoplasmic pools of deoxyribonucleoside triphosphates in Chinese hamster ovary cells. J Biol Chem 249: 6434–6438, 1974Google Scholar
  67. 67.
    Lui MS, Faderman MA, Liepnieks JJ, Natsumeda Y, Olah E, Jayaram HN, Weber G: Modulation of IMP dehydrogenase activity and guanylate metabolism by tiazofurin (2-β-D-ribofuranosylthiazole-4-carboxamide). J Biol Chem 259: 5078–5082, 1984Google Scholar
  68. 68.
    Tyrsted G: The pool size of deoxyguanosine 5′-triphosphate and deoxycytidine 5′-triphosphate in phytohemagglutinin-stimulated and non-stimulated human lymphocytes. Exp Cell Res 91: 429–440, 1975Google Scholar
  69. 69.
    Maybaum J, Klein FK, Sadee WJ: Determination of pyrimidine ribotide and deoxyribotide pools in cultured cells and mouse liver by high-performance liquid chromatography. J Chromatog 188: 149–158, 1980Google Scholar
  70. 70.
    Goulian M, Bleile B, Tseng BY: Methotrexate-induced misincorporation of uracil into DNA. Proc Natl Acad Sci USA 77: 1956–1960, 1980Google Scholar
  71. 71.
    Olivera BM, Manlapaz-Ramos P, Warner HR, Duncan BK: DNA intermediates at theEscherichia coli replication fork. II. Studies usingdut andung mutantsin vitro. J Mol Biol 128: 265–275, 1979Google Scholar
  72. 72.
    Nilsson S, Reichard P, Skoog L: Deoxyuridine triphosphate pools after polyoma virus infection. J Biol Chem 255: 9552–9555, 1980Google Scholar
  73. 73.
    Just G, Holler E: Enhanced levels of cyclic AMP, adenosine (5′)tetraphospho(5′)adenosine and nucleoside 5′-triphosphates in mouse leukemia P388/D1 after treatment withcis-diaminedichloroplatinum(II). Biochem Pharmacol 42: 285–294, 1991Google Scholar
  74. 74.
    Marongiu ME, August EM, Prusoff WH: Effect of 3′-deoxythymidine-2′-ene (d4T) on nucleoside metabolism in H9 cells. Biochem Pharmacol 39: 1523–1528, 1990Google Scholar
  75. 75.
    Bishop C, Rankine DM, Talbott JH: The nucleotides in normal human blood. J Biol Chem 234: 1233–1237, 1959Google Scholar
  76. 76.
    Torrance JD, Whittaker D: Distribution of erythrocyte nucleotides in pyrimidine 5′-nucleotidase deficiency. Brit J Haematol 43: 423–434, 1979Google Scholar
  77. 77.
    Snyder FF, Cruikshank MK, Seegmiller JE: A comparison of purine metabolism and nucleotide pools in normal and hypoxanthine-guanine phosphoribosyltransferase-deficient neuroblastoma cells. Biochim Biophys Acta 543: 556–569, 1978Google Scholar
  78. 78.
    Faupel RP, Seitz HJ, Tarnowski W: The problem of tissue sampling from experimental animals with respect to freezing technique, anoxia, stress and narcosis. Arch Biochem Biophys 148: 509–522, 1972Google Scholar
  79. 79.
    Jackson RC, Boritzki TJ, Morris HP, Weber G: Purine and pyrimidine ribonucleotide contents of rat liver and hepatoma 3924A and the effect of ischaemia. Life Sci 19: 1531–1536, 1976Google Scholar
  80. 80.
    Woods RA, Henderson RM, Henderson JF: Consequences of inhibition of purine biosynthesisde novo by 6-mercaptopurine ribonucleoside in cultured lymphoma L5178 cells. Eur J Cancer 14: 765–770, 1978Google Scholar
  81. 81.
    Henderson JF, Zombor G: Effects of misonidazole on purine metabolism in Ehrlich ascites tumor cellsin vitro. Biochem Pharmacol 29: 2533–2536, 1980Google Scholar
  82. 82.
    Weber G, Lui MS, Jayaram HN, Pillwein K, Natsumeda Y, Faderman MA, Reardon MA: Regulation of purine and pyrimidine metabolism by insulin and by resistance to tiazofurin. Adv Enz Regul 23: 81–99, 1985Google Scholar
  83. 83.
    Keppler DO, Pausch J, Decker K: Selective uridine triphosphate deficiency induced by D-galactosamine in liver and reversed by pyrimidine nucleotide precursors. J Biol Chem 249: 211–216, 1974Google Scholar
  84. 84.
    Volkin E, Boling ME, Lee WH, Jones MH: The effect of chemical mutagens on purine and pyrimidine nucleotide biosynthesis. Biochim Biophys Acta 755: 217–224, 1983Google Scholar
  85. 85.
    Snyder FF, Seegmiller JE: The adenosine-like effect of exogenous cyclic AMP upon nucleotide and PP-ribose-P concentrations of cultured human lymphoblasts. FEBS Lett 66: 102–106, 1976Google Scholar
  86. 86.
    Sant ME, Poiner A, Harsanyi MC, Lyons SD, Christopherson RI: Chromatographic analysis of purine precursors in mouse L1210 leukemia. Anal Biochem 182: 121–128, 1989Google Scholar
  87. 87.
    De Korte D, Haverkort WA, Van Gennip AH, Roos D: Nucleotide profiles of normal human blood cells determined by high-performance liquid chromatography. Anal Biochem 147: 197–209, 1985Google Scholar
  88. 88.
    Pagani R, Tabuchi A, Carlucci F, Leoncini R, Consolmagno E, Molinelli M, Valerio P: Some aspects of purine nucleotide metabolism in human lymphocytes before and after infection with HIV-1 virus: nucleotide content. Adv Exp Med Biol 309B: 43–46, 1991Google Scholar
  89. 89.
    Hisanaga K, Onodera H, Kogure K: Changes in levels of purine and pyrimidine nucleotides during acute hypoxia and recovery in neonatal rat brain. J Neurochem 47: 1344–1350, 1986Google Scholar
  90. 90.
    Bates DJ, Perret D, Mowbray J: Systematic variations in the content of the purine nucleotides in the steady-state perfused rat heart. Biochem J 176: 485–493, 1978Google Scholar
  91. 91.
    Wright DG: A role for guanine ribonucleotides in regulation of myeloid cell maturation. Blood 69: 334–337, 1987Google Scholar
  92. 92.
    Henninger H, Holstege A, Herrmann B, Anukarahanonta T, Keppler DOR: Depletion of cytidine triphosphate as a consequence of cellslar uridine triphosphate deficiency. FEBS Lett 103: 165–167, 1979Google Scholar
  93. 93.
    Bowen JW, Levinson C: Phosphate concentration and transport in Ehrlich ascites tumor cells: Effect of sodium. J Cell Physiol 110: 149–154, 1982Google Scholar
  94. 94.
    Schwartz PM, Moir RD, Hyde CM, Turek PJ, Handschumacher RE: Role of uridine phosphorylase in the anabolism of 5-fluorouracil. Biochem Pharmacol 34: 3585–3589, 1985Google Scholar
  95. 95.
    Cory JG, Breland JC, Carter GL: Effects of 5-fluorouracil on RNA metabolism in Novikoff hepatoma cells. Cancer Res 39: 4905–4913, 1979Google Scholar
  96. 96.
    Peters GJ, Veerkamp JH: Concentration, synthesis and utilization of phosphoribosylpyrophosphate in lymphocytes of five mammalian species. Int J Biochem 10: 885–888, 1980Google Scholar
  97. 97.
    Hisata T: An accurate method for estimating 5-phosphoribosyl 1-pyrophosphate in animal tissues with the use of acid extraction. Anal Biochem 68: 448–457, 1975Google Scholar
  98. 98.
    May SR, Krooth RS: Determination of the intracellular concentration of 5′-phosphorribosyl-pyrophosphate in cultured mammalian fibroblasts. Anal Biochem 75: 389–401, 1976Google Scholar
  99. 99.
    Webb JL: Enzymes and Metabolic Inhibitors. Academic Press, New York, 1963Google Scholar
  100. 100.
    Gupta RK, Yushok WD: Noninvasive31P NMR probes of free Mg2+ MgATP, and MgADP in intact Ehrlich ascites tumor cells. Proc Natl Acad Sci USA 77: 2487–2491, 1980Google Scholar
  101. 101.
    Snyder FF, Henderson JF, Kim SC, Paterson ARP, Brox LW: Purine nucleotide metabolism and nucleotide pool sizes in synchronized lymphoma L5178Y cells. Cancer Res 33: 2425–2430, 1973Google Scholar
  102. 102.
    Cadman E, Benz C: Uridine and cytidine metabolism following inhibition ofde novo pyrimidine synthesis by pyrazofurin. Biochim Biophys Acta 609: 372–382, 1980Google Scholar
  103. 103.
    Veech RL, Lawson JWR, Cornell NW, Krebs HA: Cytosolic phosphorylation potential. J Biol Chem 254: 6538–6547, 1979Google Scholar
  104. 104.
    Goldenberg CJ, Stein WD: Increase in the affinity of the uridine phosphorylation system for ATP after serum or insulin activation of 3T3 fibroblasts. J Supramol Struct 9: 489–496, 1978Google Scholar
  105. 105.
    Bolla RI, Miller JK: Endogenous nucleotide pools and protein incorporation into liver nuclei from young and old rats. Mech Ageing Devel 12: 107–118, 1980Google Scholar
  106. 106.
    Scavennec J, Maraninchi D, Gastaut J-A, Carcassonne Y, Cailla HL: Purine and pyrimidine ribonucleoside monophosphate patterns of peripheral blood and bone marrow cells in human acute leukemias. Cancer Res 42: 1326–1330, 1982Google Scholar
  107. 107.
    Schlomai J, Kornberg AJ: Deoxyuridine triphosphatase ofEscherichia coli. J Biol Chem 253: 3305–3312, 1978Google Scholar

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Thomas W. Traut
    • 1
  1. 1.Department of Biochemistry and BiophysicsUniversity of North Carolina School of MedicineChapel HillUSA

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