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Maintenance of in vitro cultures ofBabesia divergens andBabesia major at low temperatures

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Cultures of erythrocytes parasitized byBabesia divergens andBabesia major were stored in medium cooled to 4°C for up to 8 weeks. There was a marked decrease in parasitaemia and an increase in the number of free extra-erythrocytic, unagglutinated merozoites, during the cooled period. Cultures stored in this way and returned to 38°C resumed growth, with or without sub-culture. At the low temperature, only one sub-culture is required per week.

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Correspondence to J. Konrad.

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Konrad, J., Canning, E.U., Phipps, L.P. et al. Maintenance of in vitro cultures ofBabesia divergens andBabesia major at low temperatures. Z. Parasitenkd. 71, 313–316 (1985). https://doi.org/10.1007/BF00928333

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