Parasitology Research

, Volume 76, Issue 4, pp 367–371 | Cite as

Comparison of isoenzyme profiles ofToxoplasma gondii tachyzoites produced under different culture conditions

  • M. L. Dardé
  • B. Bouteille
  • M. Pestre-Alexandre
Original Investigations


The isoenzyme profiles for ten enzymes of tachyzoites either grown in vivo in TG 180 mouse sarcoma cells or cultivated in vitro in human fibroblast cell culture were compared in eight cloned strains by means of isoelectric focusing in polyacrylamide gels. Fibroblast-cultivated tachyzoites of all strains tested showed an extra band in the LDH isoenzyme pattern that was not seen in tachyzoites from TG 180 mouse sarcoma cells. The isoenzyme patterns of four enzymes [aspartate aminotransferase (ASAT), glutathione reductase (GSR), glucose phosphate isomerase (GPI) and amylase] used to differentiate the strains did not change under the two different culture conditions. Nevertheless, the shift observed in the LDH isoenzyme pattern underlines the fact that isoenzyme analysis ofToxoplasma strains could be carried out with tachyzoites produced under the same culture conditions.


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  1. Bogliolo AR, Godfrey DG (1987) Isoenyzme changes during the life cycle ofTrypanosoma cruzi. Trans R Soc Trop Med Hyg 81:222–229PubMedGoogle Scholar
  2. Dardé ML, Bouteille B, Pestre-Alexandre M (1988a) Isoenzymic characterization of seven strains ofToxoplasma gondii by isoelectrofocusing in polyacrylamide gels. Am J Trop Med Hyg 39:551–558PubMedGoogle Scholar
  3. Dardé ML, Bouteille B, Pestre-Alexandre M (1988b) Analyse isoenzymatique de souches clonées deToxoplasma gondii. Bull Soc Fr Parasitol 6:41–46Google Scholar
  4. Desmonts G, Remington JS (1980) Direct agglutination test for diagnosis ofToxoplasma infection: method for increasing sensitivity and specificity. J Clin Microbiol 11:562–568PubMedGoogle Scholar
  5. Gardener PJ, Howells RE (1972) Isoenzyme variation in leishmanial parasites. J Protozool 19 [Suppl]:47–48Google Scholar
  6. Gillard BK (1979) Quantitative gel electrophoretic determination of serum amylase enzyme distributions. Clin Chem 25:1919–1923PubMedGoogle Scholar
  7. Harris H, Hopkinson DA (1976) Handbook of enzyme electrophoresis in human genetics. North Holland, AmsterdamGoogle Scholar
  8. Jacobs L, Remington JS, Melton ML (1969) A survey of meat samples from swine, cattle and sheep for the presence of encystedToxoplasma. J Parasitol 46:23–28Google Scholar
  9. Kreutzer RD, Sousa OE (1981) Biochemical characterization ofTrypanosoma spp. by isoenzyme electrophoresis. Am J Trop Med Hyg 30:308–317PubMedGoogle Scholar
  10. Martrou P, Pestre-Alexandre M, Loubet R, Nicolas JA, Malinvaud G (1965) La toxoplasmose congenitale (note concernant un cas mortel). Limousin Med 53:3–7Google Scholar
  11. Mirelman D (1987) Effect of culture conditions and bacterial associates on the zymodemes ofEntamoeba histolytica. Parasitol Today 3:37–43PubMedGoogle Scholar
  12. Mounier M (1982) Etude expérimentale de la toxoplasmose. A propos de quatre souches isolées chez l'homme et chez l'animal. Doctorat es Sciences Pharmaceutiques Thesis, LimogesGoogle Scholar
  13. Nerad TA, Daggett PM (1979) Starch gel electrophoresis: an effective method for separation of pathogenic and non pathogenicNaegleria strains. J Protozool 26:613–615PubMedGoogle Scholar
  14. Roques C, Bessières MH, Seguela JP (1986) Caractérisation immunochimique des protéines des exo-antigènes provenant de différentes souches deToxoplasma gondii. Bull Soc Fr Parasitol 4:79–84Google Scholar
  15. Rowe E, Gibson I, Cavill A (1971) The effects of growth conditions on the esterases ofParamecium aurelia. Biochem Genet 5:151–159Google Scholar
  16. Sabin AB (1941) Toxoplasmic encephalitis in children. J Am Med Assoc 116:801–807Google Scholar
  17. Suzuki Y, Conley FK, Remington JS (1989) Differences in virulence and development of encephalitis during chronic infection vary with the strain ofToxoplasma gondii. J Infect Dis 159:790–794PubMedGoogle Scholar
  18. Ware PL, Kasper LH (1987) Strain specific antigens ofToxoplasma gondii. Infect Immun 55:778–783PubMedGoogle Scholar
  19. Weinbreck P, Leymarie JL, Vidal J, Gobeaux RF, Dardé ML, Denis F, Leboutet MJ, Bordessoule D, Pestre-Alexandre M (1985) Toxoplasmose cérébrale et SIDA: intérêts de la biopsie neurochirurgicale précoce. Med Mal Inf [A] 5b:320Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • M. L. Dardé
    • 1
  • B. Bouteille
    • 1
  • M. Pestre-Alexandre
    • 1
  1. 1.Service de ParasitologieCHU DupuytrenLimoges CédexFrance

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