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Zeitschrift für Parasitenkunde

, Volume 60, Issue 2, pp 135–146 | Cite as

Modes d'entrée des trophozoïtes deToxoplasma gondii dans les macrophages péritonéaux de souris normales et dans les cellules Hela en culture. Une étude microcinématographique en contraste de phase

  • Bao Tho Nguyen
  • Simone Stadtsbaeder
Original Investigations

Résumé

Le mode d'entrée des trophozoïtes vivants deToxoplasma gondii (souche RH) dans les macrophages péritonéaux de souris normales et dans les cellules HeLa est étudié en microcinématographie en contraste de phase. Les résultats ont montré queToxoplasma peut entrer dans les macrophages par phagocytose et/ou par pénétration active. Seul le second processus a été observé avec les cellules HeLa normalement non phagocytaires. Au cours de ce processus, les parasites se déplacent activement vers les cellules-hôtes par flexion et les pénètrent toujours par leur extrémité la plus effilée. La pénétration active est un phénomène rapide (environ 20 secondes à37°C) et est accompagnée par une série de modifications morphologiques p.e., allongment de l'extrémité antérieure, contraction et gonflement du corps du parasite. Contrastant avec la phagocytose, les toxoplasmes qui ont pénétré activement dans la cellule ne sont pas immédiatement isolés du cytoplasme de l'hôte par une vacuole visible au microscope optique. La nature du processus de pénétration (pression et/ou perforation de la membrane plasmique) est discutée.

Modes of entry ofToxoplasma gondii trophozoites into normal mouse peritoneal macrophage and Hela cell monolayers. A phase-contrast microcinematographic study

Summary

The mode of entry of living trophozoites ofToxoplasma gondii (RH strain) into normal mouse peritoneal macrophage and HeLa cell monolayers was studied by phase-contrast microcinematography. The results have shown thatToxoplasma can enter into macrophages either by phagocytosis (Figs. 1 and 2) and/or by active penetration (Fig. 3). Only the latter process was observed with normally non-phagocytic HeLa cells (Fig. 4). During this process the parasites actively moved towards the host-cells by flexion and penetrated them always through their sharpest end. Active penetration was a rapid phenomenon (about 20 s at 37°C) and was accompanied by a series of morphological changes, i.e., elongation of the anterior end, contraction and swelling of the parasite body. Contrasting with phagocytosis, toxoplasmas which had penetrated into the cell were not immediately isolated from the host-cytoplasm by a microscopically discernable vacuole. The nature of the process of penetration (pressure and/or perforation of the plasma membrane) is discussed.

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Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Bao Tho Nguyen
    • 1
  • Simone Stadtsbaeder
    • 1
  1. 1.Service de Microbiologie, Cliniques Universitaires Saint-Luc, UCL 10/1752Université Catholique de LouvainBruxellesBelgique

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