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Zeitschrift für Parasitenkunde

, Volume 69, Issue 4, pp 415–424 | Cite as

Liposomal chemotherapy in visceral leishmaniasis: An ultrastructural study of an intracellular pathway

  • John S. Weldon
  • John F. Munnell
  • William L. Hanson
  • Carl R. Alving
Original Investigations

Abstract

The intracellular fate of liposomes administered intracardially was examined in the liver and spleen of hamsters experimentally infected withLeishmania donovani. Separate groups of animals were treated with liposomes containing either an antileishmanial agent, a colloidal gold marker, or saline. Ultrastructural examinations of lysosomal interactions with the parasitophorous vacuole and with phagocytized liposomes were made. Lysosomes readily fused with the parasitophorous vacuoles but appeared to have little effect on the parasite, possibly due to the production of enzyme inhibitors. Liposomes rapidly became localized in lysosomes subsequent to endocytosis by macrophages. Morphologic evidence suggested that secondary lysosomes containing liposomal residues then fused with the parasitophorous vacuole. Aspects of one possible pathway are discussed which may account for the greatly enhanced effectiveness of liposomal chemotherapy for experimental visceral leishmaniasis.

Keywords

Gold Enzyme Inhibitor Separate Group Visceral Leishmaniasis Leishmaniasis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • John S. Weldon
    • 1
  • John F. Munnell
    • 1
  • William L. Hanson
    • 2
  • Carl R. Alving
    • 3
  1. 1.Department of Anatomy and RadiologyUniversity of GeorgiaAthensUSA
  2. 2.Department of Parasitology; College of Veterinary MedicineUniversity of GeorgiaAthensUSA
  3. 3.Department of Membrane BiochemistryWalter Reed Army Institute of ResearchWashington, DCUSA

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