Zeitschrift für Parasitenkunde

, Volume 63, Issue 2, pp 113–128

Light and electron microscopic studies on the effect of praziquantel onSchistosoma mansoni, Dicrocoelium dendriticum, andFasciola hepatica (trematoda) in vitro

  • B. Becker
  • H. Mehlhorn
  • P. Andrews
  • H. Thomas
  • J. Eckert
Original Investigations

Abstract

The fine structure of the tegument of three trematode species,Schistosoma mansoni, Dicrocoelium dendriticum, andFasciola hepatica, was studied by means of light scanning (SEM) and transmission electron microscopy (TEM) after in vitro exposure to 0, 1,10, and 100 μg/ml of the anthelmintic praziquantel for 5, 15, 30, and 60 min. InS. mansoni andD. dendriticum the resulting vacuolization of the tegument was confined to numerous small areas scattered all over the surface of the parasites and this finally led to the disruption of the apical tegumental layer. No changes were found in the tegument ofF. hepatica after treatment with praziquantel.

Key words

Schistosoma mansoni Dicrocoelium dendriticum Fasciola hepatica Praziquantel Fine structure Tegument 

Abbreviations

B

Bubbles

BL

Basement layer

CM

Circular musculature

GA

Genital atrium

GC

Gynaecophoric canal

IB

Inclusion bodies

IM

Interstitial material

LM

Longitudinal musculature

MI

Mitochondria

OS

Oral sucker

R

Ridges

S

Spine

SP

Surface pits

T

Tubercle

TG

Tegument

V

Vacuoles

VS

Ventral sucker

SEM

Scanning electron microscopy

TEM

Transmission electron microscopy

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References

  1. Andrews P (1976) Pharmacokinetic studies with Droncit® in animals using a biological assay. Vet Med Rev 10: 154–165Google Scholar
  2. Andrews P, Thomas H (1979) The effect of praziquantel onHymenolepis diminuta in vitro. Tropenmed Parasitol 30:391–400PubMedGoogle Scholar
  3. Becker B, Mehlhorn H, Andrews P, Thomas H (1980) Scanning and transmission electron microscope studies on the efficacy of praziquantel inHymenolepis nana (Cestoda) in vitro. Z Parasitenkd 61:121–133CrossRefPubMedGoogle Scholar
  4. Bennett CE, Threadgold LT (1975)Fasciola hepatica: Development of tegument during migration in mouse. Exp Parasitol 38:38–55CrossRefPubMedGoogle Scholar
  5. Borgers M, De Nollin S, Verheyen A, Vanparijs O, Thienpont D (1975) Morphological changes in cysticerci ofTaenia taeniaeformis after mebendazole treatment J Parasitol 61:830–843CrossRefPubMedGoogle Scholar
  6. Erasmus DA (1967) The host-parasite interface ofCyathocotyle bushiensis Khan, 1962 (Trematoda: Strigeoidea). II. Electron microscope studies of the tegument. J Parasitol 53:703–714CrossRefPubMedGoogle Scholar
  7. Gönnert R (1955) Schistosomiasis-Studien. III. Über die Einwirkung von Miracil D aufSchistosoma mansoni im Mäuseversuch und die Verteilung des Pigmentes in der Wirtsleber. Z Tropenmed 6:257–279Google Scholar
  8. Gönnert R, Andrews P (1977) Praziquantel, a new broad-spectrum antischistosomal agent. Z Parasitenkd 52:129–150CrossRefPubMedGoogle Scholar
  9. Hockley DJ (1973) Ultrastructure of the tegument ofSchistosoma. Adv Parasitol 11:233–305CrossRefPubMedGoogle Scholar
  10. James C, Webbe G, Nelson GS (1977) The susceptibility to praziquantel ofSchistosoma haematobium in the baboon (Papio anubis) and ofS. japonicum in the vervet monkey (Cercopithecus aethiops). Z Parasitenkd 52:179–194CrossRefPubMedGoogle Scholar
  11. Leopold G, Ungethüm W, Groll E, Diekmann W, Wegner H, Wegner DHG (1978) Clinical pharmacology in normal volunteers of praziquantel, a new drug against schistosomes and cestodes. Eur J Clin Pharmacol 14:281–291CrossRefPubMedGoogle Scholar
  12. Morris GP, Threadgold LT (1968) Ultrastructure of the tegument of adultSchistosoma mansoni. J Parasitol 54:15–27CrossRefPubMedGoogle Scholar
  13. Patzschke K, Pütter J, Wegner LA, Horster FA, Diekmann HW (1979) Serum concentration and renal excretion in humans after oral administration of praziquantel — results of three determination methods. Eur J Drug Metab Pharmacokin 4:149–159CrossRefGoogle Scholar
  14. Smith JH, Reynolds ES, von Lichtenberg F (1969) The integument ofSchistosoma mansoni. Am J Trop Med Hyg 18:28–49PubMedGoogle Scholar
  15. Steiner K, Garbe A, Diekmann HW, Nowak H (1976) The fate of praziquantel in the organism. I. Pharmacokinetics in animals. Eur J Drug Metab Pharmacokin 2:85–95CrossRefGoogle Scholar
  16. Thièry JP (1967) Mise en évidence des polysaccharides sur coupes fines en microscopie électronique. J Microsc 6:987–1018Google Scholar
  17. Thomas H, Gönnert R (1977) The efficacy of praziquantel against cestodes in animals. Z Parasitenkd 52:117–127CrossRefPubMedGoogle Scholar
  18. Threadgold LT (1963) The tegument and associated structures ofFasciola hepatica. Q J Microsc Soc 104:505–512Google Scholar
  19. Verheyen A, Vanparijs O, Borgers M, Thienpont D (1978) Scanning electron microscopic observations ofCysticercus fasciolaris (=Taenia taeniaeformis) after treatment of mice with mebendazole. J Parasitol 64:411–425CrossRefPubMedGoogle Scholar
  20. Webbe G, James C (1977) A comparison of the susceptibility of praziquantel ofSchistosoma haematobium, S. japonicum, S. mansoni, S. intercalatum, andS. matthaei in hamsters. Z Parasitenkd 52:169–177CrossRefPubMedGoogle Scholar
  21. Wilson RA, Barnes PE (1977) The formation and turnover of the membranocalyx on the tegument ofSchistosoma mansoni. Parasitology 74:61–71CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • B. Becker
    • 1
  • H. Mehlhorn
    • 1
  • P. Andrews
    • 2
  • H. Thomas
    • 2
  • J. Eckert
    • 3
  1. 1.Institut für Zoologie II der Universität DüsseldorfDüsseldorf 1Germany
  2. 2.Bayer Pharma-ForschungszentrumInstitut für ChemotherapieWuppertal 1Germany
  3. 3.Institut für Parasitologie der Universität ZürichZürichSwitzerland

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