Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Curcumin inhibits nitrogen dioxide induced oxidation of hemoglobin


Curcumin protects hemoglobin from nitrogen dioxide induced oxidation. Curcumin was also found to scavenge nitrogen dioxide in a concentration dependent way. The study also explains the ability of curcumin to protect hemoglobin from nitrite induced oxidation, where nitrogen dioxide is a key intermediate.

This is a preview of subscription content, log in to check access.


  1. 1.

    Gaston B, Drazen JM, Loscalzo J, Stamler JS: Am J Respir Crit Care Med 149: 538–551, 1994

  2. 2.

    Mohensin V, Gee JBL: Acute effect of nitrogen dioxide exposure on functional activity of alpha-1-protease inhibitor on broncho alveolar lavages fluid of normal subjects. Am Rev Resp Dis 136: 646–650, 1987

  3. 3.

    Beckman JS, Beckman TW, Chen J, Marshall PA, Freeman BA: Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide. Proc Nat Acad Sci USA 87: 1620–1624, 1990

  4. 4.

    Radi R, Beckman JS, Bush KM, Freeman BA: Peroxynitrite-induced membrane lipid peroxidation: the cytotoxic potential of superoxide and nitric oxide. Arch Biochem Biophys 288: 481–487, 1991

  5. 5.

    Chiodi H, Cillier CR, Mohler JG:In vitro methemoglobin formation in human blood exposed to nitrogen dioxide. Environ Res 30: 9–15, 1983

  6. 6.

    Halliwell B, Hu ML, Louie S, Duvall TR, Tarkington BK, Motchnik P, Cross CE: Interaction of nitrogen dioxide with human plasma: antioxidant depletion and oxidative damage. FEBS Letts 313: 62–66, 1992

  7. 7.

    Srimal RC, Dhavan BN: Curcumin. Drugs Future 12: 331–333, 1987

  8. 8.

    Kunchandi E, Rao NMA: Oxygen radical scavenging activity of curcumin. Int J Pharmaceutics 58: 237–240, 1990

  9. 9.

    Tonnssen HH, Greenhill J: Studies on curcumin and curcuminoids XXII, curcumin as a reducing agent and radical scavenger. Int J Pharmaceutics 87: 79–87, 1992

  10. 10.

    Unnikrishnan MK, Rao MNA: Curcumin inhibits nitrite induced methemoglobin formation. FEBS Letts 301: 195–196, 1992

  11. 11.

    Sreejayan, Rao MNA: Curcumin inhibits iron dependent lipid peroxidation. Int J Pharmaceutics 100: 93–97, 1993

  12. 12.

    Doyle MP, Pickering RA, Dykstra RL, Nelson CL, Boyer RF: Involvement of peroxide and superoxide in the oxidation of hemoglobin by nitrite. Biochem Biophys Res Comm 105: 127–132, 1982

  13. 13.

    Winterbourn CC: Oxidative reactions of hemoglobin. Methods Enzymol 186: 267–272, 1990

  14. 14.

    Stecher PG: Merck Index (7th edn). Merck and Co, USA, 1960, p 727

  15. 15.

    Kosoka H, Tyuma I: Mechanism of autocatalytic oxidation of oxyhemoglobin by nitrite. Environ Health Perspect 73: 147–151, 1987

  16. 16.

    Kosoka H, Uozomi M: Inhibition by amines indicate involvement of nitrogen dioxide in autocatalytic oxidation of oxyhemoglobin by nitrite. Biochim Biophys Acta 871: 14–18, 1986

  17. 17.

    Ammon HP, Wahl MA: Pharmacology of curcumin. Planta Med 57: 1–7, 1991

Download references

Author information

Correspondence to M. N. A. Rao.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Unnikrishnan, M.K., Rao, M.N.A. Curcumin inhibits nitrogen dioxide induced oxidation of hemoglobin. Mol Cell Biochem 146, 35–37 (1995).

Download citation

Key words

  • curcumin
  • hemoglobin
  • nitrogen dioxide
  • free radical scavenger