Molecular and Cellular Biochemistry

, Volume 125, Issue 1, pp 11–18 | Cite as

Acute treatment with tumour necrosis factor-α induces changes in protein metabolism in rat skeletal muscle

  • Cèlia García-Martínez
  • Francisco J. López-Soriano
  • Josep M. Argilés
Article

Abstract

Acute treatment of rats with recombinant tumour necrosis factor (TNF-α) caused an enhanced proteolytic rate —measured as tyrosine released in the presence of cycloheximide — insoleus muscle (34%). The cytokine treatment also decreased the rate of protein synthesis in this muscle (22%) while it had no effect upon the same parameter inextensor digitorum longus (EDL) (26%) muscle. In addition, treatment of rats with TNF-α increased amino acid uptake by transport system A in the incubated muscles both insoleus (45%) andEDL (99%) in the presence of insulin in the incubating medium. This effect was not associated with a direct action of TNF on muscle since the addition of different concentrations of the cytokine to the preparations did not alter the uptake of α-(methyl)-aminoisobutyric acid by the incubated muscles. It can be concluded that acute TNF-α treatment causes changes in protein metabolism in red-type muscles — suchsoleus — while little effects are seen in white-type muscles — such as EDL. The results presented may, to some extent, be related to the cachectic response associated with cancer and inflammation.

Key Words

tumor necrosis factor cytokines protein metabolism skeletal muscle 

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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Cèlia García-Martínez
    • 1
  • Francisco J. López-Soriano
    • 1
  • Josep M. Argilés
    • 1
  1. 1.Unitat de Bioquímica B, Departament de Bioquímica i Fisiologia, Facultat de BiologiaUniversitat de BarcelonaBarcelonaSpain

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