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Cytotoxicity ofEchinococcus granulosus cyst fluid in vitro

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Abstract

In a cytotoxicity assay, using rat spleen cells as target cells,Echinococcus granulosus cyst fluid from cattle exerted a marked degree of cytotoxicity in vitro. When trypan blue exclusion or [3H]thymidine incorporation by concanavalin A stimulated spleen cells was used as a measure of cell viability, dialyzed cyst fluid showed maximum cell destruction up to a 1:8 dilution. The effect was dose and time dependent, cells being affected by 24 h after exposure to cyst fluid. The components responsible for cytotoxicity of cyst fluid were heat stable and could be recovered using gel chromatography on Sephadex G 50 and G 15 as a single low molecular weight fraction. It is assumed that the toxic products released by the living parasite can readily penetrate through the cyst wall into the surrounding host tissue. The interference of such substances with immunocompetent cells might account for the long-term survival of the parasite in the intermediate host.

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References

  1. Adler WH, Takiguchi T, Marsh B, Smith RT (1970) Cellular recognition by mouse lymphocytes in vitro. I. Definition of a new technique and results of stimulation by phytohemagglutinin and specific antigens. J Exp Med 131:1049–1078

  2. Ali-Khan Z (1974) Host-parasite relationship in Echinococcosis. I. Parasite biomass and antibody response in three strains of inbred mice against graded doses ofEchinococcus multilocularis cysts. J Parasitol 60:231–235

  3. Ali-Khan Z (1978) Pathological changes in the lymphoreticular tissues of Swiss mice infected withEchinococcus granulosus cysts. Z Parasitenkd 58:47–54

  4. Araj GF, Matossian RM, Frayha GJ (1977a) The host response in secondary hydatidosis of mice. I. Circulating antibodies. Z Parasitenkd 52:23–30

  5. Araj GF, Matossian RM, Malakian AH (1977b) The host response in secondary hydatidosis of mice. II. Cell mediated immunity. Z Parasitenkd 52:31–38

  6. Baron RW, Tanner CE (1976) The effect of immunosuppression on secondaryEchinococcus multilocularis infections in mice. Int J Parasitol 6:37–42

  7. Cameron TWM, Webster GA (1969) The histogenesis of the hydatid cyst (Echinococcus spp.). Part I. Liver cysts in large mammals. Can J Zool 47:1405–1410

  8. Coltorti EA, Varela-Diaz VM (1975) Penetration of host IgG molecules into hydatid cysts. Z Parasitenkd 48:47–51

  9. Deschiens R, Poirier M (1947) L'intoxication expérimentale par le liquide hydatique. CR Soc Biol (Paris) 141:1161–1163

  10. Deschiens R, Poirier M (1948) L'intoxication chronique du cobaye par l'extrait trichloracétique de liquide hydatique. CR Soc Biol (Paris) 142:435–436

  11. Giusti L, Hug E (1923) Propriétés pharmacodynamiques du liquide hydatique. CR Soc Biol (Paris) 88:344–346

  12. Kagan IG (1976) Serodiagnosis of hydatid disease. In: Cohen S, Sadun E (eds) Immunology of parasitic infections, Blackwell Scientific Publications, Oxford, p 130

  13. Leid RW (1977) Immunity to the metacestode ofTaenia taeniaeformis in the laboratory rat. Am J Trop Med Hyg 26:54–60

  14. Lemaire G (1936) Effets physiologiques différents des albumines hydatiques et de la toxine isolée par ultrafiltration. CR Soc Biol (Paris) 121:349–350

  15. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

  16. Pauluzzi S (1969) Serologic response of mice and rats to secondary, experimental hydatid disease. Am J Trop Med Hyg 18:7–12

  17. Rau ME, Tanner CE (1973)Echinococcus multilocularis in the cotton rat. The effect of preexisting subcutaneous cysts on the development of a subsequent intraperitoneal inoculum of protoscolices. Can J Zool 51:55–59

  18. Rau ME, Tanner CE (1976a)Echinococcus multilocularis in the cotton rat: The in vitro protoscolicidal activity of peritoneal cells. Int J Parasitol 6:195–198

  19. Rau ME, Tanner CE (1976b)Echinococcus multilocularis in the cotton rat: Growth of intrathoracic metastases following surgical removal of subcutaneous cysts. Int J Parasitol 6:151–153

  20. Rausch R (1954) Studies on the helminth fauna of Alaska. XX. The histogenesis of the alveolar larva ofEchinococcus species. J Infect Dis 94:178–186

  21. Reisin IL, Rabito CA, Rotunno CA, Cereijido M (1977) The permeability of the membranes of experimental secondary cysts ofEchinococcus granulosus to [14C]mebendazole. Int J Parasitol 7:189–194

  22. Reuben JM, Tanner CE, Rau ME (1978) Immunoprophylaxis with BCG of experimentalEchinococcus multilocularis infections. Infect Immun 21:135–139

  23. Rickard MD (1974) Hypothesis for the long-term survival ofTaenia pisiformis cysticerci in rabbits. Z Parasitenkd 44:203–209

  24. Schantz PM (1977)Echinococcus granulosus: Acute systemic allergic reactions to hydatid cyst fluid in infected sheep. Exp Parasitol 43:268–285

  25. Svilenov D, Heymer B, Haferkamp O (1978) Immunomorphology and pathogenesis of echinococcosis. III. Microscopic immunofluorescence studies in experimental alveolar echinococcosis. Infection 6:12–15

  26. Tabatabai M, Ismaili MH, Nazarian I, Daneshbod K (1974) Pathophysiological changes resulting from intravenous injection of ovine hydatid cyst fluid to sheep. Br J Exp Pathol 55:33–37

  27. Varela-Diaz VM, Coltorti EA (1973) The presence of host immunoglobulins in hydatid cyst membranes. J Parasitol 59:484–488

  28. Varela-Diaz VM, Coltorti EA (1974) Recent studies on the penetration of macromolecules into hydatid cysts. Proceedings of 3rd International Conference of Parasitology. Munich, 1:574–575. Facta Pupl. Vienna

  29. Vogel H (1977) Über denEchinococcus multilocularis Süddeutschlands. II. Entwicklung der Larvenstadien und histopathologische Reaktionen in der Feldmaus,Microtus arvalis. Tropenmed Parasitol 28:409–427

  30. Warren KS (1978) In: Samter M (ed) Immunological diseases, Little Brown, Boston, p 722

  31. Williams JF (1979) Recent advances in the immunology of cestode infections. J Parasitol 65:337–349

  32. Yusuf JN, Frayha GJ, Malakian AH (1975)Echinococcus granulosus: Host lymphocyte transformation by parasite antigens. Exp Parasitol 38:39–37

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Dedicated to Prof. Dr. G. Piekarski on the occasion of his 70th birthday

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Annen, J.M., Köhler, P. & Eckert, J. Cytotoxicity ofEchinococcus granulosus cyst fluid in vitro. Z. Parasitenkd. 65, 79–88 (1981). https://doi.org/10.1007/BF00926556

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Key words

  • Echinococcus granulosus
  • Cyst fluid
  • Cytotoxicity
  • Spleen cells
  • Immune evasion