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Molecular and Cellular Biochemistry

, Volume 120, Issue 2, pp 119–126 | Cite as

Influence of liposome charge and composition on their interaction with human blood serum proteins

  • Trinidad Hernández-Caselles
  • José Villalaín
  • Juan C. Gómez-Fernández
Article

Abstract

Lipid composition and specially their electrostatic properties, were found to greatly influence the stability of liposomes in human blood serum. The amount and type of serum proteins bound to the liposomes were also clearly influenced by lipid composition and charge of liposomes. a good correlation was found between the amount of serum proteins adsorbed to a given type of liposome and its instability as measured by the release of an encapsulated fluorescent probe. Liposomes that bind the highest amount of protein were the least stable, except for the case of liposomes containing gangliosides, which were fairly stable even at a high amount of bound protein. Liposomes with neutral charge containing phosphatidylcholine were the most stable and bound the lowest amount of protein. Liposomes with positive charge behaved similarly to those with neutral charge. However, the stability of negatively charged liposomes was very dependent on their composition. Those liposomes containing only one class of negatively charged phospholipids bound a great amount of protein and were very unstable. However, those liposomes containing also phosphatidylcholine bound less protein and were more stable. The examination of the electrophoresis patterns of serum proteins bound to the different types of liposomes indicated the presence of specific proteins which correlated with liposome instability. (Mol Cell Biochem120: 119–126, 1993)

Key Words

liposome stability liposome-blood interaction 

Abbreviations

CF

6-carboxyfluorescein

Chol

Cholesterol

DCP

Dicetylphosphate

DPPC

Dipalmitoylphosphatidylcholine

GC

Galactocerebroside

GM1

Monosyaloganglioside

PC

Phosphatidylcholine

PG

Phosphatidylglycerol

PI

Phosphatidylinositol

PS

Phosphatidylserine

RES

Reticuloendothelial system

SA

Stearylamine

SM

Sphingomyelin

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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Trinidad Hernández-Caselles
    • 1
  • José Villalaín
    • 1
  • Juan C. Gómez-Fernández
    • 1
  1. 1.Departamento de Bioquímica y Biología Molecular A, Facultad de VeterinariaUniversidad de MurciaMurciaSpain

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