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PCR-based analysis of voltage-gated K+ channels in vascular smooth muscle

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Abstract

Irregularities in K+ currents form the basis of several cardiovascular dysfunctions, among which are arrhythmias and vasospasms. The developmental regulation of voltage-gated K+ channels, however, has been difficult to study. A novel approach was therefore employed to examine these channels in muscle tissue. Primers for a PCR-based analysis were designed using published nucleic acid sequences for voltage-gated K+ channels. Final selection of the primer pairs was based on the homology present in the S4 and H5 transmembrane domains. A specific band was amplified with these primers using RNA isolated from both rat A10 vascular smooth muscle cells and rat heart tissue.

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Zahradka, P., Harris, K.D., Triggs-Raine, B. et al. PCR-based analysis of voltage-gated K+ channels in vascular smooth muscle. Mol Cell Biochem 145, 39–44 (1995). https://doi.org/10.1007/BF00925711

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Keywords

  • Smooth Muscle
  • Muscle Cell
  • Nucleic Acid
  • Smooth Muscle Cell
  • Prime Pair