Cellular fibronectin and tenascin in an orbital nylon prosthesis removed because of infection caused byStaphylococcus aureus

  • Taru Päällysaho
  • Kaarina Tervo
  • Tero Kivelä
  • Ismo Virtanen
  • Ahti Tarkkanen
  • Timo Tervo
Clinical Investigations

Abstract

An orbital nylon prosthesis was removed because of an infection caused byStaphylococcus aureus that was resistant to antimicrobials. It was processed for histopathology and immunohistochemistry. Within 3 weeks the implant had an extensive ingrowth of fibrovascular tissue containing chronic inflammatory cells, foreign body giant cells, and myofibroblasts. By using the indirect immunofluorescent method, this tissue was found to react with monoclonal antibodies (Mabs) against extradomain A of cellular fibronectin (EDA-cFN) and tenascin (TN). The presence of EDA-cFN and TN within the implant are indicative of an active healing process, since both of these proteins, scarce in adult tissues, have been shown to be reexpressed during tissue regeneration. The findings suggest that fibronectin plays a definite role in bacterial adherence and foreign body infections.

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References

  1. 1.
    Albeda SM, Buck CA (1990) Integrins and other cell adhesion molecules. FASEB J 4:2868–2880Google Scholar
  2. 2.
    Aufderheide E, Ekblom P (1988) Tenascin during gut development: appearance in the mesenchyme, shift in molecular forms, and dependence on epithelial-mesenchymal interactions. J Cell Biol l07:2341–2349Google Scholar
  3. 3.
    Bourdon MA, Ruoslahti E (1989) Tenascin mediates cell attachment through an RGD-dependent receptor. J Cell Biol 108:1149–1155Google Scholar
  4. 4.
    Chhatwall GS, Preissner KT, Müller-Berghaus G, Blobel H (1987) Specific binding of the human S protein (vitronectin) to streptococci,Staphylococcus aureus, andEscherichia coli. Infect Immun 55:1878–1883Google Scholar
  5. 5.
    Chiquet-Ehrismann R, Mackie EJ, Pearson CA, Sakakura T (1986) Tenascin: an extracellular matrix protein involved in tissue interactions during fetal development and oncogenesis. Cell 47:131–139Google Scholar
  6. 6.
    Chuong C-M, Chen H-M (1991) Enhanced expression of neural cell adhesion molecules and Tnascin (cytotactin) during wound healing. Am J Pathol 138:427–440Google Scholar
  7. 7.
    Costeron JW, Irvin RT, Cheng KJ (1981) The bacterial glycocalyx in nature and disease. Annu Rev Microbiol 35:299–324Google Scholar
  8. 8.
    Costeron JW, Cheng KJ, Geesey GG (1987) Bacterial biofilms in nature and disease. Annu Rev Microbiol 41:435–464Google Scholar
  9. 9.
    Crossin KL, Hoffman S, Grumet M, Thiery JP, Edelman GM (1986) Site-restricted expression of eytotactin during development of the chicken embryo. J Cell Biol 102:1917–1930Google Scholar
  10. 10.
    Erickson HP, Bourdon MA (1989) Tenascin: an extracellular matrix protein present in specialized embryonic tissues and in tumors. Annu Rev Cell Biol 5:71–92Google Scholar
  11. 11.
    ffrench-Constant C, Hynes RO (1988) Patterns of fibronectin gene expression and splicing during cell migration in chicken embryos. Development 104:369–382Google Scholar
  12. 12.
    ffrench-Constant C, Van der Water L, Dvosdrak HF, Hynes RO (1989) Reappearance of an embryonic pattern of fbronectin splicing during wound healing in the adult rat. J Cell Biol 109:903–914Google Scholar
  13. 13.
    Flock J-I, Fröman G, Jönsson K, Guss B, Signäs C, Nilsson B, Raucci G, Höök M, Wadström T, Lindberg M (1987) Cloning and expression of the gene for a fibronectin-binding protein from Staphylococcus aureus. EMBO J 6:2351–2357Google Scholar
  14. 14.
    Fröman G, Switalski LM, Speziale P, Höök M (1987) Isolation and characterization of a fibronectin-binding receptor fromStaphylococcus aureus. J Biol Chem 262:6564–6571Google Scholar
  15. 15.
    Gristina AG (1987) Biomaterial-centered infection: microbial adhesion versus tissue integration. Science 237:1588–1595Google Scholar
  16. 16.
    Herrmann M, Vaudaux PE, Pittet D, Auchenthaler R, Lew PD, Schumacher-Perdreau F, Peters G, Waldvogel FA (1988) Fibronectin, fibrinogen, and laminin act as mediators of adherence of clinical Staphylococcal isolates to foreign material. J Infect Dis 158:693–710Google Scholar
  17. 17.
    Herrmann M, Suchard SJ, Bower LA, Waldvogel FA, Lew PD (1991) Thrombospondin binds toStaphylococcus aureus and promotes staphylococcal adherence to surfaces. Infect Immun 59:279–288Google Scholar
  18. 18.
    Hoffman S, Crossin KL, Edelman GM (1988) Molecular forms, binding functions, and developmental patterns of cytotactin and cytotactin-binding proteoglycan, and interactive pair of extracellular matrix molecules. J Cell Biol 106:519–532Google Scholar
  19. 19.
    Holland SP, Pulido JS, Miller D, Ellis B, Alfonso E, Scott M, Costeron JW (1991) Biofilm and scleral buckle-associated infections. A mechanism for persistence. Ophthalmology 98:933–938Google Scholar
  20. 20.
    Höök M, Switalski LM, Wadström T, Lindberg M (1989) Interactions of pathogenic microorganisms with fibronectin.Fibronectin Academic Press Inc New YorkGoogle Scholar
  21. 21.
    Howeedy AA, Virtanen I, Laitinen L, Gould NS, Koukoulis G, Gould VE (1990) Differential distribution of tenascin in the normal, hyperplastic, and neoplastic breast. Lab Invest 63:798–806Google Scholar
  22. 22.
    Hynes RO (1985) Molecular biology of fibronectin. Ann Rev Cell Biol 1:67–90Google Scholar
  23. 23.
    Hynes RO (1986) Fibronectins. Sci Am 254:32–41Google Scholar
  24. 24.
    Immonen I, Tervo K, Virtanen I, Laatikainen L, Tervo T (1991) Immunohistochemical demonstration of cellular fibronectin and tenascin in human epiretinal membranes. Acta Ophthalmol 69:466–471Google Scholar
  25. 25.
    Inaguma Y, Kusakabe M, Mackie EJ, Pearson CA, Chiquet-Ehrismann R, Sakakura T (1988) Epithelial induction of stromal tenascin in mouse mammary gland: from embryogenesis to carcinogenesis. Dev Biol 128:245–255Google Scholar
  26. 26.
    Kivelä T, Tarkkanen A, Virtanen I (1989) Synaptophysin in human retina and retinoblastoma. An immunohistochemical and western blotting study. Invest Ophthalmol Vis Sci 30:212–219Google Scholar
  27. 27.
    Koukouls GK, Gould VE, Bhattacharyya A, Gould JE, Howeedy AA, Virtanen I (1991) Tenascin in normal, reactive, hyperplastic, and neoplastic tissues: biologic and pathologic implications. Human Pathol 22:636–643Google Scholar
  28. 28.
    Kuusela P (1978) Fibronectin binds toStaphylococcus aureus. Nature 276:718–720Google Scholar
  29. 29.
    Kuypers JM, Proctor RA (1989) Reduced adherence to traumatized rat heart valves by a low-fibronectin-binding mutant ofStaphylococcus aureus. Infect Immun 57:2306–2312Google Scholar
  30. 30.
    Lightner VA, Gumkowski F, Bigner DD, Erickson HP (1989) Tenascin/hexabrachion in human skin: biochemical identification and localization by light and electron microscopy. J Cell Biol 108:2483–2493Google Scholar
  31. 31.
    Lotz MM, Burdsal CA, Erickson HP, McClay DR (1989) Cell adhesion to fibronectin and tenascin: Quantitative measurements of initial binding and subsequent strengthening response. J Cell Biol 109:1795–1805Google Scholar
  32. 32.
    Mackie EJ, Halfter W, Liverani D (1988) Induction of tenascin in healing wounds. J Cell Biol 107:2757–2767Google Scholar
  33. 33.
    Mackie EJ, Thesleff I, Chiquet-Ehrismann R (1987) Tenascin is associated with chondrogenic and osteogenic differentiation in vivo and promotes chondrogenesis in vitro. J Cell Biol 105:2569–2579Google Scholar
  34. 34.
    Norton PA, Hynes RO (1987) Alternative splicing of chicken fibronectin in embryos and in normal and transformed cells. Mol Cell Biol 7:4297–4307Google Scholar
  35. 35.
    Proctor RA, Christman G, Mosher DF (1982) Fibronectin binding toStaphylococcus aureus. J Biol Chem 257:14788–14794Google Scholar
  36. 36.
    Proctor RA, Christman G, Mosher DF (1984) Fibronectin-induced agglutination ofStaphylococcus aureus correlates with invasiveness. J Lab Clin Med 104:455–469Google Scholar
  37. 37.
    Quie PG, Belani KK (1987) Coagulase-negative staphylococcal adherence and persistence. J Infect Dis 157:543–547Google Scholar
  38. 38.
    Raja RH, Raucci G, Hook M (1990) Peptide analogs to fibronectin receptor inhibit attachment ofStaphylococcus aureus to fibronectin-containing substrates. Infect Immun 58:2593–2598Google Scholar
  39. 39.
    Ruoslahti E (1988) Fibronectin and its receptors. Annu Rev Biochem 57:375–413Google Scholar
  40. 40.
    Ruoslahti E (1991) Integrins. J Clin Invest 87:1–5Google Scholar
  41. 41.
    Shields CL, Shields JA, Eagle RC, De Potter P (1991) Histopathologic evidence of fibrovascular ingrowth four weeks after placement of the hydroxyapatite orbital implant. Am J Ophthalmol 111:363–366Google Scholar
  42. 42.
    Signäs C, Raucci G, Musson K, Lindgren P-E, Anantharamaiah GM, Höök M, Lindberg M (1989) Nucleotide sequence of the gene for a fibronectin-binding protein fromStaphylococcus aureus: Use of this peptide sequence in the synthesis of biologically active peptides. Proc Natl Acad Sci USA 86:699–703Google Scholar
  43. 43.
    Skalli O, Ropraz P, Trzeceak A, Benzonana G, Gillessen D, Gabbiani G (1986) A monoclonal antibody against a-smooth muscle actin: a new probe for smooth muscle differentiation. J Cell Biol 106:2787–2796Google Scholar
  44. 44.
    Speaker MG, Milch FA, Shah MK, Eisner W, Kreiswirth BN (1991) Role of external bacterial flora in the pathogenesis of acute postoperative endophthalmitis. Ophthalmology 98:639–650Google Scholar
  45. 45.
    Tervo K, Tervo T, van Setten G-B, Tarkkanen A, Virtanen I (1989) Demonstration of tenascin-like immunoreactivity in rabbit corneal wounds. Acta Ophthalmol 67:347–350Google Scholar
  46. 46.
    Vartio T, Laitinen L, Närvänen O, Cutolo M, Thornell L-E, Zardi L, Virtanen I (1987) Differential expression of the ED sequence-containing form of cellular fibronectin in embryonic and adult human tissues. J Cell Sci 88:419–430Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Taru Päällysaho
    • 1
  • Kaarina Tervo
    • 1
  • Tero Kivelä
    • 2
  • Ismo Virtanen
    • 1
  • Ahti Tarkkanen
    • 2
  • Timo Tervo
    • 2
  1. 1.Departments of AnatomyUniversity of HelsinkiHelsinki
  2. 2.OphthalmologyUniversity of HelsinkiHelsinkiFinland

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