Journal of Clinical Immunology

, Volume 6, Issue 2, pp 114–120

An analysis of the cellular requirements for the production of soluble interleukin-2 receptorsin vitro

  • David L. Nelson
  • Laurence A. Rubin
  • Carole C. Kurman
  • Mary E. Fritz
  • Bernard Boutin
Original Articles

Abstract

Following activationin vitro, peripheral blood mononuclear cells (PBMC) express cell-associated interleukin-2 receptors (IL-2R) and also release soluble IL-2R into culture supernatants. The present studies were undertaken to define which normal cells were responsible for the release of soluble IL-2Rin vitro. Both cell-associated and soluble IL-2R were quantitatively measured with a “sandwich” enzyme-linked immunoassay employing two monoclonal antibodies. PBMC were separated into populations of surface immunoglobulin-negative cells (T cells and monocytes) and surface immunoglobulin-positive cells (B cells and monocytes), and the T-cell population was further separated into OKT4-positive (OKT4+) cells and OKT4-negative (OKT4) cells. Following activation with phytohemagglutinin, pokeweed mitogen, and the monoclonal antibody OKT3, large amounts of soluble IL-2R were released by PBMC, unseparated T cells, OKT4+ T cells, and OKT4 T cells. The population containing B cells and monocytes made small but readily detectable amounts of soluble IL-2R when stimulated with these T-cell mitogens; likely the result of contaminating T cells in the population. However, when highly purified B cells were stimulated withStaphylococcus aureus Cowan and recombinant IL-2, they also released small amounts of soluble IL-2R. The release of soluble IL-2R by T cells appeared monocyte dependent when OKT3, but not phytohemagglutinin, was employed for activation, and monocytes themselves released no detectable IL-2R under the conditions employed. These studies define the cellular requirements for the release of soluble IL-2Rin vitro and demonstrate that such receptors are released by B cells, T cells, and both OKT4+ and OKT4 T-cell subsets.

Key words

Interleukin-2 interleukin-2 receptor lymphocyte subsets lymphocyte activation 

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Copyright information

© Plenum Publishing Corporation 1986

Authors and Affiliations

  • David L. Nelson
    • 1
  • Laurence A. Rubin
    • 1
  • Carole C. Kurman
    • 1
  • Mary E. Fritz
    • 1
  • Bernard Boutin
    • 1
  1. 1.Immunophysiology Section, Metabolism Branch, National Cancer InstituteNational Institutes of HealthBethesda

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