Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Immunodeficient mice xenografted with human lymphoid cells: New models forin vivo studies of human immunobiology and infectious diseases


This review article deals with the transfer of human lymphoid or hematopoietic tissues to severely immunodeficient mice to create new small animal models for the study of human biology and disease. The degree of functional reconstitution in the three current models is discussed. SCID mice with human grafts have been infected with human immunodeficiency virus (HIV) to generate a small animal model for AIDS research. Epstein-Barr virus (EBV)-related lymphoproliferative disorders can also be modeled by the transfer of adult peripheral blood mononuclear cells to SCID mice.

This is a preview of subscription content, log in to check access.


  1. 1.

    Fogh J, Fogh JM, Orfeo T: One hundred and twenty seven cultured tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59:2216–2221, 1977

  2. 2.

    Mosier DE, Gulizia RJ, Baird SM, Wilson DB: Transfer of a functional human immune system to mice with severe combined immunodeficiency. Nature 335:256–259, 1988

  3. 3.

    McCune JM, Namikawa R, Kaneshima H, Schultz LD, Lieberman M, Weissman IL: The SCID-hu mouse: Murine model for the analysis of human hematolymphoid differentiation and function. Science 241:1632–1639, 1988

  4. 4.

    Kamel-Reid S, Dick JE: Engraftment of immune-deficient mice with human hematopoietic stem cells. Science 242:1706–1709, 1988

  5. 5.

    Namikawa R, Kaneshima H, Lieberman M, Weissman IL, McCune JM: Infection of the SCID-hu mouse by HIV-1. Science 242:1684–1687, 1988

  6. 6.

    Mosier DE, Gulizia RJ, Spector SA, Spector DH, Baird SM, Wilson DB: Human immunodeficiency virus infection of SCID mice reconstituted with human peripheral blood leucocytes (submitted for publication)

  7. 7.

    Krams SM, Dorschkind K, Gerschwin ME: Generation of biliary lesions after transfer of human lymphocytes into severe combined immunodeficient (SCID) mice. J Exp Med 170:1919–1930, 1989

  8. 8.

    Bosma GC, Custer RP, Bosma MJ: A severe combined immunodeficiency mutation in the mouse. Nature 301:527–530, 1983

  9. 9.

    Schuler W, Weiler, IJ, Schuler A, Phillips RA, Rosenberg N, Mak TW, Kearney JF, Perry RP, Bosma MJ: Rearrangement of antigen receptor genes is defective in mice with severe combined immune deficiency. Cell 46:963–972, 1986

  10. 10.

    Dorschkind K, Pollack SB, Bosma MJ, Phillips RA: Natural killer (NK) cells are present in mice with severe combined immunodeficiency (SCID). J Immunol 134:3798–3801, 1985

  11. 11.

    Andriole GL, Mule JJ, Hansen CT, Linehan WM, Rosenberg SA: Evidence that lymphokine-activated killer cells and natural killer cells are distinct based on an analysis of congenitally immunodeficient mice. J Immunol 135:2911–2913, 1985

  12. 12.

    Kamel-Reid S, Letarte M, Sirard C, Doedens M, Grunberger T, Fulop G, Freedman MH, Phillips RA, Dick JE: A model of human acute lymphoblastic leukemia in immune-deficient SCID mice. Science 246:1597–1600, 1989

  13. 13.

    Mosier DE, Gulizia RJ, Baird SM, Wilson DB: Scientific correspondence. Nature 338:211, 1989

  14. 14.

    Mosier DE, Gulizia RJ, Wilson DB, Baird SM, Spector SA, Spector DH, Richman DD, Fox RI, Kipps TJ: Elements of the human immune system: Studies of mature lymphoid cells following xenotransplantation to SCID mice.In Progress in Immunology VII, F Melcherset al. (eds). Springer-Verlag, Berlin, 1989, pp 1264–1271

  15. 15.

    McCune JM, Namikawa R, Shih C-C, Rabin L, Kaneshima H: Suppression of HIV infection in AZT-treated SCID-hu mice. Science 247:564–566, 1990

  16. 16.

    Koyanagi Y, Miles S, Mitsuyasu, RT, Merrill JE, Vinters HV, Chen ISY: Dual infection of the central nervous system by AIDS viruses with distinct cellular tropisms. Science 236:819–822, 1987

  17. 17.

    Penn I: Malignant lymphomas in organ transplant recipients. Transplant Proc 13:736–8, 1981

  18. 18.

    Birx DL, Redfield RR, Tosata G: Defective regulation of Epstein-Barr virus infection in patients with acquired immunodeficiency syndrome (AIDS) or AIDS-related disorders. N Engl J Med 14:8711–8718, 1986

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Mosier, D.E. Immunodeficient mice xenografted with human lymphoid cells: New models forin vivo studies of human immunobiology and infectious diseases. J Clin Immunol 10, 185–191 (1990). https://doi.org/10.1007/BF00918650

Download citation

Key words

  • Severe combined immune deficient (SCID) mice
  • human xenografts
  • human immunodeficiency virus (HIV) infection
  • Epstein-Barr virus (EBV) lymphomagenesis