Journal of Clinical Immunology

, Volume 12, Issue 4, pp 259–265 | Cite as

Conjunctival epithelial cells from patients with Sjögren's syndrome inappropriately express major histocompatibility complex molecules, La(SSB) antigen, and heat-shock proteins

  • Dimitri I. Yannopoulos
  • Stéphane Roncin
  • Armelle Lamour
  • Yvon L. Pennec
  • Haralampos M. Moutsopoulos
  • Pierre Youinou
Original Articles


The La(SSB) antigen has been detected within the cytoplasm and on the membrane of conjunctival cells (CC) from patients with Sjögren's syndrome, whereas it was weakly expressed in the nucleus of normal cells. The diseased CC were shown to overproduce major histocompatibility complex (MHC) class I antigens and express MHC class II antigens. Anti-heat-shock protein monoclonal antibody bound to the cell membrane in patients but not in normal controls.

Key words

Sjögren's syndrome La(SSB) antigen epithelial cells 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Youinou P, Moutsopoulos HM, Pennec YL: Clinical features of Sjögren's syndrome. Curr Opin Rheumatol 2:687–690, 1990PubMedGoogle Scholar
  2. 2.
    Moutsopoulos HM, Youinou P: New developments in Sjögren's syndrome. Curr Opin Rheumatol 3:815–822, 1991PubMedGoogle Scholar
  3. 3.
    Green JE, Hinrichs SH, Vogel J, Jay G: Exocrinopathy resembling Sjögren's syndrome in HTLV-1 tax transgenic mice. Nature 341:72–74, 1989PubMedGoogle Scholar
  4. 4.
    Baboonian C, Venables PJW, Booth J, Williams DG, Roffe LM, Maini RN: Virus infection induces redistribution and membrane localization of the nuclear antigen La(SSB): A possible mechanism for autoimmunity. Clin Exp Immunol 78:454–459, 1989PubMedGoogle Scholar
  5. 5.
    Moutsopoulos HM, Hooks JJ, Chan CC, Dalavanga YA, Skopouli FN, Detrick B: HLA-Dtr expression by labial monir salivary gland tissues in Sjögren's syndrome. Ann Rheum Dis 45:677–683, 1986PubMedGoogle Scholar
  6. 6.
    Vanbuskirk A, Crump BL, Margoliash E, Pierce SK: A peptide binding protein having a role in antigen presentation is a member of the hsp 70 heat shock family. J Exp Med 170:1799–1809, 1989PubMedGoogle Scholar
  7. 7.
    Skopouli FF, Drosos AA, Papaioannou T, Moutsopoulos HM: Preliminary diagnostic criteria for Sjögren's syndrome. Scand J Rheumatol Suppl 61:22–25, 1986PubMedGoogle Scholar
  8. 8.
    Egbert PR, Lauber S, Maurice DM: A simple conjunctival biopsy. Am J Ophtalmol 84:789–801, 1977Google Scholar
  9. 9.
    Smith PR, Williams DG, Venables PJW, Maini RN: Monoclonal antibodies to the Sjögren's syndrome associated antigen SSB(La). J Immunol Methods 77:63–76, 1985PubMedGoogle Scholar
  10. 10.
    Riehl RM, Sullivan WP, Vroman BT, Bauer VJ, Pearson GR, Toft DO: Immunological evidence that the nonhormone binding component of avian steroid receptors exists in a wide range of tissues and species. Biochemistry 24:6586–6591, 1985PubMedGoogle Scholar
  11. 11.
    Le Feber WP, Norris DA, Ryan SR, Huff JC, Lee LA, Kubo M, Boyce ST, Kotzin BL, Weston WL: Ultraviolet light induces binding of antibodies to selected nuclear antigens on cultured human keratinocytes. J Clin Invest 74:1545–1551, 1984PubMedGoogle Scholar
  12. 12.
    Bachmann M, Chang S, Bernd A, Mayet W, Meyer Zum Büschenfelde KH, Müller WEG: Translocation of the nuclear autoantigen La to cell surface, Assembly and disassembly with the extracellular matrix. Autoimmunity 9:99–107, 1991PubMedGoogle Scholar
  13. 13.
    Baudowin C, Fredj-Reygrobellet D, Lapalus P, Gastaud P: Immunopathological study on proliferative diabetic retinopathy. Am J Opthalmol 105:383–386, 1988Google Scholar
  14. 14.
    Scalzo AA, Anders EM: Influenza viruses as lymphocyte mitogens. II. Role of I-E molecules in B cell mitogenesis by influenza viruses of the H2 and H6 subtypes. J Immunol 135:3524–3528, 1985PubMedGoogle Scholar
  15. 15.
    La Thangue NB, Latchman DS: A cellular protein related to heat-shock protein 90 accumulates during herpes simplex virus infection and is overexpressed in transformed cells. Exp Cell Res 178:169–179, 1988PubMedGoogle Scholar
  16. 16.
    Ferris DK, Harrel Bellan A, Morimoto RI, Welch WH, Farrar WL: Mitogen and lymphokine stimulation of heat-shock protein in T lymphocytes, Proc Natl Acad Sci USA 85:3850–3854, 1988PubMedGoogle Scholar
  17. 17.
    Norton PM, Isenberg DA, Latchman DS: Elevated levels of the 90kD heat-shock protein in a proportion of SLE patients with active disease J Autoimmun 2:187–195, 1989Google Scholar
  18. 18.
    Minota S, Cameron B, Welch WJ, Winfield JB: Autoantibodies to the constitutive 73-kD member of the hsp 70 family of heat-shock proteins in systemic lupus erythematosus. J Exp Med 168:1475–1480, 1988PubMedGoogle Scholar
  19. 19.
    Jarjour WN, Jeffries BD, Davis JS IV, Welch WJ, Mimura T, Winfield JB: Autoantibodies to human stress proteins. A survey of various rheumatic and other inflammatory diseases. Arth Rheum 34:1133–1137, 1991Google Scholar
  20. 20.
    Blaschek MA, Pennec YL, Simitzis AM, Jouquan J, Kerdraon G, Youinou P: Anti-Golgi complex autoantibodies in patients with primary Sjögren's syndrome. Scand J Rheumatol 17:291–296, 1988PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1992

Authors and Affiliations

  • Dimitri I. Yannopoulos
    • 1
  • Stéphane Roncin
    • 2
  • Armelle Lamour
    • 1
  • Yvon L. Pennec
    • 3
  • Haralampos M. Moutsopoulos
    • 1
  • Pierre Youinou
    • 1
  1. 1.Laboratory of ImmunologyBrest University Medical School HospitalBrest CedexFrance
  2. 2.Unit of OphthalmologyBrest University Medical SchoolBrestFrance
  3. 3.Department of Internal MedicineBrest University Medical SchoolBrestFrance

Personalised recommendations