Inflammation

, Volume 2, Issue 3, pp 191–197 | Cite as

Metabolism of arachidonic acid in rabbit alveolar macrophages

  • Saura Sahu
  • William S. Lynn
Article

Abstract

Rabbit alveolar macrophages incorporated radioactive arachidonic acid primarily into phosphatidylcholine, phosphatidylethanolamine, sphingomyelin, and triglycerides. When these cells, so labelled, were washed and treated with polystyrene beads, there was a distinct and reproducible decrease in the radioactivity of phosphatidylcholine, phosphatidylethanolamine, and triglyceerides. This decrease was accounted for by the appearance of two major prostaglandins, PGE2 and PGF2a, and uncharacterized hydroxy fatty acids, which may be 12-hydroxy-5,8,10,14-eicosatetraenoic acid, 5-hydroxy-6,8,11, 14-eicosatetraenoic acid, and/or 8-hydroxy-9,11,14-eicosatrienoic acid.

Keywords

Public Health Internal Medicine Triglyceride Prostaglandin Polystyrene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Anggard, E., andB. Samuelson. 1965. Biosynthesis of prostaglandin from arachidonic acid in guinea pig lung.J. Biol. Chem. 240:3518–3521.Google Scholar
  2. 2.
    Dayer, J., S.M. Krane, R.G. Russel, andD.R. Robinson. 1976. Production of collagenase and prostaglandins by isolated adherent rheumatoid synovial cells.Proc. Natl. Acad. Sci. U.S.A. 73:945–949.Google Scholar
  3. 3.
    Smith, J.B., C. Ingerman, J. Kocsis, andM. Silver. 1973. Formation of prostaglandins during aggregation of human blood platelets.J. Clin. Invest. 52:965–969.Google Scholar
  4. 4.
    Samuelson, B., E. Granstrom, K. Green, M. Hamberg, andS. Hammarstrom. 1975. Prostaglandins.Annu. Rev. Biochem. 44:669–695.Google Scholar
  5. 5.
    Higgs, G.A., and L.J.F.Youlten. 1972. Prostaglandin production by rabbit peritoneal polymorphonuclear leukocytes.Br. J. Pharmacol. 44:330p.Google Scholar
  6. 6.
    Elsbach, P., andM.A. Rizack. 1963. Acid lipase and phospholipase activity in homogenates of rabbit polymorphonuclear leukocytes.Am. J. Physiol. 205:1154–1158.Google Scholar
  7. 7.
    Stossel, T.P., T.D. Pollard, R.J. Mason, andM. Vaughan. 1971. Isolation and properties of phagocytic vesicles for polymorphonuclear leukocytes.J. Clin. Invest. 50:1745–1757.Google Scholar
  8. 8.
    Marcus, A.J., H.L. Ulman, andL.B. Safer. 1969. Lipid composition of subcellular particles of human blood platelets.J. Lipid Res. 10:108–114.Google Scholar
  9. 9.
    Bills, T.K., J.B. Smith, andM.J. Silver. 1976. Metabolism of arachidonic acid by human platelets.Biochim. Biophys. Acta 424:303–314.Google Scholar
  10. 10.
    Mason, R.J., T.P. Stossel, andM. Vaughan. 1972. Lipids of alveolar macrophages, polymorphonuclear leukocytes, and their phagocytic vesicles.J. Clin. Invest. 51:2399–2407.Google Scholar
  11. 11.
    Stossel, T.P., R.J. Mason, T.D. Pollard, andM. Vaughan, 1972. Isolation and properties of phagocytic vesicles. II. Alveolar macrophages.J. Clin. Invest. 51:604–614.Google Scholar
  12. 12.
    Elsbach, P. 1966. Phospholipid metabolism by phagocytic cells.Biochim. Biophys. Acta 125:510–524.Google Scholar
  13. 13.
    Myrvick, Q.N., E.S. Leake, andB. Paris. 1961. Studies on alveolar macrophages from normal rabbits: A technique to procure them in high purity.J. Immunol. 86:128–132.Google Scholar
  14. 14.
    Bligh, E.G., andW.J. Dyer. 1959. A rapid method of total lipid extraction and purification.Can. J. Physiol. 37:911–917.Google Scholar
  15. 15.
    Sahu, S., R.P. DiAugustine, andW.S. Lynn. 1976. Lipids found in pulmonary lavage of patients with alveolar proteinosis and in rabbit lung lamellar organelles.Am. Rev. Resp. Dis. 114: 177–185.Google Scholar
  16. 16.
    Nugteren, D.H. 1975. Arachidonate lipoxygenase in blood platelets.Biochim. Biophys. Acta 380:299–307.Google Scholar
  17. 17.
    McGarrity, J.T., andJ.B. Armstrong. 1975. Effects of salt on phospholipid fatty acid composition inE. coli. Biochim. Biophys. Acta 398:258–264.Google Scholar
  18. 18.
    Ohuchi, K., H. Sato, andS. Rsurufuji. 1976. Content of prostaglandins E and F in exudate of carrageenin granuloma of rats.Biochim. Biophys. Acta 426:439–448.Google Scholar
  19. 19.
    Bergeat, P., M. Hamberg, andB. Samuelson, 1976. Transformation of arachidonic acid by rabbit polymorphonuclear leukocytes.J. Biol. Chem. 251:7816–7820.Google Scholar
  20. 20.
    Flower, R.J. 1974. Drugs which inhibit prostaglandin biosynthesis.Pharmacol. Rev. 26:33–67.Google Scholar

Copyright information

© Plenum Publishing Corporation 1977

Authors and Affiliations

  • Saura Sahu
    • 1
  • William S. Lynn
    • 1
  1. 1.Departments of Biochemistry and MedicineDuke University Medical CenterDurham

Personalised recommendations