Journal of Clinical Immunology

, Volume 11, Issue 4, pp 205–212 | Cite as

Analysis of function-associated receptor molecules on peripheral blood and synovial fluid granulocytes from patients with rheumatoid and reactive arthritis

  • Thomas Felzmann
  • Stephen Gadd
  • Otto Majdic
  • Dieter Maurer
  • Peter Petera
  • Josef Smolen
  • Walter Knapp
Original Articles
Accepted:

Abstract

In this study we report the expression pattern of 13 different function-associated surface molecules on synovial fluid and peripheral blood granulocytes from rheumatoid and reactive arthritis patients. We found increased expression of the complement receptors 1 (CD35) and 3 (CD11b) and of the activation-associated antigens CD67, CD24, and M5 on synovial fluid granulocytes from rheumatoid and/or reactive arthritis patients compared to autologous peripheral blood granulocytes. In addition, synovial fluid granulocytes expressed IgG Fc receptor 1 (CD64) and complement receptor 4 (CD11c), neither of which can be found on peripheral blood granulocytes. Peripheral blood granulocytes from rheumatoid and reactive arthritis patients expressed higher levels of leucocyte function-associated antigen 1 (CD11a) and of the membrane proteins CD31, CD24, M5, and M6 compared to peripheral blood granulocytes from healthy controls and patients with degenerative joint disease. No significant differences in the expression of any of the molecules studied could be observed between cells from rheumatoid and cells from reactive arthritis patients, suggesting a similar activation process for granulocytes in these two diseases.

Key words

Fc receptors complement receptors adhesion molecules granulocyte activation flow cytometry 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Socinski MA, Cannistra SA, Sullivan R, Elias A, Antman K, Schnipper L, Griffin JD: Granulocyte-macrophage colony-stimulating factor induces the expression of the CD11b surface adhesion molecule on human granulocytes in vivo. Blood 72:691–697, 1988Google Scholar
  2. 2.
    Petroni KC, Shen L, Guyre PM: Modulation of human polymorphonuclear leucocyte IgG Fc receptors and Fc receptor mediated functions by IFN-γ and glucocorticoids. J Immunol 140:3467–3472, 1988Google Scholar
  3. 3.
    Shen L, Guyre PM, Fanger MW: Polymorphonuclear leucocyte function triggered through the high affinity Fc receptor for monomeric IgG. J Immunol 139:534–538, 1987Google Scholar
  4. 4.
    Lo SK, Van Seventer GA, Levin SM, Wright SD: Two leucocyte receptors (CD11a/CD18 and CD11b/CD18) mediate transient adhesion to endothelium by binding to different ligands. J Immunol 143:3325–3329, 1989Google Scholar
  5. 5.
    Paccaud JP, Schifferli JA, Baggiolini M: Nap-1/IL-8 induces upregulation of CR1 receptors in human neutrophil leukocytes. Biochem Biophys Res Commun 166:187–192, 1990Google Scholar
  6. 6.
    Fearon DT, Collins LA: Increased expression of C3b receptors on polymorphonuclear leucocytes induced by chemotactic factors and by purification procedures. J Immunol 130:370–375, 1983Google Scholar
  7. 7.
    Werfel T, Koch G, Götze O: Myeloid antigens are increased on peripheral granulocytes and monocytes but not on lymphocytes in response to C5a.In Leucocyte Typing IV. W Knapp (eds). Oxford, Oxford University Press, 1989, pp 900–901Google Scholar
  8. 8.
    Wright SD, Levin SM, Jong MTC, Chad Z, Kabbash LG: CR3 (CD11b/CD18) expresses one binding site for Arg-Gly-Asp-Containing peptides and a second site for bacterial lipopolysaccharide. J Exp Med 169:175–183, 1989Google Scholar
  9. 9.
    Fischer GF, Majdic O, Knapp W: Altered expression of surface antigens after stimulation of human granulocytes.In Leucocyte Typing III, A. McMichael (eds). Oxford, Oxford University Press, 1987, pp 698–702Google Scholar
  10. 10.
    Tetteroo PAT, Bos MJE, Visser FJ, van dem Borne AEGKr: Neutrophil activation detected by monoclonal antibodies. J Immunol 136:3427–3432, 1986Google Scholar
  11. 11.
    Gadd SJ, Majdic O, Kasinrerk W, Stockinger H, Maurer D, Eher R, Knapp W: M5, a phosphoinositol linked myelomonocytic activation-associated antigen. Clin Exp Immunol 80:252–256, 1990Google Scholar
  12. 12.
    Fischer GF, Majdic O, Gadd S, Knapp W: Signal transduction in lymphocytic and myeloid cells via CD24, a new member of phosphoinositol-anchored membrane molecules. J Immunol 144:638–641, 1990Google Scholar
  13. 13.
    Stockinger H, Gadd SJ, Eher R, Majdic O, Kasinrerk W, Strass B, Schreiber W, Schnabl E, Knapp W: Molecular characterisation and functional analysis of the leucocyte surface protein CD31. J Immunol 145:3889–3897, 1990Google Scholar
  14. 14.
    Konttinen YT, Honkanen VEA: Future trends in the treatment of rheumatoid arthritis in the light of current etiopathogenetic theories. Scand J Rheumatol Suppl 74:7–17, 1988Google Scholar
  15. 15.
    Emery P, Lopez AF, Burns BF, Vadas MA: Synovial fluid neutrophils of patients with rheumatoid arthritis have membrane antigen changes that reflect activation. Ann Rheum Dis 47:34–39, 1988Google Scholar
  16. 16.
    Arnett FC, Edworthy SM, Bloch DA, McShane DJ, Fries JF, Cooper NS, Healeey LA, Kaplan SR, Liang MH, Luthra HS, Jr TA Medgsger, Mitchell DM, Neustadt DH, Pinals RS, Schaller JG, Sharp JT, Wilder RL, Hunder GG: The American rheumatism association 1987 revised criteria for the classification of rheumatoid arthritis. Arth Rheum 3:315–325, 1988Google Scholar
  17. 17.
    Steinbroker O, Traeger CH, Batterman RC: Therapeutic criteria for rheumatoid arthritis. JAMA 140:659–662, 1949Google Scholar
  18. 18.
    Boyum A: Isolation of leucocytes from human blood and bone marrow. Scand J Clin Invest 21 (Suppl 97):77–81, 1968Google Scholar
  19. 19.
    Gadd SJ, Ashman LK: Binding of mouse monoclonal antibodies to human leukaemic cells via the Fc receptor: A possible source of “false positive” reactions in specificity screening. Clin Exp Immunol 54:811–816, 1983Google Scholar
  20. 20.
    Anderson CL, Guyre PM, Whitin JC, Ryan DH, Looney RJ, Fanger MW: Monoclonal antibodies to Fc receptors for IgG on human mononuclear phagocytes. J Biol Chem 261:12856–12864, 1986Google Scholar
  21. 21.
    Looney RJ, Abraham GN, Anderson CL: Human Monocyte and U937 Cells bear two distinct Fc receptors for IgG. J Immunol 136:1641–1647, 1986Google Scholar
  22. 22.
    Gerdes J, Naiem M, Mason DY, Stein H: Human complement (C3b) receptors defined by a mouse monoclonal antibody. Immunology 45:645–653, 1982Google Scholar
  23. 23.
    Hildreth JEK, Gotch FM, Hildreth PDK, McMichael AJ: A human lymphocyte-associated antigen involved in cell-mediated lympholysis. Eur J Immunol 13:202–208, 1983Google Scholar
  24. 24.
    Knapp W, Majdic O, Stockinger H, Bettelheim P, Liszka K, Köller U, Peschel C: Monoclonal antibodies to human myelomonocyte differentiation antigens in the diagnosis of acute myeloid leukemia. Med Oncol Tumor Pharmacother 4:257–262, 1984Google Scholar
  25. 25.
    Maynes BL, Dalzell JG, Hogg N, Ross GD: Neutrophil and monocyte cell surface p150/95 has iC3b-receptor (CR4) activity resembling CR3. J Clin Invest 82:640–651, 1988Google Scholar
  26. 26.
    Knapp W, Majdic O, Bettelheim P, Liszka K, Aberer W, Stingl G: Typing of leukemic cells with monoclonal antibodies. Ann NY Acad Sci 420:251–260, 1983Google Scholar
  27. 27.
    Majdic O, Liszka K, Lutz D, Knapp W: Myeloid differentiation antigen defined by monoclonal antibody. Blood 58:1127–1133, 1981Google Scholar
  28. 28.
    Solberg HE: Establishment and use of reference values.In Textbook of Clinical Chemistry, NW Tietz (eds). Philadelphia, WB Saunders, 1986, pp 356–385Google Scholar
  29. 29.
    Hogg N: The leucocyte integrins. Immunol Today 10:111–114, 1989Google Scholar
  30. 30.
    Waldman H, Cobbold S: Workshop N2—The LFA family and other nonlineage antigens.In Leucocyte Typing III, AJ McMichael (eds). Oxford, Oxford University Press, 1987, p 829Google Scholar
  31. 31.
    Unkeless JC, Scigliano E, Freedman VH: Structure and function of human and murine receptors for IgG. Annu Rev Immunol 6:251–281, 1988Google Scholar
  32. 32.
    Ross GD, Medoff ME: Membrane complement receptors specific for bound fragments of C3. Adv Immunol 37:217–235, 1985Google Scholar
  33. 33.
    Rosen H, Gordon S: Monoclonal antibody to the murine type 3 complement receptor inhibits adhesion of myelomonocytic cells in vitro and inflammatory cell recruitment in vivo. J Exp Med 166:1685–1701, 1987Google Scholar
  34. 34.
    Fearon DT, Collins LA: Increased expression of C3b receptors on polymorphonuclear leukocytes induced by chemotactic factors and by purification procedures. J Immunol 130:370–375, 1983Google Scholar
  35. 35.
    Hed J, Berg O, Forslid J, Hallden G, Lärka-Rafner G: The expression of CR1 and CR3 on non-modulated and modulated granulocytes of healthy blood donors as measured by flow cytofluorometry. Scand J Immunol 28:339–344, 1988Google Scholar
  36. 36.
    Fanger MW, Shen L, Graziano RF, Guyre PM: Cytotoxicity mediated by human Fc receptors for IgG. Immunol Today 10:92–99, 1989Google Scholar
  37. 37.
    Hessian PA, Highton J, Palmer DG: Quantification of macrophage cell surface molecules in rheumatoid arthritis. Clin Exp Immunol 77:47–51, 1989Google Scholar
  38. 38.
    Stockinger H: Cluster Report: CD67.In Leucocyte typing IV, W Knapp (eds). Oxford University Press /Oxford, UK, 1989, pp 840–841Google Scholar
  39. 39.
    Newman PJ, Berndt MC, Gorski J, White II GC, Lyman S, Paddock C, Muller WA: PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily. Science 247:1219–1222, 1990Google Scholar

Copyright information

© Plenum Publishing Corporation 1991

Authors and Affiliations

  • Thomas Felzmann
    • 1
  • Stephen Gadd
    • 1
  • Otto Majdic
    • 1
  • Dieter Maurer
    • 1
  • Peter Petera
    • 2
  • Josef Smolen
    • 2
  • Walter Knapp
    • 1
  1. 1.Institute of ImmunologyUniversity of ViennaViennaAustria
  2. 2.Department of Medicine and Ludwig Boltzmann Institute for RheumatologyKH LainzViennaAustria

Personalised recommendations