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Inflammation

, Volume 10, Issue 1, pp 59–66 | Cite as

Levels of messenger ribonucleic acids for plasma proteins in rat liver during acute experimental inflammation

  • Gerhard Schreiber
  • Angela R. Aldred
  • Tim Thomas
  • Helen E. Birch
  • Phillip W. Dickson
  • Tu Guo-Fen
  • Peter C. Heinrich
  • Wolfgang Northemann
  • Geoffrey J. Howlett
  • Felice A. De Jong
  • Alana Mitchell
Original Articles

Abstract

The levels of mRNA for plasma proteins and for metallothionein in rat liver during the acute-phase response were studied by hybridization to specific cDNA probes. The mRNA forα 2 -macroglobulin, theβ-chain of fibrinogen, α1,-acid glycoprotein (so-called acute-phase reactants) reached a maximum level between 18 and 36 h after inducing an acute inflammation. The level of mRNA for metallothionein-I peaked earlier, after 12 h. The mRNA for transferrin showed a delayed increase with a broad maximum for its relative level after 36–60 h. The mRNA levels for albumin and α2u-globulin (so-called negative acute-phase reactants) decreased, reaching a minimum of 25 % of the normal level after 36 h (albumin) and after 72 h (α2u-globulin). The ratios of the rates of incorporation of leucine into the proteins over the levels of their mRNA in liver changed only a little, indicating that the rates of synthesis of plasma proteins in the liver are regulated at the mRNA level during the acute-phase response to inflammation.

Keywords

mRNA Level Leucine Fibrinogen Plasma Protein Transferrin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Corporation 1986

Authors and Affiliations

  • Gerhard Schreiber
    • 1
  • Angela R. Aldred
    • 1
  • Tim Thomas
    • 1
  • Helen E. Birch
    • 1
  • Phillip W. Dickson
    • 1
  • Tu Guo-Fen
    • 1
  • Peter C. Heinrich
    • 2
  • Wolfgang Northemann
    • 2
  • Geoffrey J. Howlett
    • 1
  • Felice A. De Jong
    • 1
  • Alana Mitchell
    • 1
  1. 1.The Russell Grimwade School of BiochemistryUniversity of MelbourneParkvilleAustralia
  2. 2.Biochemisches InstitutUniversität Freiburg, i.Br.FreiburgFederal Republic of Germany

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