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Human polymorphonuclear leukocytes release leukotriene B4 during phagocytosis ofStaphylococcus aureus

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We studied release of leukotriene B4 (LTB4) by human polymorphonuclear leukocytes (PMNs) during phagocytosis of staphylococci in the presence or absence of arachidonic acid. The 12×107 PMNs incubated with 3×109 opsonizedS. aureus and 50μM arachidonic acid released 1.45±0.42 nmol LTB4. No LTB4 was detected after stimulation of PMNs withS. aureus or arachidonic acid by themselves. However, by increasing the concentration of arachidonic acid to 200 or 400μM, 1.22±0.45 and 1.98±0.49 nmol LTB4, respectively, was released by PMNs. The effect of different bacteria-PMN ratios on LTB4 production was also studied. LTB4 varied from 0.3 to 2.0 nmol when bacteria/PMN ratios increased from 5 to 50 (respectively) in the presence of 50 μM arachidonic acid. Thus, phagocytizing PMNs produce LTB4 in the presence of arachidonic acid, and its production is dependent on the number of bacteria phagocytized.

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Henricks, P.A.J., Van Der Tol, M.E., Engels, F. et al. Human polymorphonuclear leukocytes release leukotriene B4 during phagocytosis ofStaphylococcus aureus . Inflammation 10, 37–47 (1986). https://doi.org/10.1007/BF00916039

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  • Public Health
  • Internal Medicine
  • Arachidonic Acid
  • Polymorphonuclear Leukocyte
  • LTB4