Abstract
Rheumatoid synovial fluids generated significantly greater amounts of superoxide, lysosomal enzymes, and superoxide dismutase from neutrophils into extracellular fluid than osteoarthritic synovial fluids. Rheumatoid factors isolated from serum suppressed superoxide-generating activity of performed immune complexes, but did not suppress that of intermediate-sized immune complexes isolated from RA serum. Synovial fluid neutrophils has a greater capacity to generate superoxide and lower intracellular superoxide dismutase activity, compared with peripheral neutrophils of the corresponding patients. These results suggest that neutrophil superoxide release may be modulated, both by rheumatoid factor and by intracellular and extracellular superoxide dismutase.
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Tenner, A. J., N. R. Cooper. 1982, Stimulation of human polymorphonuclear leukocytes oxidative response by the subunit of the first component of complements.J. Immunol. 128:2547–2552.
Simchowitz, L., J. P. Atkinson, andI. Spilberg. 1980, Stimulus-specific dactivation of chemotactic factor-induced cyclic AMP response and superoxide generation by human neutrophils.J. Clin. Invest. 66:736–747.
Godlstein, I. M., D. Roos, H. B. Kaplan, andG. Weissman. 1975. Complement and immunoglobulins stimulate superoxide production by human leukocytes independently of phagocytosis.J. Clin. Invest. 56:1155–1163.
Weiss, S. J., andP. A. Ward. 1982. Immune complex induced generation of oxygen metabolites by human neutrophils.J. Immunol. 129:309–313.
McCord, J. M. 1974. Free radicals and inflammation: Protection of synovial fluid by superoxide dismutase.Science 185:529–531.
Greewald, R. A., andW. W. Moy. 1979. Inhibition of collagen gelation by action of the superoxide radical.Arthritis Rheum. 22:251–259.
Bates, E. J., D. A. Lowther, andC. J. Handley. 1984. Oxygen free-radicals mediate an inhibition of proteoglycan synthesis in cultured articular cartilage.Ann. Rheum. Dis. 43:462–469.
Sacks, T., C. F. Moldow, P. R. Craddock, T. K. Bowers, H. S. Jacob. 1978. Oxygen radicals mediate endothelial cell damage by complement-stimulated granulocytes. An in vitro model of immune vascular damage.J. Clin. Invest. 61:1161–1167.
Harlan, J. M., P. D. Killen, L. A. Harker, G. Striker. 1981. Neutrophil-mediate endothelial injury in vitro. Mechanisms of cell detachment.J. Clin. Invest. 68:1394–1402.
Simon, R. H., C. H. Scoggin, D. Patterson. 1981. Hydrogen peroxide causes the fatal injury to human fibroblasts exposed to oxygen radicals.J. Biol. Chem. 256:7181–7186.
Shingu, M., K. Yoshioka, M. Nobunaga, andK. Yoshida. 1985. Human vascular smooth muscle cells lack catalase activity and are susceptible to hydrogen peroxide.Inflammation 9:309–320.
Shingu, M., andM. Nobunaga. 1984. Chemotactic activity generated in human serum from the fifth component of complement by hydrogen peroxide.Am. J. Pathol. 117:35–40.
Petrone, W. F., D. R. English, K. Wong, andJ. M. McCord 1980. Free radicals and inflammation: Superoxide-dependent activation of a neutrophil chemotactic factor in plasma.Proc. Natl. Acad. Sci. U.S.A.77:1159–1163.
Perez, H. D., B. B. Weksler, andI. M. Goldstein. 1980. Generation of a chemotactic lipid from arachidonic acid by superoxide-generating system.Inflammation 4:313–327.
Weiss, S. J., andS. Regiani. 1984. Neutrophils degrade subendothelial matrices in the presence of alpha-1-proteinase inhibitor. Cooperative use of lysosomal proteinases and oxygen metabolites.J. Clin. Invest. 73:1297–1303.
McCormick, J. R., M. M. Harkin, K. J. Johnson, andP. A. Ward. 1981. Suppression by superoxide dismutase of immune complex-induced pulmonary alveolitis and dermal inflammation.Am. J. Pathol. 102:55–61.
Ward, P. A., G. O. Till, R. Kunkel, andG. Beauchamp. 1983. Evidence for role of hydroxyl radicals in complement and neutrophil-dependent tissue injury.J. Clin. Invest. 72:789–801.
Ropes, M. W., G. A. Bennett, S. Cobb, R. Jacox, andR. A. Jessar. 1958. 1958 revision of diagnostic criteria for rheumatoid arthritis.Bull. Rheum. Dis. 9:175–176.
Boyum, A. 1968. Isolation for mononuclear cells and granulocytes from human blood. Isolation of mononuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1g.Scand. J. Lab. Invest. 21(Suppl. 97):77–89.
Kent, J. F., andE. H. Fife. 1963. Precise standardization of reagents for complement fixation.Am. J. Top. Med. 12:105–116.
Platts-Mills, T. A. E., andK. Ishizaka. 1974. Activation of the alternative pathway of human complement by rabbit cells.J. Immunol. 115:348–358.
Pope, R. M., S. J. McDuffy. 1974. IgG rheumatoid factor relationship to seropositive rheumatoid arthritis and absence in seronegative disorders.Arthritis Rheum. 22:988–998.
Hay, F. C., L. J. Nineham, andI. M. Roitt. 1976. Routine assay forthe detection of immune complexes of known immunoglobulin class using solid phase C1q.Clin. Exp. Immunol. 24:396–400.
Woolen, J. W., R. Heyworth, andP. G. Walker. 1961. Studies on glucosaminidase. 3 testicularn-acetyl-β-galactosaminidase.Biochem. J. 78:111–116.
McCord, J. M., andI. Fridovich. 1969. Superoxide dismutase. An enzyme function for erythrocuprein (hemocuprein).J. Biol. Chem. 244:6049–6055.
Van Gelder, B. F., andE. C. Slater. 1962. The extinction coefficient of cytochromec.Biochem. Biophys. Acta.58:593–595.
Nardella, F. A., D. C. Teller, andM. Mannik. 1981. Studies on the antigenic determinants in the self-association of IgG rheumatoid factor.J. Exp. Med. 154:112–125.
Lowry, O. H., N. J. Rosebrough, A. L. Farr, andR. J. Randall. 1951. Protein measurement with the Polin phenol reagent.J. Biol. Chem. 193:265–275.
Todoroki, T., M. Shingu, N. Nobunaga. 1986. Superoxide generation by synovial fluid neutrophils enhanced by immune complexes and suppressed by rheumatoid factor in synovial fluid.Rheumatology Int. 6:133–137.
Hay, F. C., L. J. Nineham, R. Permal, andI. M. Roitt. 1979. Intraarticular and circulating immune complexes and antiglobulins (IgG and IgM) in rheumatoid arthritis: Correlation with clinical feature,Ann. Rheum. Dis. 38:1–7.
Jalla, J. T., J. E. Volanakis, andR. E. Schrohenloher. 1979. Immune complexes in rheumatoid arthritis sera and synovial fluids.Arthritis Rheum. 22:440–448.
Ruddy, S., K. F. Austen. 1970. The complement system in rheumatoid synovitis. 1. An analysis of complement component activities in rheumatoid synovial fluids.Arthritis Rheum. 13:713–723.
Hunder, G. G., F. C. McDuffie, andB. J. Mullen. 1977. Activation of complement components C3 and factor B in synovial fluids.J. Lab. Clin. Med. 89:160–171.
Gale, R., J. V. Bertouch, T. P. Gordon, J. Bradley, andP. J. Roberts-Thompson. 1984. Neutrophil activation by immune complexes and the role of rheumatoid factor.Ann. Rheum. Dis. 43:34–39.
Gale, R. J., A. Nikoloutsopolos, J. Bradley, andJ. Roberts-Thompson. 1985. Immune complex activation of neutrophils and enhancement of the activation by rheumatoid factor and complement.J. Rheumatol. 12:21–26.
Morrison, A. D., W. Pruzanski, andN. S. Ranadive. 1978. Release of lysosomal enzymes from human polymorphonuclear leukocytes by soluble intermediate immune complexes.Scand. J. Rheumatol. 7:241–246.
Ward, P. A., andN. J. Zvaifler. 1973. Quantitative phagocytosis by neutrophils. 11. Release of the C5-cleaving enzyme and inhibition of phagocytosis by rheumatoid factor.J. 1mmunol. 111:1777–1782.
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Shingu, M., Todoroki, T. & Nobunaga, M. Generation of superoxide by immunologically stimulated normal human neutrophils and possible modulation by intracellular and extracellular sod and rheumatoid factors. Inflammation 11, 143–151 (1987). https://doi.org/10.1007/BF00916016
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DOI: https://doi.org/10.1007/BF00916016