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Influence of neutrophil cationic proteins on generation of superoxide by human polymorphonuclear cells during phagocytosis

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The cationic proteins from neutrophyl lysosomes have been shown to modulate phagocytic activity of granulocytes. The present study reports the effects of the cationic protein fractions on the generation of O 2 by human PMNs during phagocytosis. Human PMNs were reacted win different phagocytic stimuli in the presence and absence of lysosomal cationic proteins and the amount of O 2 generated was determined by superoxide dismutase inhibitable reduction of cytochromec. Total cationic protein extract from neutrophil lysosomes enhanced O 2 generated by PMNs during the phagocytosis of IgG-coated latex beads and opsonized zymosan particles. The analysis of the fractions of cationic proteins obtained from a Sephadex G-75 column showed that the O 2 generation-enhancing activity was associated with the proteins eluted in fractions III and IV. A protein fraction mainly eluted in void volume inhibited the cytochromec reduction by O 2 formed during phagocytosis. This was due to the presence of superoxide dismutase-like activity since O 2 generated by the xanthine-xanthine oxidase system was also inhibited by this fraction. The cationic protein fractions III and IV from the Sephadex G-75 column were further subfractionated. Although the O 2 -enhancing activity was eluted in the same fractions as chymotrypsin activity, there was no quantitative correlation between the amount of O 2 generation and chymotrypsin activity. Moreover, commercial chymotrypsin did not enhance O 2 generation. Electrophoretic analysis of the isolated protein fractions suggests that O 2 generation enhancing protein (SGEP) is different from lysozyme or chymotrypsin and probably represents previously undescribed protein.

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Alam, M., Ranadive, N.S. & Pruzanski, W. Influence of neutrophil cationic proteins on generation of superoxide by human polymorphonuclear cells during phagocytosis. Inflammation 11, 131–142 (1987).

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  • Chymotrypsin
  • Cationic Protein
  • Latex Bead
  • Chymotrypsin Activity
  • Opsonized Zymosan