Inflammation

, Volume 14, Issue 3, pp 337–353 | Cite as

Effects of chemotactic peptide f-met-leu-phe (FMLP) on C3b receptor (CR1) expression and phagocytosis of microspheres by human neutrophils

  • James D. Ogle
  • J. Greg Noel
  • R. Michael Sramkoski
  • Cora K. Ogle
  • J. Wesley Alexander
Original Articles

Abstract

FMLP caused maximal upregulation of CR1 on neutrophils at a concentration of 10−8 M but caused maximal enhancement of CR1-dependent phagocytosis of C3b · IgG-coated microspheres only at a concentration of 10−6 M. There were positive correlations between FMLP-mediated upregulation of CR1 and FMLP-mediated enhancement of phagocytosis (correlation coefficient=0.73, slope=2.2) and between FMLP-mediated upregulation of CR1 and FMLP-mediated increase in total cell-associated microspheres (correlation coefficient=0.88, slope=1.3). The phagocytic capacity of both untreated and 10−6 M FMLP-treated neutrophils was completely inhibited by fluid phase C3b and partially inhibited by aggregated IgG. The data suggest that CR1 upregulation is required but is not sufficient for maximal phagocytosis by the leukocytes. The data also suggest that FMLP at the higher concentrations may impart a phagocytic function to CR1, activate other phagocytic receptors, elicit phagocytosis—inducing mediators or may elicit a separate mechanism of phagocytosis. During the study, it was observed that there was considerable individual variation among different neutrophil preparations with respect to CR1 expression and binding and phagocytic capacity.

Keywords

Public Health Peptide Internal Medicine Individual Variation Fluid Phase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Plenum Publishing Corporation 1990

Authors and Affiliations

  • James D. Ogle
    • 1
    • 3
  • J. Greg Noel
    • 3
  • R. Michael Sramkoski
    • 3
  • Cora K. Ogle
    • 2
    • 3
  • J. Wesley Alexander
    • 2
    • 3
  1. 1.Departments of Molecular Genetics, Biochemistry and MicrobiologyUniversity of Cincinnati College of MedicineUSA
  2. 2.Department of SurgeryUniversity of Cincinnati College of MedicineUSA
  3. 3.Cincinnati UnitShriners Burns InstituteCincinnati

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